ID   A0A0M2HBJ2_9MICO        Unreviewed;       254 AA.
AC   A0A0M2HBJ2;
DT   11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT   11-NOV-2015, sequence version 1.
DT   24-JAN-2024, entry version 19.
DE   SubName: Full=N(G),N(G)-dimethylarginine dimethylaminohydrolase {ECO:0000313|EMBL:KJL43864.1};
DE            EC=3.5.3.18 {ECO:0000313|EMBL:KJL43864.1};
GN   ORFNames=RS82_01240 {ECO:0000313|EMBL:KJL43864.1};
OS   Microbacterium trichothecenolyticum.
OC   Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC   Microbacterium.
OX   NCBI_TaxID=69370 {ECO:0000313|EMBL:KJL43864.1, ECO:0000313|Proteomes:UP000034098};
RN   [1] {ECO:0000313|EMBL:KJL43864.1, ECO:0000313|Proteomes:UP000034098}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 8608 {ECO:0000313|EMBL:KJL43864.1,
RC   ECO:0000313|Proteomes:UP000034098};
RA   Corretto E.;
RT   "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT   metal contaminated environments.";
RL   Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- SIMILARITY: Belongs to the DDAH family.
CC       {ECO:0000256|ARBA:ARBA00008532}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KJL43864.1}.
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DR   EMBL; JYJA01000029; KJL43864.1; -; Genomic_DNA.
DR   RefSeq; WP_045297661.1; NZ_JYJA01000029.1.
DR   AlphaFoldDB; A0A0M2HBJ2; -.
DR   PATRIC; fig|69370.6.peg.1273; -.
DR   OrthoDB; 3196313at2; -.
DR   Proteomes; UP000034098; Unassembled WGS sequence.
DR   GO; GO:0016403; F:dimethylargininase activity; IEA:UniProtKB-EC.
DR   InterPro; IPR033199; DDAH-like.
DR   PANTHER; PTHR12737:SF9; DIMETHYLARGININASE; 1.
DR   PANTHER; PTHR12737; DIMETHYLARGININE DIMETHYLAMINOHYDROLASE; 1.
DR   SUPFAM; SSF55909; Pentein; 1.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000313|EMBL:KJL43864.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000034098}.
FT   ACT_SITE        161
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-1"
FT   ACT_SITE        246
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-1"
FT   BINDING         18
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-2"
FT   BINDING         60
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-2"
FT   BINDING         65..66
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-2"
FT   BINDING         85
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-2"
FT   BINDING         131
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-2"
FT   BINDING         240
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR633199-2"
SQ   SEQUENCE   254 AA;  27420 MW;  DBE007C4CF6841B4 CRC64;
     MPRLLVRRPS PRLAEGELTH IARVPVDAAL AQRQWESYVD VFRQRGWDVV EVAPADEHAD
     GVFIEDAVVM FGDLAVLTSP GAESRRGEIA STRSTLEGAG IPYRAIELPG TLDGGDVLKI
     GRTVYVGRTL RTNDEGITQL RDLLAPRGWS VVGVPVSKVL HLKSGVTALP DGTVVGFEPL
     VDDPAAYPDF LAVPEEHGTA VVVLDDATVL MSADAPETAE LYRSRGLEVI TTPITEFEKL
     EGCVTCLSVR VRDV
//