ID A0A0E9N2K1_9BACT Unreviewed; 497 AA.
AC A0A0E9N2K1;
DT 24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT 24-JUN-2015, sequence version 1.
DT 27-MAR-2024, entry version 20.
DE SubName: Full=Putative glycosidase {ECO:0000313|EMBL:GAO43866.1};
GN ORFNames=FPE01S_02_09720 {ECO:0000313|EMBL:GAO43866.1};
OS Flavihumibacter petaseus NBRC 106054.
OC Bacteria; Bacteroidota; Chitinophagia; Chitinophagales; Chitinophagaceae;
OC Flavihumibacter.
OX NCBI_TaxID=1220578 {ECO:0000313|EMBL:GAO43866.1, ECO:0000313|Proteomes:UP000033121};
RN [1] {ECO:0000313|EMBL:GAO43866.1, ECO:0000313|Proteomes:UP000033121}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 106054 {ECO:0000313|EMBL:GAO43866.1,
RC ECO:0000313|Proteomes:UP000033121};
RA Miyazawa S., Hosoyama A., Hashimoto M., Noguchi M., Tsuchikane K., Ohji S.,
RA Yamazoe A., Ichikawa N., Kimura A., Fujita N.;
RT "Whole genome shotgun sequence of Flavihumibacter petaseus NBRC 106054.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Glycan metabolism; L-arabinan degradation.
CC {ECO:0000256|ARBA:ARBA00004834}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865, ECO:0000256|RuleBase:RU361187}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAO43866.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BBWV01000002; GAO43866.1; -; Genomic_DNA.
DR RefSeq; WP_052955816.1; NZ_BBWV01000002.1.
DR AlphaFoldDB; A0A0E9N2K1; -.
DR STRING; 1220578.FPE01S_02_09720; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000033121; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd08998; GH43_Arb43a-like; 1.
DR Gene3D; 2.40.128.10; -; 1.
DR InterPro; IPR032291; Abn2_C.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR43301; ARABINAN ENDO-1,5-ALPHA-L-ARABINOSIDASE; 1.
DR PANTHER; PTHR43301:SF3; ARABINOSIDASE-RELATED; 1.
DR Pfam; PF16369; GH43_C; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|RuleBase:RU361187, ECO:0000313|EMBL:GAO43866.1};
KW Hydrolase {ECO:0000256|RuleBase:RU361187};
KW Reference proteome {ECO:0000313|Proteomes:UP000033121};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..497
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5002430154"
FT DOMAIN 381..494
FT /note="Extracellular endo-alpha-(1->5)-L-arabinanase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF16369"
FT ACT_SITE 74
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 261
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 202
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 497 AA; 55222 MW; 85B337921FEE1C6A CRC64;
MKTIGKKLVA AGVLFLLLQG NTSCSKHGDE PVPVDTTIDP PVQPVFDINS ISDTYESLAP
FANYLHWGSY NVHDPSVIKV GEYYYCYSTD AGFGISVRSG IQIRRSKDLV VWEYMGWVFN
ALPAQGAAFI QSKGGTAFDA LWAPYVLKSG NEYRLYYSLS SAKPRLSVIG MATASSPMGP
WTEKGLVVTS LDDASIQTNA IDPSVITLAN GNQYLYYGSA WDGIYVLQLN AATGLALKNA
DKGKRIANRG FTDGRYNGNI EGAEIIYNAA QQQYYLFIAY DWLQTKYNVR VCRSANPEGP
FYDFNGKDAN SNEDHGPMIL APYQFEGHAG WQGVAHCSVF QDGNGQYFIA HQGRPVVNSY
YMDLHVRKIF WTPDGWPVVS PERFAWEDNA VVDQSMLPGS WEKIELMYSV VPGYADEQKL
PDLQVAKKIS IDVGGTLNGE AGSSWTYQAP WLEMKWKNGG NEKVLVQKGR DWENRIQNSL
IFTGLNNEGT AVWGKKH
//
