ID A0A0G4EG48_VITBC Unreviewed; 1155 AA.
AC A0A0G4EG48;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 37.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=Vbra_11931 {ECO:0000313|EMBL:CEL95501.1};
OS Vitrella brassicaformis (strain CCMP3155).
OC Eukaryota; Sar; Alveolata; Colpodellida; Vitrellaceae; Vitrella.
OX NCBI_TaxID=1169540 {ECO:0000313|EMBL:CEL95501.1, ECO:0000313|Proteomes:UP000041254};
RN [1] {ECO:0000313|EMBL:CEL95501.1, ECO:0000313|Proteomes:UP000041254}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Zhu J., Qi W., Song R.;
RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CDMY01000228; CEL95501.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G4EG48; -.
DR STRING; 1169540.A0A0G4EG48; -.
DR VEuPathDB; CryptoDB:Vbra_11931; -.
DR InParanoid; A0A0G4EG48; -.
DR OMA; HCWRCKT; -.
DR OrthoDB; 227382at2759; -.
DR PhylomeDB; A0A0G4EG48; -.
DR Proteomes; UP000041254; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07960; Anticodon_Ia_Ile_BEm; 1.
DR Gene3D; 1.10.730.20; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR HAMAP; MF_02002; Ile_tRNA_synth_type1; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033708; Anticodon_Ile_BEm.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023585; Ile-tRNA-ligase_type1.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42765:SF1; ISOLEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR42765; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000041254};
KW Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..23
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 24..1155
FT /note="isoleucine--tRNA ligase"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5005187074"
FT DOMAIN 168..806
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 853..1011
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT REGION 51..152
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1155 AA; 128070 MW; EEF083CC7E185B84 CRC64;
MQQWTAAVLP GLFCSVLISL IFSADIGKAD VTERDGCSRR STEGLQHRTI RRIQSPKRSA
DEPPCFIAPA ATRPPPVALR RRVPSRHMLS TAPPPPPAAA AASAAVAEKA PEQTAPTSSE
QKTATAGKKG GRGKGKGKEE ENPFSDSINL PQTTFTQRAN AVTREPELQA FWAEQRVYER
QAESNPGEVF VLHDGPPYAN GELHMGHALN KILKDFINRY QSLRGRKVRY VPGWDCHGLP
IELAVLKSMK SSERQQLTPL DLRRKAASFA LSQVDKQRDS FKRYGVMGEW EKPYLTLDPE
YEAAQIDILG KMLLKGHVYK GKKPVHWSPS SQTALAEAEL EYPDGHVSKS IYASFKVTSP
SDGLARLLPD GGGGGGSGGG DVGVAVWTTT PWTIPGNMAV AVNPELQYAL VRRPDSDAVL
IVAKDLVKEV SKALGALDEL TGEGLEVLAT IKGEDLVDTQ YEHPLYGRVS RVVAGGDYIT
TDTGTGLVHT APGHGQEDFE TGQKYGLEPL SPVDNYGRFT AEAGERFEGL SVLKEGNEAI
IQALDECGSL LKAEDYPHRY PYDWRTKKPT IFRATEQWFI SVDAFREAAL AAIDEVRWIP
EMGKTRIASM TSSRSDWCIS RQRSWGVPIP VFYQAHTNEP LINNSTITHI KEVFRQHGTD
AWWTMDTQQL LPEPYRSEWS LWVRGNDTID VWFDSGSSWA GVVNTRDNLR HDNPVELYLE
GSDQHRGWFQ SSLLTSVAVR GAPPYQTVLT HGFVLDEKGY KMSKSLGNVL SPLTIIEGGS
DKKKQPAYGA DVLRLWVATV DYSSDVLVGG TIIKQVFDAY RKLRNTARFL IGNIHDFDKE
QDGVAYEDLP EVDKWVLGKT ASLIRRVREA YESYQFYRVT QELLAFSNQL LSNFYLDVSK
DRLYISTPND RRRRACQSVI RVVTEAFALL LAPLLPHMAE DIWQNLPHDG EGVRRSGSVF
EAGWPSGAEA SPPFREDKWD LVMTLRDDVN KVIESARTAK LVGANSEAAV HIHCPDPQTT
SWLRELLPDQ SPLLATTTTT TDGRISAVDD LRFLFLTSQV HLADSPGAVE GSCPEFRLSA
EQTGSGCTVG VSKAVGTKCE RCWMWGEDVS EWQGSPRSQD DPPSRHSLCP RCRAAVEQFL
DRRRARAEGG RTNQP
//
