ID A0A0G4LCK3_9PEZI Unreviewed; 415 AA.
AC A0A0G4LCK3;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 08-NOV-2023, entry version 22.
DE RecName: Full=Protein phosphatase methylesterase 1 {ECO:0000256|ARBA:ARBA00020672};
DE EC=3.1.1.89 {ECO:0000256|ARBA:ARBA00013111};
DE Flags: Fragment;
GN ORFNames=BN1708_000406 {ECO:0000313|EMBL:CRK19741.1}, HYQ45_013430
GN {ECO:0000313|EMBL:KAG7125026.1};
OS Verticillium longisporum.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Glomerellales; Plectosphaerellaceae; Verticillium.
OX NCBI_TaxID=100787 {ECO:0000313|EMBL:CRK19741.1, ECO:0000313|Proteomes:UP000044602};
RN [1] {ECO:0000313|EMBL:CRK19741.1, ECO:0000313|Proteomes:UP000044602}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=VL1 {ECO:0000313|EMBL:CRK19741.1};
RA Wang D.B., Wang M.;
RL Submitted (MAY-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KAG7125026.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=Vl32 {ECO:0000313|EMBL:KAG7125026.1};
RX PubMed=33955130;
RA Harting R., Starke J., Kusch H., Poggeler S., Maurus I., Schluter R.,
RA Landesfeind M., Bulla I., Nowrousian M., de Jonge R., Stahlhut G.,
RA Hoff K.J., Asshauer K.P., Thurmer A., Stanke M., Daniel R., Morgenstern B.,
RA Thomma B.P.H.J., Kronstad J.W., Braus-Stromeyer S.A., Braus G.H.;
RT "A 20-kb lineage-specific genomic region tames virulence in pathogenic
RT amphidiploid Verticillium longisporum.";
RL Mol. Plant Pathol. 0:0-0(2021).
CC -!- FUNCTION: Demethylates proteins that have been reversibly
CC carboxymethylated. Demethylates the phosphatase PP2A catalytic subunit.
CC {ECO:0000256|ARBA:ARBA00024741}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[phosphatase 2A protein]-C-terminal L-leucine methyl ester +
CC H2O = [phosphatase 2A protein]-C-terminal L-leucine + H(+) +
CC methanol; Xref=Rhea:RHEA:48548, Rhea:RHEA-COMP:12134, Rhea:RHEA-
CC COMP:12135, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17790,
CC ChEBI:CHEBI:90516, ChEBI:CHEBI:90517; EC=3.1.1.89;
CC Evidence={ECO:0000256|ARBA:ARBA00000906};
CC -!- SIMILARITY: Belongs to the AB hydrolase superfamily.
CC {ECO:0000256|ARBA:ARBA00008645}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CVQH01011112; CRK19741.1; -; Genomic_DNA.
DR EMBL; JAEMWZ010000322; KAG7125026.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0G4LCK3; -.
DR STRING; 100787.A0A0G4LCK3; -.
DR OrthoDB; 169198at2759; -.
DR Proteomes; UP000044602; Unassembled WGS sequence.
DR Proteomes; UP000689129; Unassembled WGS sequence.
DR GO; GO:0051723; F:protein methylesterase activity; IEA:InterPro.
DR Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR000073; AB_hydrolase_1.
DR InterPro; IPR016812; PPase_methylesterase_euk.
DR PANTHER; PTHR14189:SF0; PROTEIN PHOSPHATASE METHYLESTERASE 1; 1.
DR PANTHER; PTHR14189; PROTEIN PHOSPHATASE METHYLESTERASE-1 RELATED; 1.
DR Pfam; PF12697; Abhydrolase_6; 1.
DR PIRSF; PIRSF022950; PPase_methylesterase_euk; 1.
DR SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000044602}.
FT DOMAIN 117..384
FT /note="AB hydrolase-1"
FT /evidence="ECO:0000259|Pfam:PF12697"
FT REGION 1..60
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..19
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..36
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 41..59
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 202
FT /evidence="ECO:0000256|PIRSR:PIRSR022950-1"
FT ACT_SITE 230
FT /evidence="ECO:0000256|PIRSR:PIRSR022950-1"
FT ACT_SITE 373
FT /evidence="ECO:0000256|PIRSR:PIRSR022950-1"
FT NON_TER 415
FT /evidence="ECO:0000313|EMBL:CRK19741.1"
SQ SEQUENCE 415 AA; 44594 MW; C823316361EBC3A6 CRC64;
MSDLQRAWAK RKVDAIPEPF DELPDDDADE LSELPDHLDD GDSSSASSAS STGTIIPSPS
QRLFARPGAA LSRPGRSWEP VPWTTYFERE LFLEHDATTT YHAYLTSPSP QGGPLFVAHH
GAGSSALSFA LVAAEIRRAL PHAGFLSLDA RGHGATVTPD QDRPDLSLPT LTADLAAVIA
LTARRMAWPA DALPPMVLVG HSLGGAVITS LAADPPPDLA PRLLGHAVLD VVEGSAMDAL
QSMHAYLSTR PAGFTSIEAA IEWHVRSRTV RNALSARTSV PALLVERVAA AADTEADTGS
RAQTSARPWR WRTDLAATQP FWEGWFTGLS ARFLAARGGK LLLLAGTDRL DTTLTIGQVQ
GRYALQVFPE AGHFIHEDLP EKTAVALVDF FRRNDRGALV LPPKVGDLLR QGKRV
//
