ID A0A0G2GGI4_9PEZI Unreviewed; 886 AA.
AC A0A0G2GGI4;
DT 22-JUL-2015, integrated into UniProtKB/TrEMBL.
DT 22-JUL-2015, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=dihydroorotase {ECO:0000256|ARBA:ARBA00012860};
DE EC=3.5.2.3 {ECO:0000256|ARBA:ARBA00012860};
GN ORFNames=UCDDS831_g03453 {ECO:0000313|EMBL:KKY22738.1};
OS Diplodia seriata.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetes incertae sedis; Botryosphaeriales; Botryosphaeriaceae;
OC Diplodia.
OX NCBI_TaxID=420778 {ECO:0000313|EMBL:KKY22738.1, ECO:0000313|Proteomes:UP000034182};
RN [1] {ECO:0000313|EMBL:KKY22738.1, ECO:0000313|Proteomes:UP000034182}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DS831 {ECO:0000313|EMBL:KKY22738.1};
RA Morales-Cruz A., Amrine K.C., Cantu D.;
RL Submitted (MAR-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KKY22738.1, ECO:0000313|Proteomes:UP000034182}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DS831 {ECO:0000313|EMBL:KKY22738.1};
RA Lawrence D.P., Travadon R., Rolshausen P.E., Baumgartner K.;
RT "Distinctive expansion of gene families associated with plant cell wall
RT degradation and secondary metabolism in the genomes of grapevine trunk
RT pathogens.";
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC (S)-dihydroorotate from bicarbonate: step 3/3.
CC {ECO:0000256|ARBA:ARBA00004880}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|RuleBase:RU003945}; Multi-
CC pass membrane protein {ECO:0000256|RuleBase:RU003945}.
CC -!- SIMILARITY: Belongs to the OXA1/ALB3/YidC family.
CC {ECO:0000256|RuleBase:RU003945}.
CC -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC DHOase family. Class II DHOase subfamily.
CC {ECO:0000256|ARBA:ARBA00005631}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KKY22738.1}.
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DR EMBL; LAQI01000073; KKY22738.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G2GGI4; -.
DR UniPathway; UPA00070; UER00117.
DR Proteomes; UP000034182; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004151; F:dihydroorotase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019856; P:pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR CDD; cd20069; 5TM_Oxa1-like; 1.
DR Gene3D; 3.20.20.140; Metal-dependent hydrolases; 1.
DR HAMAP; MF_00219; PyrC_classII; 1.
DR InterPro; IPR006680; Amidohydro-rel.
DR InterPro; IPR004721; DHOdimr.
DR InterPro; IPR002195; Dihydroorotase_CS.
DR InterPro; IPR032466; Metal_Hydrolase.
DR InterPro; IPR028055; YidC/Oxa/ALB_C.
DR NCBIfam; TIGR00856; pyrC_dimer; 1.
DR PANTHER; PTHR43137; DIHYDROOROTASE; 1.
DR PANTHER; PTHR43137:SF1; DIHYDROOROTASE; 1.
DR Pfam; PF02096; 60KD_IMP; 1.
DR Pfam; PF01979; Amidohydro_1; 1.
DR SUPFAM; SSF51556; Metallo-dependent hydrolases; 1.
DR PROSITE; PS00482; DIHYDROOROTASE_1; 1.
DR PROSITE; PS00483; DIHYDROOROTASE_2; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Pyrimidine biosynthesis {ECO:0000256|ARBA:ARBA00022975};
KW Transmembrane {ECO:0000256|RuleBase:RU003945, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 315..333
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 159..352
FT /note="Membrane insertase YidC/Oxa/ALB C-terminal"
FT /evidence="ECO:0000259|Pfam:PF02096"
FT DOMAIN 523..785
FT /note="Amidohydrolase-related"
FT /evidence="ECO:0000259|Pfam:PF01979"
FT REGION 396..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 396..410
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 886 AA; 96583 MW; 115335F1521E3528 CRC64;
MMKSRGLRPA NCARLALQNP LRAPRQFSTH ASLRAPVLLR ARNPSLDLRS ALNAPGLRTP
FAVGSAASAI RFASTSPAAA TSSVLENSKA AAADATSSAP VEAAASSVDG VTDEFLANLA
NMPEQIGYLK AVGLDYGWGP TSCIEWLLEH VHIWMGIPWW SSIVVTAIIV RTSLFPLFAR
TSDVTARQQA LKEITDPLNA KMNAARIAGN TDQMMMARTE LMGVYKHAGI NPWKAFYAPV
AQAVTGYCTW KLLRAMAALP VPGLENGGFL WLSDLTVPDP WFILPISFGA LIHVVAKSGG
ETGALKQFTG LQKKFLFYFM PGLAILFTIA QPATVQFSFF AASTLGMLQA KLLRSPTVRD
WLSLAPIVGG PQPNPSNPGV IDVQARVTSP GEFKWEPPTV QSSISKSGPI NKKINKKPVS
KGPFDGLKKS YMETRQELQD FKDGIMKRAG MYEDNTKGRT QSKQFLKRAE EYENRRQAER
DMDFFSIGRA VDFMCIYLYT DDAFVHTADD PTMPLDKFDG LELPAAADMH VHLRDNAMME
TVTPTIRQGG VNTVYVMLTR PLSSLCPGQP NLVPPLTTVD ATLAYRARLQ AVEPNVTFLS
SLYLHPDITP DTIVAAKKAG VTGVKSYPAG VTTNSAAGVV DYAAFYPVFA EMERQDMVLN
LHGELPPSAA SADAEDITVL NAEERFLPTL RELHARFPRL RIILEHCTSK AAIEAVEACG
PTVAATITAH HLYLTVDDVV GNAFHFCKPV AKLPADRLAL LRAAARGGSK FFFGTDSAPH
PITAKSGPGA AAGVFTQPYA VQLVLDAFEA AVTKGWLKEE EVTRESLEGF LSGYGRAFYK
VGDERGERIV LRRKGEKVVE VVRHKEGEVE VVPFRKGQNT WSVEWK
//
