ID A0A0G4EWF6_VITBC Unreviewed; 1405 AA.
AC A0A0G4EWF6;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=beta-galactosidase {ECO:0000256|ARBA:ARBA00012756};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756};
DE AltName: Full=Lactase {ECO:0000256|ARBA:ARBA00032230};
GN ORFNames=Vbra_13717 {ECO:0000313|EMBL:CEM02680.1};
OS Vitrella brassicaformis (strain CCMP3155).
OC Eukaryota; Sar; Alveolata; Colpodellida; Vitrellaceae; Vitrella.
OX NCBI_TaxID=1169540 {ECO:0000313|EMBL:CEM02680.1, ECO:0000313|Proteomes:UP000041254};
RN [1] {ECO:0000313|EMBL:CEM02680.1, ECO:0000313|Proteomes:UP000041254}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Zhu J., Qi W., Song R.;
RL Submitted (NOV-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family.
CC {ECO:0000256|ARBA:ARBA00007401}.
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DR EMBL; CDMY01000333; CEM02680.1; -; Genomic_DNA.
DR STRING; 1169540.A0A0G4EWF6; -.
DR VEuPathDB; CryptoDB:Vbra_13717; -.
DR InParanoid; A0A0G4EWF6; -.
DR OMA; WCEASYI; -.
DR OrthoDB; 276651at2759; -.
DR PhylomeDB; A0A0G4EWF6; -.
DR Proteomes; UP000041254; Unassembled WGS sequence.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:1901575; P:organic substance catabolic process; IEA:UniProt.
DR Gene3D; 2.70.98.10; -; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR004199; B-gal_small/dom_5.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR014718; GH-type_carb-bd.
DR InterPro; IPR006101; Glyco_hydro_2.
DR InterPro; IPR023232; Glyco_hydro_2_AS.
DR InterPro; IPR006103; Glyco_hydro_2_cat.
DR InterPro; IPR023230; Glyco_hydro_2_CS.
DR InterPro; IPR006104; Glyco_hydro_2_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR032312; LacZ_4.
DR PANTHER; PTHR46323; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR46323:SF2; BETA-GALACTOSIDASE; 1.
DR Pfam; PF02929; Bgal_small_N; 1.
DR Pfam; PF02836; Glyco_hydro_2_C; 1.
DR Pfam; PF02837; Glyco_hydro_2_N; 1.
DR Pfam; PF16353; LacZ_4; 1.
DR PRINTS; PR00132; GLHYDRLASE2.
DR SMART; SM01038; Bgal_small_N; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 2.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR PROSITE; PS00719; GLYCOSYL_HYDROL_F2_1; 1.
DR PROSITE; PS00608; GLYCOSYL_HYDROL_F2_2; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000041254};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 81..102
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 122..140
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1010..1389
FT /note="Beta galactosidase small chain/"
FT /evidence="ECO:0000259|SMART:SM01038"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 465..484
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1405 AA; 156795 MW; 5C8F175E063C999F CRC64;
MLESSSPTGR SEELRELLVE SKQATGSPSS PETATTDSPS RECQRQSGRM GCSRTAFSPI
LLSPWFCAVA SLAMGSAILQ YPLWSLAAFT AWLALVWQVR YFEDLQWLVL STGRHWQCFR
RAVFHSAAVS CVMLVIGLHM PLWVKLAAAA VCYLLGVVMT DARRKKDWQN PQVYGKNRLP
MHSPLSYHPS ESAAMALKTY HPRRSMPEEG ARPQQTKCLN GQWRFKLFPS PTALAGNVDW
REFYKRAGSE RDWDRINVPS NWEFEGYSHL IYTNVKYPFV CNPPYVPASD NPTGCYLRDF
SLPSSWAGRR ILLTFGGVSS AYYVWVNGQP VGYAQDSFMP SEFDVTALVR LGDDATNTIA
VQVMRFSDGS YIEDQDHWWT SGIFRDVELF AAPVTAISDY GVQATLDNKY QDGILKARVQ
LAGLEDGRSY SVQFRLYDWE GLTMDPSTPQ DIRLPAMSPI FEHTENVTSG QRTAPDAPTS
TRREETPLLA ETIRDQEHGA CVEWTVRVPS VRPWTAETPS LYFLTIGLVE GGQHEALQWE
GVRVGFRTVE ICDSQLMVNG RPIVVRGVNR HEHDPKAGKA VSLQSAWDDL RLMKQHNINA
VRTSHYPNAP FLYDMCDELG LFVVDEANIE CHGDTPTGYS PIAKYILFNQ SEIPFGRLAS
DPEYRNQWLA RVTRMAQRDK NHPSIIVWSL GNEAGTGPNV RAAYRWLKRY DPTRPVQYEC
DGSPVEVSDI KCPMYPPVEV VEQYGKQQAD RRPMILCEYQ HAMGNSNGSF RDYWEVFSRH
ANLQGGFIWD WVDQGVPKTF NKIECWAYGG DFGDTINDAN FNINGLVLPD RTIKPCMHEV
KYFYQPLAFH LLSAKAGLQG RGGMVTVTIR VTNRHIHQSV TQDMIHLEAA FSTDTAFDFY
DSRAVEYPAV EAGACEEVTI EGRMPPRGKG GCMPDVWLTL RAILTKTTKW ADAGHVVATE
RLTLTDSLRD AVQAVPDPHA LLRAPPAVGA AADADADREL TIEDCGDYVA VRGGDFKLAF
DRSRLGGLIS WVAIPRSHQE WGDLTRKPSR ELAIEYIWDG HGPSVCFVRA PTDNDRGGAT
AGGALDIASQ VLARGLGNIL RIATHKPPGR EGISYASRWE KAKLFELHKP IVHFDAHIRT
LEDPIAGGSM TVAEVSFSQT IAPNHAVDPY FEVDYHYTIF PTGAVHLGCR VCVTPKAAAI
MPPLPRVGLH MMMPPTFRQL VWKGRGPHEN YSDRKSSAFE GVYRSTVDEQ YVPYVKPSEH
GGKCDVQWAA LHTAGSCLLI KAAHFKAFPL QQRDTTSPPA ATAPVLQMNA SRYSVKELMG
AAHTSDIGQG IQVDTNAHIK SPVHLHLDHR HMGVGGEVSW YPCVYEPHLV HPTAPDGSPA
LFEFGLWMLP MHLDSQDDVL AVAAM
//
