ID A0A0M4MBG7_9ACTN Unreviewed; 714 AA.
AC A0A0M4MBG7;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Neutral ceramidase {ECO:0000256|RuleBase:RU366019};
DE EC=3.5.1.23 {ECO:0000256|RuleBase:RU366019};
GN ORFNames=AL705_01465 {ECO:0000313|EMBL:ALE18599.1};
OS Lawsonella clevelandensis.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Lawsonellaceae;
OC Lawsonella.
OX NCBI_TaxID=1528099 {ECO:0000313|EMBL:ALE18599.1, ECO:0000313|Proteomes:UP000068137};
RN [1] {ECO:0000313|EMBL:ALE18599.1, ECO:0000313|Proteomes:UP000068137}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=X1698 {ECO:0000313|EMBL:ALE18599.1,
RC ECO:0000313|Proteomes:UP000068137};
RX PubMed=26659691;
RA Nicholson A.C., Bell M., Humrighouse B.W., McQuiston J.R.;
RT "Complete Genome Sequences for Two Strains of a Novel Fastidious, Partially
RT Acid-Fast, Gram-Positive Corynebacterineae Bacterium, Derived from Human
RT Clinical Samples.";
RL Genome Announc. 3:e01462-15(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an N-acylsphing-4-enine + H2O = a fatty acid + sphing-4-enine;
CC Xref=Rhea:RHEA:20856, ChEBI:CHEBI:15377, ChEBI:CHEBI:28868,
CC ChEBI:CHEBI:52639, ChEBI:CHEBI:57756; EC=3.5.1.23;
CC Evidence={ECO:0000256|RuleBase:RU366019};
CC -!- SIMILARITY: Belongs to the neutral ceramidase family.
CC {ECO:0000256|ARBA:ARBA00009835, ECO:0000256|RuleBase:RU366019}.
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DR EMBL; CP012390; ALE18599.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M4MBG7; -.
DR STRING; 1528099.AL705_01465; -.
DR KEGG; cbq:AL705_01465; -.
DR PATRIC; fig|1562462.4.peg.301; -.
DR Proteomes; UP000068137; Chromosome.
DR GO; GO:0102121; F:ceramidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0017040; F:N-acylsphingosine amidohydrolase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0046514; P:ceramide catabolic process; IEA:InterPro.
DR Gene3D; 2.60.40.2300; Neutral/alkaline non-lysosomal ceramidase, C-terminal domain; 1.
DR InterPro; IPR006823; Ceramidase_alk.
DR InterPro; IPR038445; NCDase_C_sf.
DR InterPro; IPR031331; NEUT/ALK_ceramidase_C.
DR InterPro; IPR031329; NEUT/ALK_ceramidase_N.
DR InterPro; IPR006311; TAT_signal.
DR PANTHER; PTHR12670; CERAMIDASE; 1.
DR PANTHER; PTHR12670:SF1; NEUTRAL CERAMIDASE; 1.
DR Pfam; PF04734; Ceramidase_alk; 1.
DR Pfam; PF17048; Ceramidse_alk_C; 1.
DR PROSITE; PS51318; TAT; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU366019};
KW Lipid metabolism {ECO:0000256|RuleBase:RU366019};
KW Reference proteome {ECO:0000313|Proteomes:UP000068137};
KW Sphingolipid metabolism {ECO:0000256|RuleBase:RU366019}.
FT DOMAIN 51..551
FT /note="Neutral/alkaline non-lysosomal ceramidase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF04734"
FT DOMAIN 553..713
FT /note="Neutral/alkaline non-lysosomal ceramidase C-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF17048"
SQ SEQUENCE 714 AA; 79415 MW; 9F4D417EE28E9895 CRC64;
MEINRRSLLK GMAATGVAAA VATGTTQSTG TPFAQAAKRH KITGPADGKG YSVGVGISSM
TGAVAGQGMM GYSEPEQVAR GLRSPYYARA FIFVDNKTKE RLVYVNVDMA CFFEALHQEV
IKHCAKKYGK LYPESHLCIT ATHNHNSCGG TSHNMAYNTA TSGFMENSFK EEVAGICEAI
DKAHHAIRPA TLLLGHAELH NASANRSTVA FERNPDSDKS YFPERIDPQV RVLRIQQAGK
DVGAITWFAT HGTSLTDANF YIAPDNKGLA SYMWEHDEKG VRYLRGHHNF VAAFSQTNAG
DMTPNLGIKQ MKPTGPDGTN FVKNNVIIAK RQYDACKKAF ESATPISGAR LDVRYMYVDM
AHQVIDGKYT HDGKRWRTSP AIMGGSGAAC SMEDNVRCPT WDIPFIREGW QFPDAGPKIK
SIQDAMKKMG IKKVPELPRE DMLNQYPKVP LLPLGYLPPT PWNPQIVPAQ IIRLCDQLTM
CTNSSECTIV AALRVRRQVA KTLGIHLEDV IYQGYTNSYN QYITTPEEYM ASQYEAGETQ
YGIETLGAWI QAFDKLAQAM RDGKPVAHGP SPLKHEWFHP TWLAAVPPDT CPKGKKYGDV
LVPPQRGYHK GQTAQVDFVG AHPNNRIRRN STYTKVEQKK GGSWVCVYDD SDYETMFHWE
RPKDSKTESI TRVIWNIPTW QETGMYRLVQ LGESRDKNGK ITSYVGTSPA FRVS
//
