ID A0A0G0MSC5_9BACT Unreviewed; 287 AA.
AC A0A0G0MSC5;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=Nitrogen fixation protein NifU {ECO:0000256|ARBA:ARBA00015278, ECO:0000256|PIRNR:PIRNR000375};
GN ORFNames=UT30_C0032G0005 {ECO:0000313|EMBL:KKR03326.1};
OS Candidatus Uhrbacteria bacterium GW2011_GWF2_39_13.
OC Bacteria; Candidatus Uhrbacteria.
OX NCBI_TaxID=1618995 {ECO:0000313|EMBL:KKR03326.1, ECO:0000313|Proteomes:UP000033935};
RN [1] {ECO:0000313|EMBL:KKR03326.1, ECO:0000313|Proteomes:UP000033935}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- FUNCTION: May be involved in the formation or repair of [Fe-S] clusters
CC present in iron-sulfur proteins. {ECO:0000256|PIRNR:PIRNR000375}.
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 Fe cation per subunit. {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000256|PIRSR:PIRSR000375-1};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000256|PIRSR:PIRSR000375-1};
CC -!- SIMILARITY: Belongs to the NifU family. {ECO:0000256|ARBA:ARBA00006420,
CC ECO:0000256|PIRNR:PIRNR000375}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKR03326.1}.
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DR EMBL; LBWG01000032; KKR03326.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G0MSC5; -.
DR PATRIC; fig|1618995.3.peg.1000; -.
DR Proteomes; UP000033935; Unassembled WGS sequence.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR GO; GO:0009399; P:nitrogen fixation; IEA:UniProtKB-KW.
DR CDD; cd06664; IscU_like; 1.
DR CDD; cd19947; NifU_Fer2_BFD-like; 1.
DR Gene3D; 3.90.1010.10; -; 1.
DR Gene3D; 1.10.10.1100; BFD-like [2Fe-2S]-binding domain; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR InterPro; IPR007419; BFD-like_2Fe2S-bd_dom.
DR InterPro; IPR041854; BFD-like_2Fe2S-bd_dom_sf.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR016217; N_fixation_NifU.
DR InterPro; IPR010238; NIF_FeS_clus_asmbl_NifU.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR InterPro; IPR002871; NIF_FeS_clus_asmbl_NifU_N.
DR NCBIfam; TIGR02000; NifU_proper; 1.
DR PANTHER; PTHR10093:SF8; IRON-SULFUR CLUSTER ASSEMBLY ENZYME ISCU, MITOCHONDRIAL; 1.
DR PANTHER; PTHR10093; IRON-SULFUR CLUSTER ASSEMBLY ENZYME NIFU HOMOLOG; 1.
DR Pfam; PF04324; Fer2_BFD; 1.
DR Pfam; PF01106; NifU; 1.
DR Pfam; PF01592; NifU_N; 1.
DR PIRSF; PIRSF000375; NifU; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF82649; SufE/NifU; 1.
PE 3: Inferred from homology;
KW 2Fe-2S {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron {ECO:0000256|PIRSR:PIRSR000375-1};
KW Iron-sulfur {ECO:0000256|PIRSR:PIRSR000375-1};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR000375-1};
KW Nitrogen fixation {ECO:0000256|ARBA:ARBA00023231,
KW ECO:0000256|PIRNR:PIRNR000375}.
FT DOMAIN 4..122
FT /note="NIF system FeS cluster assembly NifU N-terminal"
FT /evidence="ECO:0000259|Pfam:PF01592"
FT DOMAIN 137..186
FT /note="BFD-like [2Fe-2S]-binding"
FT /evidence="ECO:0000259|Pfam:PF04324"
FT DOMAIN 218..284
FT /note="NIF system FeS cluster assembly NifU C-terminal"
FT /evidence="ECO:0000259|Pfam:PF01106"
FT BINDING 35
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 61
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 105
FT /ligand="Fe cation"
FT /ligand_id="ChEBI:CHEBI:24875"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 139
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 141
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 174
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
FT BINDING 177
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000256|PIRSR:PIRSR000375-1"
SQ SEQUENCE 287 AA; 31510 MW; DD3810FD9F30A1F2 CRC64;
MWDYSKKVMD HFLNPRNVGE IKDADAIAEV GNITCGDSLK LFLKLDENGR IAEAKFKTFG
CASAIASSSA LTELVKGMTL DEAAEVTNKD IVALLGELPE EKMHCSVMGM EALQLAIANY
RGVTLDGHGN DEHEGRIVCR CFNVTDTKIR KLAKENNLHT VEEITNYSKA GGACGTCLDE
IQLILDGIWK ESKSAEDVGG EEKPDFKSMP IVKKVLRVQE VIDKEIRPLL EKDGGSIELV
DIVGNKAIVR LKGRCAVCQT SQVTLKKTVE QKLREFVSPE IEVEGAK
//
