ID A0A0P5SLC7_9CRUS Unreviewed; 926 AA.
AC A0A0P5SLC7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=APZ42_029339 {ECO:0000313|EMBL:KZS06850.1};
OS Daphnia magna.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=35525 {ECO:0000313|EMBL:JAL65389.1};
RN [1] {ECO:0000313|EMBL:JAL65389.1}
RP NUCLEOTIDE SEQUENCE.
RA Gilbert D., Podicheti R., Orsini L., Colbourne J., Pfrender M.;
RT "Daphnia magna gene sets from two clonal populations assembled and
RT annotated with EvidentialGene.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:JAL65389.1}
RP NUCLEOTIDE SEQUENCE.
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KZS06850.1, ECO:0000313|Proteomes:UP000076858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Xinb3 {ECO:0000313|EMBL:KZS06850.1,
RC ECO:0000313|Proteomes:UP000076858};
RC TISSUE=Complete organism {ECO:0000313|EMBL:KZS06850.1};
RA Gilbert D.G., Choi J.-H., Mockaitis K., Colbourne J., Pfrender M.;
RT "EvidentialGene: Evidence-directed Construction of Genes on Genomes.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; GDIP01138325; JAL65389.1; -; Transcribed_RNA.
DR EMBL; LRGB01002580; KZS06850.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0P5SLC7; -.
DR STRING; 35525.A0A0P5SLC7; -.
DR Proteomes; UP000076858; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:UniProt.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR017868; Filamin/ABP280_repeat-like.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR PANTHER; PTHR11254:SF340; APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF81296; E set domains; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50194; FILAMIN_REPEAT; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000076858};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT TRANSMEM 7..26
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT REPEAT 214..301
FT /note="Filamin"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00087"
FT DOMAIN 577..926
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 893
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 926 AA; 104841 MW; FD1578DEFB3AA84F CRC64;
MPLDYRCIGW WILVAYIVAV IVYFGANKNT RNNLDNQFFK CSENQGTNKE ETNVLCMWLQ
DSKLLDLVGR LPELGVKNLE DVYKLEDLKI QTLLGPDDAK RWLLARSTLP DDIQKLPVLK
EKVLIGSAHQ AAWKWVKEVA VVLLILWGVS RLAISCGWTR AFCPVSLNCT FFHGIIDFFI
DADIRQTFLQ WLSGNRALAK SARIVFYWDE PLMVGSTVSF TVEFFQRWSR RPYPVSDADS
IRINIRQGAN KIAAAVQIGG SLETDANLVR ATFTVRRAGQ YHIDVNLGSI PIRGSPFVKY
VRPGPPDPSK TTLVRPTSMV VCTARSPHQV LLEPRDEYGN YCSWNHDAPE QQKALDAFSC
EAYSITSSDV VRPLIQWLWV ELMHRLIINV TFNEEGIYGV RLKLDDSVIS KGEFNMIALS
TSDTIQVEKA LGARTATYET KLLSINGEKW TKNKKVFCAI SPKQIALKEY ILGIIPKRLA
TFRLCPATKV TFLKSTNKQE FPVLTIEDGA QFVVELSSKE AHLMAASFAH YLLKNIGGSE
TFTDKQGFFY QEIRKFHQKK PHEKLLLRVE RDKILESSMK LTKSFSITDW CRNFEISFIG
EQGLDWGGLR REWIELLCVS IFDRRQSGMF TSFSDDGQAL VHPNPTIPAS CKLKHYEFAG
KLVGKCLYES SLGATYRQLI KARFTRSFLA QLIGLRINYK YFEHDDPPLF LSKIKYIEEN
DVSDMELVFA EEEYDEQGKL LRTVELIPGG ARIPVDNANK HRYLDALAQH RLVNSVRDQV
DAFTRGLAEL VPDNLLSIFD ENELELLVCG TSDYSVAEMK AHHAAIGSSP EFQKVLCWFW
TAVTNFGTEE MSRLLQFTTG SSQLPPGGLA ELSPKLQIAA SPCFGTLPTA HTCFNQLCLP
DYENYEQFEK ALLIAINEGS EGFGLV
//
