ID A0A0M0JMF5_9EUKA Unreviewed; 1750 AA.
AC A0A0M0JMF5;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
DE Flags: Fragment;
GN ORFNames=Ctob_007214 {ECO:0000313|EMBL:KOO27675.1};
OS Chrysochromulina tobinii.
OC Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Prymnesiales;
OC Chrysochromulinaceae; Chrysochromulina.
OX NCBI_TaxID=1460289 {ECO:0000313|EMBL:KOO27675.1, ECO:0000313|Proteomes:UP000037460};
RN [1] {ECO:0000313|Proteomes:UP000037460}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CCMP291 {ECO:0000313|Proteomes:UP000037460};
RX PubMed=26397803; DOI=10.1371/journal.pgen.1005469;
RA Hovde B.T., Deodato C.R., Hunsperger H.M., Ryken S.A., Yost W., Jha R.K.,
RA Patterson J., Monnat R.J. Jr., Barlow S.B., Starkenburg S.R.,
RA Cattolico R.A.;
RT "Genome Sequence and Transcriptome Analyses of Chrysochromulina tobin:
RT Metabolic Tools for Enhanced Algal Fitness in the Prominent Order
RT Prymnesiales (Haptophyceae).";
RL PLoS Genet. 11:e1005469-e1005469(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the TOP6A family.
CC {ECO:0000256|ARBA:ARBA00006559}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KOO27675.1}.
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DR EMBL; JWZX01002686; KOO27675.1; -; Genomic_DNA.
DR OrthoDB; 169019at2759; -.
DR Proteomes; UP000037460; Unassembled WGS sequence.
DR GO; GO:0005694; C:chromosome; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003918; F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006259; P:DNA metabolic process; IEA:InterPro.
DR GO; GO:0022414; P:reproductive process; IEA:UniProt.
DR GO; GO:0019953; P:sexual reproduction; IEA:UniProt.
DR CDD; cd00223; TOPRIM_TopoIIB_SPO; 1.
DR Gene3D; 3.40.1360.10; -; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR002815; Spo11/TopoVI_A.
DR InterPro; IPR013049; Spo11/TopoVI_A_N.
DR InterPro; IPR036078; Spo11/TopoVI_A_sf.
DR InterPro; IPR034136; TOPRIM_Topo6A/Spo11.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR10848; MEIOTIC RECOMBINATION PROTEIN SPO11; 1.
DR PANTHER; PTHR10848:SF0; MEIOTIC RECOMBINATION PROTEIN SPO11; 1.
DR Pfam; PF21180; TOP6A-Spo11_Toprim; 1.
DR Pfam; PF04406; TP6A_N; 1.
DR PRINTS; PR01550; TOP6AFAMILY.
DR SUPFAM; SSF56726; DNA topoisomerase IV, alpha subunit; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000037460};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 261..316
FT /note="Spo11/DNA topoisomerase VI subunit A N-terminal"
FT /evidence="ECO:0000259|Pfam:PF04406"
FT DOMAIN 356..529
FT /note="Topoisomerase 6 subunit A/Spo11 TOPRIM"
FT /evidence="ECO:0000259|Pfam:PF21180"
FT REGION 47..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..135
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 540..570
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 600..619
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1482..1503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1691..1724
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 551..566
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1482..1498
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KOO27675.1"
SQ SEQUENCE 1750 AA; 193226 MW; 3D3AD139DAC3764D CRC64;
YAAWQSTHGG AVQQWLQPER QAPSKPWALD HLADAIAADL AHLRKGAQDG ARAQDGARAQ
DGAGARGGGM VRRDSPPRCG SPPRAAQYDA GLCPSGGGGM AAGAPGSALV GGASGGLAAS
RGELSAPSIP ASRPGTSPPV ALIMTAHGAR PATPSHATPT APALSHKQPQ EITGSACPPQ
ALAEVEALAR DALIRIVTES GLGVADLTLL ARPSGALELY DEDDAIVAAA RCAAGAAGEK
DEAADDAIEV RSGTHGGAAR RHLAQLWVVL DAVHATLHDG TKMTQRELWY RLKTTGLFSS
PQQVNERVLD ACAAISFRCG TPCPREALGI TAAPLPGDTE AVRALRFCPR IRARCVLVVE
KDSVFRRLVD DRFVERLPCV LVTACGFPDL ATRMLVRALV DSLKVPAFAL TDYNPHGLCL
MLAYKHGTAR LALEARACCP SLHWIGLRAA DVGAATEEDD ASGLPPDAFQ PFTARDRAVC
AGLRRRPCVA RSPALLAEVG AMEERQCKVE IEALYWRGLG SLSAFLERKI LRAEVEHAAE
ATEATDATEA EANDAPSSSS DDDGGCSSGE SMAIDVRRRS QRAAALGGGA HGGRLLARGD
AAGESAEAEA EEDWLDEGPA EEWDWNMVDV LTPADDEARW LAERRMFPKV GHTLGLVDLP
SNSRPDLAVA IQLLPLFDLR KVGRVSHDDW MRGVKALGVD GLSDPGLWSE LISYFDSGGT
REVHLSQIEW VLPVDPMVGL LLGSLVRAVV HVAEDTWSLK RKSSSQAFAA QSRAVLNMRR
RILEPAMRGW REVISMDRRL ANFSRRLLRH SEWRALRAWC ALTDERRALA RLFKRAMNRE
LTGAFDAWAS SSEEIEATRA RLRSVGARLL NRTLVRAWGT WTGVASARRR MQLVATRALL
AASVRAINQW RAAVAERRRL QTFMRRALHG GLHRAWGRWL ASGDERRQKQ GFGRRWQHRS
LAAAWRAWHA LAEERLRMRG VLGHALNREL SRVWLAWREL WTNWWTVRAR LAIVAKRLLM
KSQLRALNAW VDAHERARHA RELLRRSLLR CMRGSLSRCL ERWLEHLGER RRLQQVGGRL
QHRGLALGWS AWRAAVEEQH AAWRSMQWAA ARVLTPALSR AWSSWLAMLD GTRQRALHAR
RLFAREQLRA FNQWLVLAEA RRRLRRALGG GLVHGALGRA WRQWMDGRNA RRRVQRFMRR
LTNLGVGRVW TTWCDWCVQR AAAISSVRRV GARLRSPAVV GRAWSQWRST CRSIYEQRLR
LQAVAWRALH NGLSRALNAW VEMAADSRRV ASIMFRALHS ALIHAWLVWQ AASQDGRRMR
RAGQRLLSSG VARAWNAWRA LTTARALERR RMVRVLRAAL VRALGHWAHV HRQVRRLRRL
GRRMLHRSLS RAWEQWVSAI AEAERRRQLA RRSLQRILNG LLSRTFGAWA DLARISRAAR
SEVERMRRAV LARMARRIEV MVLQAWRAYV AGVRAMPAAD EAADDEAADV EAADDEADDE
AERRGARRRL ADGVDLSDEV VRLGAQLALT RTELAKVKKS AAWKYELAIV RDELRRAIIA
LARAGSPYLA ATIAALPRVI SEITGEIFGE GSSAFSTPTG TAEPRHRQLT YPLRPSSAAG
WHPRDAVREA LERRDERHVS PPLTAYYTVP AGVGSGLRQF AIPSPVALGP LPSDAWAVEG
VSVSMAVPMA ERPPTRPVGP ASPPLSGPSA SVGAPRGPSP RQRALHEMAM QIGQRLDRES
ETLLGAWESA
//
