ID A0A0G3EN33_9BACT Unreviewed; 441 AA.
AC A0A0G3EN33;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE SubName: Full=Threonylcarbamoyladenosine tRNA methylthiotransferase MtaB {ECO:0000313|EMBL:AKJ65559.1};
DE EC=2.-.-.- {ECO:0000313|EMBL:AKJ65559.1};
GN Name=mtaB {ECO:0000313|EMBL:AKJ65559.1};
GN ORFNames=L21SP4_02333 {ECO:0000313|EMBL:AKJ65559.1};
OS Kiritimatiella glycovorans.
OC Bacteria; Kiritimatiellota; Kiritimatiellia; Kiritimatiellales;
OC Kiritimatiellaceae; Kiritimatiella.
OX NCBI_TaxID=1307763 {ECO:0000313|EMBL:AKJ65559.1, ECO:0000313|Proteomes:UP000035268};
RN [1] {ECO:0000313|Proteomes:UP000035268}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L21-Fru-AB {ECO:0000313|Proteomes:UP000035268};
RA Spring S., Bunk B., Sproer C., Klenk H.-P.;
RT "Description and complete genome sequence of the first cultured
RT representative of the subdivision 5 of the Verrucomicrobia phylum.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:AKJ65559.1, ECO:0000313|Proteomes:UP000035268}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=L21-Fru-AB {ECO:0000313|EMBL:AKJ65559.1,
RC ECO:0000313|Proteomes:UP000035268};
RX PubMed=27300277; DOI=10.1038/ismej.2016.84;
RA Spring S., Bunk B., Sproer C., Schumann P., Rohde M., Tindall B.J.,
RA Klenk H.P.;
RT "Characterization of the first cultured representative of Verrucomicrobia
RT subdivision 5 indicates the proposal of a novel phylum.";
RL ISME J. 10:2801-2816(2016).
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
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DR EMBL; CP010904; AKJ65559.1; -; Genomic_DNA.
DR RefSeq; WP_052882772.1; NZ_CP010904.1.
DR AlphaFoldDB; A0A0G3EN33; -.
DR STRING; 1307763.L21SP4_02333; -.
DR KEGG; vbl:L21SP4_02333; -.
DR PATRIC; fig|1609981.3.peg.2430; -.
DR OrthoDB; 9805215at2; -.
DR Proteomes; UP000035268; Chromosome.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0035596; F:methylthiotransferase activity; IEA:InterPro.
DR GO; GO:0006400; P:tRNA modification; IEA:InterPro.
DR CDD; cd01335; Radical_SAM; 1.
DR Gene3D; 3.40.50.12160; Methylthiotransferase, N-terminal domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR005839; Methylthiotransferase.
DR InterPro; IPR020612; Methylthiotransferase_CS.
DR InterPro; IPR013848; Methylthiotransferase_N.
DR InterPro; IPR038135; Methylthiotransferase_N_sf.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR NCBIfam; TIGR00089; MiaB/RimO family radical SAM methylthiotransferase; 1.
DR PANTHER; PTHR43020; CDK5 REGULATORY SUBUNIT-ASSOCIATED PROTEIN 1; 1.
DR PANTHER; PTHR43020:SF2; MITOCHONDRIAL TRNA METHYLTHIOTRANSFERASE CDK5RAP1; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR Pfam; PF00919; UPF0004; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDS00029; Radical_SAM; 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51449; MTTASE_N; 1.
DR PROSITE; PS01278; MTTASE_RADICAL; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000035268};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:AKJ65559.1}.
FT DOMAIN 20..131
FT /note="MTTase N-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51449"
FT DOMAIN 143..371
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 441 AA; 47499 MW; 77556E8C445A7C71 CRC64;
MTRTHHDITQ PPASAFGGAL PFRVRVLGCK VNQCEAEEIR GALSARGLRE AGRGESTAVA
VLHSCAVTAE AVRKTRQALR RLAKEEPDRL LLSGCAAGEA LTGELKPDPV RVPAGPGWRE
RLETALDGAG LGRVAGGPVR ASDPRQIRAF LKIQDGCDLG CAYCIVPSLR GTPRDRPPGE
VLAEARELAG RGHREIVVTG ISVGLYGRDG GYDLREVLDP LTRMEDVARV RLSSLHPSEL
TDPLLEFWSR TGKLMPHVHL PLQSGSDAVL AAMGRGYDGA QFRAAVARMR AARENPAITT
DVIVGFPGET EADFARTMEL CRGTGFSRMH VFPFSVRPGT RAAKMGRRVS PDETARRAAR
LRALGAELSA AYHAQFEGRE VRVLLERTVG ESASGLRPAD FRGMTERYVP AECRMRGAAR
GQVATVRVKS SCGEGVTAEE V
//
