ID A0A0N1DTB9_9FLAO Unreviewed; 627 AA.
AC A0A0N1DTB9;
DT 09-DEC-2015, integrated into UniProtKB/TrEMBL.
DT 09-DEC-2015, sequence version 1.
DT 24-JAN-2024, entry version 25.
DE RecName: Full=Beta-galactosidase {ECO:0000256|RuleBase:RU000675};
DE EC=3.2.1.23 {ECO:0000256|RuleBase:RU000675};
GN ORFNames=AMQ68_18185 {ECO:0000313|EMBL:KPH11350.1};
OS Chryseobacterium sp. ERMR1:04.
OC Bacteria; Bacteroidota; Flavobacteriia; Flavobacteriales; Weeksellaceae;
OC Chryseobacterium group; Chryseobacterium.
OX NCBI_TaxID=1705393 {ECO:0000313|EMBL:KPH11350.1, ECO:0000313|Proteomes:UP000037945};
RN [1] {ECO:0000313|EMBL:KPH11350.1, ECO:0000313|Proteomes:UP000037945}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ERMR1:04 {ECO:0000313|EMBL:KPH11350.1,
RC ECO:0000313|Proteomes:UP000037945};
RX PubMed=26543128;
RA Kumar R., Singh D., Swarnkar M.K., Singh A.K., Kumar S.;
RT "Genome Assembly of Chryseobacterium polytrichastri ERMR1:04, a
RT Psychrotolerant Bacterium with Cold Active Proteases, Isolated from East
RT Rathong Glacier in India.";
RL Genome Announc. 3:e01305-15(2015).
RN [2] {ECO:0000313|Proteomes:UP000037945}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ERMR1:04 {ECO:0000313|Proteomes:UP000037945};
RA Swarnkar M.K., Kumar R., Singh A.K., Singh D.;
RT "Genome Sequencing of Chryseobacterium sp. ERMR1:04 isolated from Glacier
RT Moraine Ridge soil.";
RL Submitted (AUG-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|RuleBase:RU000675};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 35 family.
CC {ECO:0000256|ARBA:ARBA00009809, ECO:0000256|RuleBase:RU003679}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPH11350.1}.
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DR EMBL; LIRF01000008; KPH11350.1; -; Genomic_DNA.
DR RefSeq; WP_054512028.1; NZ_LIRF01000008.1.
DR AlphaFoldDB; A0A0N1DTB9; -.
DR STRING; 1705393.AMQ68_18185; -.
DR PATRIC; fig|1705393.3.peg.3839; -.
DR OrthoDB; 703126at2; -.
DR Proteomes; UP000037945; Unassembled WGS sequence.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 2.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR InterPro; IPR026283; B-gal_1-like.
DR InterPro; IPR048912; BetaGal1-like_ABD1.
DR InterPro; IPR048913; BetaGal_gal-bd.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR031330; Gly_Hdrlase_35_cat.
DR InterPro; IPR019801; Glyco_hydro_35_CS.
DR InterPro; IPR001944; Glycoside_Hdrlase_35.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR23421:SF165; BETA-GALACTOSIDASE; 1.
DR PANTHER; PTHR23421; BETA-GALACTOSIDASE RELATED; 1.
DR Pfam; PF21317; BetaGal_ABD_1; 1.
DR Pfam; PF21467; BetaGal_gal-bd; 1.
DR Pfam; PF01301; Glyco_hydro_35; 1.
DR PIRSF; PIRSF006336; B-gal; 1.
DR PRINTS; PR00742; GLHYDRLASE35.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 2.
DR PROSITE; PS01182; GLYCOSYL_HYDROL_F35; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU000675};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU000675}.
FT DOMAIN 36..359
FT /note="Glycoside hydrolase 35 catalytic"
FT /evidence="ECO:0000259|Pfam:PF01301"
FT DOMAIN 403..513
FT /note="Beta-galactosidase 1-like first all-beta"
FT /evidence="ECO:0000259|Pfam:PF21317"
FT DOMAIN 540..598
FT /note="Beta-galactosidase galactose-binding"
FT /evidence="ECO:0000259|Pfam:PF21467"
FT ACT_SITE 183
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
FT ACT_SITE 267
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR006336-1"
SQ SEQUENCE 627 AA; 72016 MW; AB0EE95B819CAD20 CRC64;
MKTKLKYVWT FLLILTTVSA FSQKINKNFE IKDGHFLLNG KPFSIYSGEL HYPRVPSQYW
KHRLQMMKAM GLNTVTTYVF WNYHEETPGK WNFSGEKDLQ QFIKTAQEVG LYVIIRPGPY
VCAEWEFGGY PWWLQNIKGL KIREDNDQFL IETKKYITQL YNQVKDLQIT NGGPVIMVQA
ENEFGSFVSQ RKDIPLASHR TYNAKIVKQL KDAGFSVPLF TSDGSWLFEG GSVVGALPTA
NGEDNVENLK KIVNQYNNNQ GPYMVAEFYP GWLGHWAEEF PRVDATTVAR QTNKYLKNDV
SFNYYMVHGG TNFGFTNGAN YDKNHDIQPD LTSYDYDAPI TEAGWRTPKY DSLRAVISKH
TKAKLPEIPA PIKVIEIKDI QLNKVYNLFN YAAGQEIIKS DKPQSFEELN QGHGYVLYRR
HFNQPIKGIL DLKGLRDYAT IYINGEKVGE LNRYYNQYTM PIDIPFNAKL EILVENWGRI
NYGERINENT KGIISAVKIG DTEITGNWEM TKLPFLNDFA STIKAKPVDE GKQVKLKDVP
TVYQGEFDLN EVGDTFIDMQ KWGKGVIFVN GKNIGRFWKQ GPQQTLYIPG VWLKKGKNEV
IIFDQLNETV QTKVSTVKTP ILDQLAK
//
