ID A0A0L0DIB0_THETB Unreviewed; 526 AA.
AC A0A0L0DIB0;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 26.
DE RecName: Full=nitrile hydratase {ECO:0000256|ARBA:ARBA00013079};
DE EC=4.2.1.84 {ECO:0000256|ARBA:ARBA00013079};
GN ORFNames=AMSG_07929 {ECO:0000313|EMBL:KNC51841.1};
OS Thecamonas trahens ATCC 50062.
OC Eukaryota; Apusozoa; Apusomonadida; Apusomonadidae; Thecamonas.
OX NCBI_TaxID=461836 {ECO:0000313|EMBL:KNC51841.1, ECO:0000313|Proteomes:UP000054408};
RN [1] {ECO:0000313|EMBL:KNC51841.1, ECO:0000313|Proteomes:UP000054408}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 50062 {ECO:0000313|EMBL:KNC51841.1,
RC ECO:0000313|Proteomes:UP000054408};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Cuomo C., Shea T., Young S.K., Zeng Q., Koehrsen M., Haas B.,
RA Borodovsky M., Guigo R., Alvarado L., Berlin A., Bochicchio J.,
RA Borenstein D., Chapman S., Chen Z., Freedman E., Gellesch M., Goldberg J.,
RA Griggs A., Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D.,
RA Larson L., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shenoy N.,
RA Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J., Yandava C.,
RA Burger G., Gray M.W., Holland P.W.H., King N., Lang F.B.F., Roger A.J.,
RA Ruiz-Trillo I., Lander E., Nusbaum C.;
RT "The Genome Sequence of Thecamonas trahens ATCC 50062.";
RL Submitted (MAY-2010) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an aliphatic amide = a nitrile + H2O; Xref=Rhea:RHEA:12673,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:18379, ChEBI:CHEBI:65285; EC=4.2.1.84;
CC Evidence={ECO:0000256|ARBA:ARBA00001410};
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DR EMBL; GL349470; KNC51841.1; -; Genomic_DNA.
DR RefSeq; XP_013755706.1; XM_013900252.1.
DR AlphaFoldDB; A0A0L0DIB0; -.
DR STRING; 461836.A0A0L0DIB0; -.
DR EnsemblProtists; KNC51841; KNC51841; AMSG_07929.
DR GeneID; 25566742; -.
DR eggNOG; ENOG502S164; Eukaryota.
DR OMA; WYKSRTY; -.
DR OrthoDB; 5489206at2759; -.
DR Proteomes; UP000054408; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 2.30.30.50; -; 1.
DR Gene3D; 3.90.330.10; Nitrile hydratase alpha /Thiocyanate hydrolase gamma; 1.
DR Gene3D; 1.10.472.20; Nitrile hydratase, beta subunit; 1.
DR InterPro; IPR036648; CN_Hdrase_a/SCN_Hdrase_g_sf.
DR InterPro; IPR004232; CN_Hdrtase_a/SCN_Hdrlase_g.
DR InterPro; IPR049054; CN_hydtase_beta-like_N.
DR InterPro; IPR042262; CN_hydtase_beta_C.
DR InterPro; IPR024690; CN_hydtase_beta_dom_C.
DR InterPro; IPR008990; Elect_transpt_acc-like_dom_sf.
DR Pfam; PF02979; NHase_alpha; 1.
DR Pfam; PF02211; NHase_beta_C; 1.
DR Pfam; PF21006; NHase_beta_N; 1.
DR SUPFAM; SSF50090; Electron transport accessory proteins; 1.
DR SUPFAM; SSF56209; Nitrile hydratase alpha chain; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000054408}.
FT DOMAIN 56..152
FT /note="Nitrile hydratase beta subunit-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF21006"
FT DOMAIN 170..262
FT /note="Nitrile hydratase beta subunit"
FT /evidence="ECO:0000259|Pfam:PF02211"
FT DOMAIN 340..525
FT /note="Nitrile hydratase alpha /Thiocyanate hydrolase
FT gamma"
FT /evidence="ECO:0000259|Pfam:PF02979"
FT REGION 282..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 284..305
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 526 AA; 56603 MW; 63A550E953691863 CRC64;
MARLGYLICW DMFAVLRYTT MATVASRAAC AALAASAALT AAAAPGRRAA SGFGRGPHDV
GGAIVSPDEA TAFKAELGKE GAPAALWEVR THALLGLVVG KGLMTVDELR AGIEGLDPVA
YGGLSYYEKW AFSMAQALTA RGTLSPTVLE TAINGDVVRG DEIDSAPVPL SVGQPVRVRR
EEPRPAWSRP HLRTPGYLHG AVGVVDEYWG EFPDPELLAR RIEGPKLPLY SVRFLQAVLW
ELYDGSPHDA TSVEVYHPWL EPISSEALAT AEAAAVASMN KVHGSGRSHH DHAHDHAHAH
DHDHDHDHVH EERCVVESNA IAAESEHALG EGNSPLQRLS GALVEALVDS GLVSRDEVRA
KIESVEKMSE SGSEVGARLV VEAWLDDQFR ELLVADAPAA AEAIGIETSN YGKATGATVL
RVVENTEMVH NLVVCTLCSC YPTTLLGLSP AWYKSRTYRA RAVREPRALL AEFGTRIPDE
VAVKVHDSTA DLRYMVLPLR PEGTEGWSRE ALLGAVTRDA MIGVTR
//
