ID A0A0G1LKL0_9BACT Unreviewed; 285 AA.
AC A0A0G1LKL0;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 24-JAN-2024, entry version 20.
DE RecName: Full=Type-2 restriction enzyme {ECO:0000256|PIRNR:PIRNR016080};
DE EC=3.1.21.4 {ECO:0000256|PIRNR:PIRNR016080};
GN ORFNames=UW97_C0011G0005 {ECO:0000313|EMBL:KKT96451.1};
OS Parcubacteria group bacterium GW2011_GWA2_45_15.
OC Bacteria.
OX NCBI_TaxID=1618833 {ECO:0000313|EMBL:KKT96451.1, ECO:0000313|Proteomes:UP000034700};
RN [1] {ECO:0000313|EMBL:KKT96451.1, ECO:0000313|Proteomes:UP000034700}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown C.T., Hug L.A., Thomas B.C., Sharon I., Castelle C.J., Singh A.,
RA Wilkins M.J., Williams K.H., Banfield J.F.;
RT "rRNA introns, odd ribosomes, and small enigmatic genomes across a large
RT radiation of phyla.";
RL Nature 0:0-0(2015).
CC -!- FUNCTION: A P subtype restriction enzyme that recognizes the double-
CC stranded unmethylated sequence 5'-GATC-3'.
CC {ECO:0000256|PIRNR:PIRNR016080}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage of DNA to give specific double-
CC stranded fragments with terminal 5'-phosphates.; EC=3.1.21.4;
CC Evidence={ECO:0000256|PIRNR:PIRNR016080};
CC -!- SIMILARITY: Belongs to the DpnII type II restriction endonuclease
CC family. {ECO:0000256|PIRNR:PIRNR016080}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKT96451.1}.
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DR EMBL; LCKK01000011; KKT96451.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G1LKL0; -.
DR PATRIC; fig|1618833.3.peg.222; -.
DR Proteomes; UP000034700; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0009036; F:type II site-specific deoxyribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-UniRule.
DR InterPro; IPR021191; Restrct_endonuc_II_DpnII.
DR InterPro; IPR007637; Restrct_endonuc_II_DpnII-like.
DR Pfam; PF04556; DpnII; 1.
DR PIRSF; PIRSF016080; Restrict_endonuc_II_DpmII; 1.
PE 3: Inferred from homology;
KW Endonuclease {ECO:0000256|PIRNR:PIRNR016080};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR016080};
KW Nuclease {ECO:0000256|PIRNR:PIRNR016080};
KW Restriction system {ECO:0000256|PIRNR:PIRNR016080}.
FT DOMAIN 4..281
FT /note="Restriction endonuclease type II DpnII-like"
FT /evidence="ECO:0000259|Pfam:PF04556"
SQ SEQUENCE 285 AA; 32856 MW; EDD5468CADDFFEE0 CRC64;
MNKDFNNLIT TFKSSIKTWD YFVNWKKVFS NSSDLEISLN KLNYLLGKDN LEDEFRKLYA
SNPDIVKALP VLLAVRENKL EVFDKATKNS EFFDFSGKEQ DPEKYLEFLN KSGLARLFQK
DGVKNLVDYV MGVEVGLDSN GRKNRGGSLM EEIAGAFVSD FCNKNGYEYM AQARATNIKS
KWGVDIKVDK SERSFDFAVY NPKNKKIKLF EANFYNGGGS KLKAVCGEFR SLYDELKAQD
IDFIWITDGL GWHTTKRPLE ETYNHNEYVF NLNMLESGVL NELKW
//
