ID A0A0M2HRB3_9MICO Unreviewed; 1187 AA.
AC A0A0M2HRB3;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 24-JAN-2024, entry version 34.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969,
GN ECO:0000313|EMBL:KJL47037.1};
GN ORFNames=RS84_03683 {ECO:0000313|EMBL:KJL47037.1};
OS Microbacterium hydrocarbonoxydans.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microbacterium.
OX NCBI_TaxID=273678 {ECO:0000313|EMBL:KJL47037.1, ECO:0000313|Proteomes:UP000033900};
RN [1] {ECO:0000313|EMBL:KJL47037.1, ECO:0000313|Proteomes:UP000033900}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SA35 {ECO:0000313|EMBL:KJL47037.1,
RC ECO:0000313|Proteomes:UP000033900};
RA Corretto E.;
RT "Draft genome sequences of ten Microbacterium spp. with emphasis on heavy
RT metal contaminated environments.";
RL Submitted (FEB-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KJL47037.1}.
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DR EMBL; JYJB01000010; KJL47037.1; -; Genomic_DNA.
DR RefSeq; WP_045259117.1; NZ_JYJB01000010.1.
DR AlphaFoldDB; A0A0M2HRB3; -.
DR STRING; 273678.RS84_03683; -.
DR PATRIC; fig|273678.4.peg.3678; -.
DR Proteomes; UP000033900; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000033900}.
FT DOMAIN 645..806
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 831..981
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1187 AA; 128626 MW; B08C43C0E1E4B516 CRC64;
MTVPGILRAL EEASLYRDAL SWAHTDADLG LVDGLDAPAL AGLLGKRAAA GHPAALLAVV
PTGRRAESLA QALAAYISDA EVLTFPAWET LPHERLSPSP DTVGQRLQTL RRVAGWTGDH
PLIVVASVRA ALQPIAGNLG EIEPVVLKAG GRGYDLDGVA EQLVERAYSR VDMVSRRGEF
AVRGGILDVF PPISEHPYRV EFFGDEVDQI RAFSVADQRS LPGEVELVDL APSRELLLTQ
DVRDNARALK DAFPAIAGML EKMAEGIPVE GMESLLPAVA GPLKSLVEYM PAGSATAVVD
PERASARAIT LGDTNREFLD AAWSAATSGA SAPIDLGAGD FLTIAQLREV VRDRGGVWWR
LSPFAMGGED AETVDAAVIP SFHGNVDGAI SFVDAKIQDG WRVVVIATGT GLVDRARDVL
SDRGIAARIV DDLLDVPDAG VATLTAGSVE SGFQIADAKL AVLTDNEFYG RTIGGDQRVV
KKLASKRKNV VDPLQLKQGD FVVHATHGIG RFVEMTKREV STGGRNATKS VRDYLVLEYA
PSKRGYPGDK LFVPTDQLDL LSKYVGGEAP TLSKMGGSDW AQAKGKARKA VRDIAVELVK
LYSARMSAKG HAFGPDTPWQ RELEEAFPFA ETQDQLQTIE EIKADMERPI PMDRLLSGDV
GFGKTEVAVR AAFKAIQDGK QVAMLVPTTL LVKQHLETFT ERFAGFPVKV RPLSRFQTDK
EARLTLQGLL DGTVDMVIGT HRILTDQVMF KDLGLMIIDE EQRFGVEHKD ALKKMKTNVD
ILAMSATPIP RTLEMAVTGI REMSTLQTPP EDRHPILSFV GPRSDKQIAA AIRREILREG
QVFFVHNRVQ SIQRVAAEIA ELVPEARIAV AHGQLGEHQL EQVVDDFWER KFDVLVCTTI
VETGLDISNA NTIIIDRADK YGLSQLHQLR GRVGRGRERA YAYFLYDDAK PLSETAADRL
QTIAVNNDLG SGMQVALKDL ELRGAGNMLG AEQAGHIAGV GFDLYLRMIG EAVSTFRGEE
VESGQELRLE LPLDARIPED YIDSERLRLE AYQKLSAASA ATAKDDAIDL VIEELTDRYG
ALPSEAEGLI AVARLRRRAA RAGLTDVVTM GSNLRIAPAR LEDSIKVRMQ RLYPKAKLVG
GGEALVVPMP TVPAAVGVGV EPLAGAALLE WVAQLFTALF PEPAKAE
//
