ID   A0A0F5MNK1_9RICK        Unreviewed;       445 AA.
AC   A0A0F5MNK1;
DT   24-JUN-2015, integrated into UniProtKB/TrEMBL.
DT   24-JUN-2015, sequence version 1.
DT   27-MAR-2024, entry version 31.
DE   RecName: Full=RNA polymerase sigma-54 factor {ECO:0000256|PIRNR:PIRNR000774};
GN   Name=rpoN1 {ECO:0000313|EMBL:KKB96149.1};
GN   ORFNames=SZ25_00774 {ECO:0000313|EMBL:KKB96149.1};
OS   Candidatus Arcanobacter lacustris.
OC   Bacteria; Pseudomonadota; Alphaproteobacteria; Rickettsiales; Arcanobacter.
OX   NCBI_TaxID=1607817 {ECO:0000313|EMBL:KKB96149.1, ECO:0000313|Proteomes:UP000033358};
RN   [1] {ECO:0000313|EMBL:KKB96149.1, ECO:0000313|Proteomes:UP000033358}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=SCGC AAA041-L04 {ECO:0000313|EMBL:KKB96149.1,
RC   ECO:0000313|Proteomes:UP000033358};
RA   Martijn J., Schulz F., Zaremba-Niedzwiedzka K., Viklund J.,
RA   Stepanauskas R., Andersson S.G.E., Horn M., Guy L., Ettema T.J.G.;
RT   "Single cell genomics of a rare environmental alphaproteobacterium provides
RT   unique insights into Rickettsiaceae evolution.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Sigma factors are initiation factors that promote the
CC       attachment of RNA polymerase to specific initiation sites and are then
CC       released. {ECO:0000256|PIRNR:PIRNR000774}.
CC   -!- SIMILARITY: Belongs to the sigma-54 factor family.
CC       {ECO:0000256|ARBA:ARBA00008798, ECO:0000256|PIRNR:PIRNR000774}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KKB96149.1}.
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DR   EMBL; JYHA01000127; KKB96149.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0F5MNK1; -.
DR   PATRIC; fig|1607817.3.peg.774; -.
DR   Proteomes; UP000033358; Unassembled WGS sequence.
DR   GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0001216; F:DNA-binding transcription activator activity; IEA:InterPro.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-KW.
DR   GO; GO:0006352; P:DNA-templated transcription initiation; IEA:InterPro.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.10.1330; RNA polymerase sigma-54 factor, core-binding domain; 1.
DR   InterPro; IPR000394; RNA_pol_sigma_54.
DR   InterPro; IPR007046; RNA_pol_sigma_54_core-bd.
DR   InterPro; IPR007634; RNA_pol_sigma_54_DNA-bd.
DR   InterPro; IPR038709; RpoN_core-bd_sf.
DR   NCBIfam; TIGR02395; rpoN_sigma; 1.
DR   PANTHER; PTHR32248; RNA POLYMERASE SIGMA-54 FACTOR; 1.
DR   PANTHER; PTHR32248:SF4; RNA POLYMERASE SIGMA-54 FACTOR; 1.
DR   Pfam; PF00309; Sigma54_AID; 1.
DR   Pfam; PF04963; Sigma54_CBD; 1.
DR   Pfam; PF04552; Sigma54_DBD; 1.
DR   PIRSF; PIRSF000774; RpoN; 1.
DR   PRINTS; PR00045; SIGMA54FCT.
DR   PROSITE; PS00718; SIGMA54_2; 1.
DR   PROSITE; PS50044; SIGMA54_3; 1.
PE   3: Inferred from homology;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR000774};
KW   DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW   ECO:0000256|PIRNR:PIRNR000774};
KW   Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW   ECO:0000256|PIRNR:PIRNR000774};
KW   Reference proteome {ECO:0000313|Proteomes:UP000033358};
KW   Sigma factor {ECO:0000256|ARBA:ARBA00023082,
KW   ECO:0000256|PIRNR:PIRNR000774};
KW   Transcription {ECO:0000256|ARBA:ARBA00023163,
KW   ECO:0000256|PIRNR:PIRNR000774};
KW   Transcription regulation {ECO:0000256|ARBA:ARBA00023015,
KW   ECO:0000256|PIRNR:PIRNR000774};
KW   Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PIRNR:PIRNR000774}.
FT   DOMAIN          89..269
FT                   /note="RNA polymerase sigma factor 54 core-binding"
FT                   /evidence="ECO:0000259|Pfam:PF04963"
FT   DOMAIN          284..440
FT                   /note="RNA polymerase sigma factor 54 DNA-binding"
FT                   /evidence="ECO:0000259|Pfam:PF04552"
SQ   SEQUENCE   445 AA;  51405 MW;  294694E5C0BB85B4 CRC64;
     MSNKPSLSIK QGQQLQISNS LQQAIKLLQL PTIELVEFLA LEIEKNPLLS QQDEDNEQEE
     LPVETSELRY QKVHKYNYGD DYNIEDNESG KTLREHVLEQ IYLDIHDKHK RMIALNLADL
     LTEAGYIEED LTLIATKLGV EFKELEEVLY LMQKFDPPGI FARNLAECLR LQLQDLGKLD
     ESYELLLENL QLISSGGAAV FKKTHHIADE DFALMIKNLK SLNPKPGSKF IPQITNYMEP
     EVFVRREKNR LIVELNNNIL PKILVNRRYY AEIRKKTVNQ QEKNYLSEQL STANWLIKAL
     DQRANTLLRV ATEIISRQHE FFDRGINYLR PMTIKDISSA IDLHESSISR ITSNKFMSTP
     SGLFEMKYFF SSGVGGENTE DDYSSTTIKH LIKKMIDEEE KPLSDNQIAK ALKEQNINIA
     RRTVTKYRES LNIPTSALRK IAHYV
//