ID A0A0M2HJA3_9MICO Unreviewed; 1040 AA.
AC A0A0M2HJA3;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE SubName: Full=Minor extracellular protease vpr {ECO:0000313|EMBL:KJL44419.1};
DE EC=3.4.21.- {ECO:0000313|EMBL:KJL44419.1};
GN Name=vpr_1 {ECO:0000313|EMBL:KJL44419.1};
GN ORFNames=RS82_00813 {ECO:0000313|EMBL:KJL44419.1};
OS Microbacterium trichothecenolyticum.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=69370 {ECO:0000313|EMBL:KJL44419.1, ECO:0000313|Proteomes:UP000034098};
RN [1] {ECO:0000313|EMBL:KJL44419.1, ECO:0000313|Proteomes:UP000034098}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 8608 {ECO:0000313|EMBL:KJL44419.1,
RC ECO:0000313|Proteomes:UP000034098};
RA Corretto E.;
RT "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT metal contaminated environments.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240,
CC ECO:0000256|RuleBase:RU003355}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJL44419.1}.
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DR EMBL; JYJA01000026; KJL44419.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M2HJA3; -.
DR PATRIC; fig|69370.6.peg.837; -.
DR Proteomes; UP000034098; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProt.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd07474; Peptidases_S8_subtilisin_Vpr-like; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 2.
DR InterPro; IPR010435; Fn3_5.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034213; S8_Vpr-like.
DR PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR Pfam; PF06280; fn3_5; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF82171; DPP6 N-terminal domain-like; 1.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000034098};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..24
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 25..1040
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5017983190"
FT DOMAIN 173..630
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 440..529
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT DOMAIN 679..777
FT /note="Fn3-like"
FT /evidence="ECO:0000259|Pfam:PF06280"
FT REGION 22..51
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 182
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 260
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 594
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 1040 AA; 105110 MW; 918518395BD16811 CRC64;
MLRRSLAVVS ALALAIATPT AAMAAAPDDD PSSRFEKSDT KGPVGTTARP LSVGADGRVT
VIVEMAGDPV AVAQAKKGSD LSAAERKAIK SSLKKNQDAI TGTIKAKDGR IEATMQSAYN
GIQMNIPADQ VDAVAALPNV VAVHAVKTYT LDNAVSVPFL GVPQVWANTG FTGENIKVAV
IDTGIDYTHA NFGGVGTPEA YAAANAAEAQ PADPAQFGPD APRIKGGWDF VGDAYNADPA
SDDYDPVPKP DANPLDCNGH GSHVAGTAAG SGVTADGETY AGPYDESTAS AEWTIGPGVA
PQADLYALRV FGCGGSTDIV VPAIDWAVDN GMDVINMSLG SPFGRGGDPD EVAASNAIGA
GVVVVKSAGN EGPSPYLAGN GNGVVSVAAV DSTATFPGAL ITVDGVAVPA INANGAVLTG
LPAMTVVRLT DNPATPDENE ALGCSVAAYN YAGVTVGGNQ LAVSTRGVCA RVAKAIFAQQ
AGAAAAVMIN TSNDYPPFEG EITENPDTGD DYTVTIPFLG VRMSDGAKLV DDAEATVAPA
PLENPAFRGY ASFSSSGPRS GDSALGVDVA APGVSIVSTG VGTGNGPATI SGTSMAAPHV
AGVAALAVEA HPAWSAAEVA SVLVSTADPD KVVGQNLVRG GVGLVDTAQA VSQQVTATGD
SFRTDDGKLR ESALSFGFQE TAKTFQGKKT VTLTNYGSQP VTYSVGTEAT AQSEAATVTA
SPAQVTVKPG RSEKVTVDLT APATAVGSSG AGGFAFYEFS GDVVLTSASD TLRVPYLLVP
RATTDVSASG DLKVTKKNKP VDGDVSLTLK NNRGAFAGDA DFYTWGLEDR KDAPKSFTDT
GYDLRAAGVQ SFPWDDAGDQ LLVFAVNTHN RWSNAAANEF DVVIDTNGDG EPDWIVFSAD
GGLVTAGDAN GISQVFVYEI ATGEVSAAGF NASAPTDSST ILLPVVASDL GITSGAFSYT
VESFSSVNDG SDAIDGTATY DPWAPALTNG QFESVAVGGK VDVPVTVNGA AFSAQKPLGV
MVVVMDNAAG PGEALLVKAK
//
