ID   A0A0M2H4W2_9MICO        Unreviewed;       500 AA.
AC   A0A0M2H4W2;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   27-MAR-2024, entry version 24.
DE   SubName: Full=3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase {ECO:0000313|EMBL:KJL41505.1};
GN   ORFNames=RS82_02734 {ECO:0000313|EMBL:KJL41505.1};
OS   Microbacterium trichothecenolyticum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=69370 {ECO:0000313|EMBL:KJL41505.1, ECO:0000313|Proteomes:UP000034098};
RN   [1] {ECO:0000313|EMBL:KJL41505.1, ECO:0000313|Proteomes:UP000034098}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 8608 {ECO:0000313|EMBL:KJL41505.1,
RC   ECO:0000313|Proteomes:UP000034098};
RA   Corretto E.;
RT   "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT   metal contaminated environments.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KJL41505.1}.
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DR   EMBL; JYJA01000037; KJL41505.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A0M2H4W2; -.
DR   PATRIC; fig|69370.6.peg.2779; -.
DR   Proteomes; UP000034098; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR   InterPro; IPR021295; DUF2867.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   PANTHER; PTHR12126:SF11; NADH DEHYDROGENASE [UBIQUINONE] 1 ALPHA SUBCOMPLEX SUBUNIT 9, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR12126; NADH-UBIQUINONE OXIDOREDUCTASE 39 KDA SUBUNIT-RELATED; 1.
DR   Pfam; PF11066; DUF2867; 1.
DR   Pfam; PF13460; NAD_binding_10; 1.
DR   SUPFAM; SSF55961; Bet v1-like; 1.
DR   SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE   4: Predicted;
KW   Isomerase {ECO:0000313|EMBL:KJL41505.1};
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000034098};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        467..484
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          15..120
FT                   /note="NAD(P)-binding"
FT                   /evidence="ECO:0000259|Pfam:PF13460"
SQ   SEQUENCE   500 AA;  53921 MW;  40D322BA110640A2 CRC64;
     MPREDGSAPR ALVLGATGYI GGRLVPRLLN AGYEVRVLAR EPARAAAFPW GDRVEIVRGD
     ATDAASVGEA VREVDVLYYL VHSMSGGTTF ADADRRAAQT VSRAAAEGAV GRIVYLGGLH
     PDGVTLSPHL RSRVEVGETF LASGVPTIVL QAGVVIGSGS ASFEMVRHLT EVLPYMPAPK
     WVRNRIQPIA VRDVLHYLLG AARLPADVNR AVDIGGPDVL RYGQMMNGYA VEAGLRQRPI
     AALPVLTPRL ASHWVNLVTP IPRSIARPLV ESLQNECIVK DHAIDALIPP PQGGLTPYRA
     AVRLALRRVE ADAIETSWQD AEVLGAPSDP LPNDPDWAGR AVFTDVRTAH TPASPESLWG
     VIVGIGGENG WYSSPLLWAV RGWMDRLVGG IGLARGRRSK GRLSVGDALD FWRVEALEPG
     RMLRLRAEMK VPGLAWLELQ ASPDGERGGS RYEQRAVFFP SGLAGRLYWL AVLPFHGFIF
     AGMARRITKT AEAAAESAQQ
//