ID A0A0F7HCA1_SERFO Unreviewed; 200 AA.
AC A0A0F7HCA1;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=3-isopropylmalate dehydratase small subunit {ECO:0000256|HAMAP-Rule:MF_01031};
DE EC=4.2.1.33 {ECO:0000256|HAMAP-Rule:MF_01031};
DE AltName: Full=Alpha-IPM isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
DE Short=IPMI {ECO:0000256|HAMAP-Rule:MF_01031};
DE AltName: Full=Isopropylmalate isomerase {ECO:0000256|HAMAP-Rule:MF_01031};
GN Name=leuD {ECO:0000256|HAMAP-Rule:MF_01031,
GN ECO:0000313|EMBL:AKG69769.1};
GN Synonyms=leuD1 {ECO:0000313|EMBL:VTR26333.1};
GN ORFNames=BSQ40_05050 {ECO:0000313|EMBL:OKP30571.1}, H8I91_07145
GN {ECO:0000313|EMBL:MBC3250031.1}, NCTC12965_02343
GN {ECO:0000313|EMBL:VTR26333.1}, WN53_11965
GN {ECO:0000313|EMBL:AKG69769.1};
OS Serratia fonticola.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=47917 {ECO:0000313|EMBL:AKG69769.1, ECO:0000313|Proteomes:UP000034699};
RN [1] {ECO:0000313|EMBL:AKG69769.1, ECO:0000313|Proteomes:UP000034699}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 4576 {ECO:0000313|EMBL:AKG69769.1,
RC ECO:0000313|Proteomes:UP000034699};
RA Chan K.-G., Ee R.;
RT "Complete Genome Sequencing of Serratia Fonticola DSM-4576.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OKP30571.1, ECO:0000313|Proteomes:UP000186436}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=5l {ECO:0000313|EMBL:OKP30571.1,
RC ECO:0000313|Proteomes:UP000186436};
RA Goncharov A., Grigoriev S., Azarov D., Kolodzhieva V., Tikhonov A.,
RA Akhremenko Y., Tarasova L., Zueva L., Suvorov A.;
RT "Draft genome sequence of Serratia fonticola 5l isolated from intestinal
RT tract content of a 9000-year-old elk (Alces alces) from permafrost on the
RT Omoloy river.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:VTR26333.1, ECO:0000313|Proteomes:UP000358076}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC12965 {ECO:0000313|EMBL:VTR26333.1,
RC ECO:0000313|Proteomes:UP000358076};
RG Pathogen Informatics;
RL Submitted (MAY-2019) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:MBC3250031.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UPMP2131 {ECO:0000313|EMBL:MBC3250031.1};
RA Richter L., Du Plessis E.M., Duvenage S., Allam M., Korsten L.;
RT "Food and environmental bacterial isolates.";
RL Submitted (AUG-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the isomerization between 2-isopropylmalate and 3-
CC isopropylmalate, via the formation of 2-isopropylmaleate.
CC {ECO:0000256|ARBA:ARBA00002695, ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate;
CC Xref=Rhea:RHEA:32287, ChEBI:CHEBI:1178, ChEBI:CHEBI:35121;
CC EC=4.2.1.33; Evidence={ECO:0000256|ARBA:ARBA00000491,
CC ECO:0000256|HAMAP-Rule:MF_01031};
CC -!- PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine
CC from 3-methyl-2-oxobutanoate: step 2/4. {ECO:0000256|ARBA:ARBA00004729,
CC ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- SUBUNIT: Heterodimer of LeuC and LeuD. {ECO:0000256|ARBA:ARBA00011271,
CC ECO:0000256|HAMAP-Rule:MF_01031}.
CC -!- SIMILARITY: Belongs to the LeuD family. LeuD type 1 subfamily.
CC {ECO:0000256|ARBA:ARBA00009845, ECO:0000256|HAMAP-Rule:MF_01031}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP011254; AKG69769.1; -; Genomic_DNA.
DR EMBL; JACNYM010000005; MBC3250031.1; -; Genomic_DNA.
DR EMBL; MQRH01000003; OKP30571.1; -; Genomic_DNA.
DR EMBL; CABEEZ010000044; VTR26333.1; -; Genomic_DNA.
DR RefSeq; WP_024483541.1; NZ_QRAU01000005.1.
DR STRING; 47917.AV650_07525; -.
DR GeneID; 30320886; -.
DR KEGG; sfw:WN53_11965; -.
DR PATRIC; fig|47917.8.peg.2478; -.
DR OrthoDB; 9777465at2; -.
DR UniPathway; UPA00048; UER00071.
DR Proteomes; UP000034699; Chromosome.
DR Proteomes; UP000186436; Unassembled WGS sequence.
DR Proteomes; UP000358076; Unassembled WGS sequence.
DR Proteomes; UP000596701; Unassembled WGS sequence.
DR GO; GO:0009316; C:3-isopropylmalate dehydratase complex; IEA:InterPro.
DR GO; GO:0003861; F:3-isopropylmalate dehydratase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR GO; GO:0009098; P:leucine biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd01577; IPMI_Swivel; 1.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR HAMAP; MF_01031; LeuD_type1; 1.
DR InterPro; IPR004431; 3-IsopropMal_deHydase_ssu.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR InterPro; IPR033940; IPMI_Swivel.
DR NCBIfam; TIGR00171; leuD; 1.
DR PANTHER; PTHR43345:SF5; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT; 1.
DR PANTHER; PTHR43345; 3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
PE 3: Inferred from homology;
KW Amino-acid biosynthesis {ECO:0000256|ARBA:ARBA00022605, ECO:0000256|HAMAP-
KW Rule:MF_01031};
KW Branched-chain amino acid biosynthesis {ECO:0000256|ARBA:ARBA00023304,
KW ECO:0000256|HAMAP-Rule:MF_01031}; Isomerase {ECO:0000313|EMBL:AKG69769.1};
KW Leucine biosynthesis {ECO:0000256|ARBA:ARBA00022430, ECO:0000256|HAMAP-
KW Rule:MF_01031};
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01031};
KW Reference proteome {ECO:0000313|Proteomes:UP000034699}.
FT DOMAIN 1..125
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
SQ SEQUENCE 200 AA; 22412 MW; 2211B9C136201327 CRC64;
MAKFTQHTGL VVPLDAANVD TDAIIPKQFL QKVTRTGFGQ HLFNDWRFLD DAGQQPNPEF
VLNLPRYKGA SILLARENFG CGSSREHAPW ALTDYGFKVV IAPSFADIFY GNSFNNQLLP
VTLSEQDVDT LFKLVAEHEG TEFVVDLENQ TVNAGGKSYP FEIDSFRRHC MINGLDSIGL
TLQHEAEISR YEAQQPAFLN
//
