ID A0A0P7DE88_9GAMM Unreviewed; 440 AA.
AC A0A0P7DE88;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|ARBA:ARBA00019077, ECO:0000256|RuleBase:RU365103};
DE Short=Kdo transferase {ECO:0000256|RuleBase:RU365103};
DE EC=2.4.99.12 {ECO:0000256|ARBA:ARBA00012621, ECO:0000256|RuleBase:RU365103};
DE AltName: Full=Lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase {ECO:0000256|ARBA:ARBA00031445, ECO:0000256|RuleBase:RU365103};
GN ORFNames=AOG28_10565 {ECO:0000313|EMBL:KPM78298.1};
OS Cobetia sp. UCD-24C.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Cobetia.
OX NCBI_TaxID=1716176 {ECO:0000313|EMBL:KPM78298.1, ECO:0000313|Proteomes:UP000050261};
RN [1] {ECO:0000313|EMBL:KPM78298.1, ECO:0000313|Proteomes:UP000050261}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UCD-24C {ECO:0000313|EMBL:KPM78298.1,
RC ECO:0000313|Proteomes:UP000050261};
RA Krusor M., Coil D.A., Lang J.M., Eisen J.A., Alexiev A.;
RT "Draft Genome of Cobetia sp. UCD-24C.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Involved in lipopolysaccharide (LPS) biosynthesis. Catalyzes
CC the transfer of 3-deoxy-D-manno-octulosonate (Kdo) residue(s) from CMP-
CC Kdo to lipid IV(A), the tetraacyldisaccharide-1,4'-bisphosphate
CC precursor of lipid A. {ECO:0000256|RuleBase:RU365103}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=CMP-3-deoxy-beta-D-manno-octulosonate + lipid IVA (E. coli) =
CC alpha-Kdo-(2->6)-lipid IVA (E. coli) + CMP + H(+);
CC Xref=Rhea:RHEA:28066, ChEBI:CHEBI:15378, ChEBI:CHEBI:58603,
CC ChEBI:CHEBI:60364, ChEBI:CHEBI:60377, ChEBI:CHEBI:85987;
CC EC=2.4.99.12; Evidence={ECO:0000256|ARBA:ARBA00034406,
CC ECO:0000256|RuleBase:RU365103};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; LPS core biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004713, ECO:0000256|RuleBase:RU365103}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU365103}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC {ECO:0000256|RuleBase:RU365103}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPM78298.1}.
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DR EMBL; LJTD01000006; KPM78298.1; -; Genomic_DNA.
DR RefSeq; WP_054556385.1; NZ_LJTD01000006.1.
DR AlphaFoldDB; A0A0P7DE88; -.
DR STRING; 1716176.AOG28_10565; -.
DR UniPathway; UPA00958; -.
DR Proteomes; UP000050261; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0043842; F:Kdo transferase activity; IEA:UniProtKB-EC.
DR GO; GO:0009244; P:lipopolysaccharide core region biosynthetic process; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.11720; 3-Deoxy-D-manno-octulosonic-acid transferase, N-terminal domain; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 1.
DR InterPro; IPR007507; Glycos_transf_N.
DR InterPro; IPR038107; Glycos_transf_N_sf.
DR InterPro; IPR039901; Kdotransferase.
DR PANTHER; PTHR42755:SF1; 3-DEOXY-D-MANNO-OCTULOSONIC ACID TRANSFERASE, MITOCHONDRIAL-RELATED; 1.
DR PANTHER; PTHR42755; 3-DEOXY-MANNO-OCTULOSONATE CYTIDYLYLTRANSFERASE; 1.
DR Pfam; PF04413; Glycos_transf_N; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|RuleBase:RU365103};
KW Lipopolysaccharide biosynthesis {ECO:0000256|RuleBase:RU365103};
KW Membrane {ECO:0000256|RuleBase:RU365103};
KW Reference proteome {ECO:0000313|Proteomes:UP000050261};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU365103}.
FT DOMAIN 35..221
FT /note="3-deoxy-D-manno-octulosonic-acid transferase N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF04413"
FT ACT_SITE 63
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-1"
FT SITE 141
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
FT SITE 219
FT /note="Transition state stabilizer"
FT /evidence="ECO:0000256|PIRSR:PIRSR639901-2"
SQ SEQUENCE 440 AA; 47364 MW; EBD9B31EB0594D4E CRC64;
MSLARGLYST ALTLLSPLIW QRVWREHDAQ HPRLERLGII PSTPNTTPDI ERPIWLHAAS
LGEVVMVASL VEALRQRYPR RPLVLTTMTA TGAAQAARLL HTPQGSSGLA PARHHYLPLD
FPLSARRFVR RLRPALAIMV ETELWPNLMA ACARDDIALC VINGRLSPRA FQRYRRIGAL
MRDSLAHVSG LSAKSQQDLE RFMALGLPPA RGTAVGSLKF ELDIAEQVIA DGQVLRQSFA
QRPVWIAAST HDGEDGQILA AHAAVRDQHP QALLLLVPRH PQRFEAVAAQ AAQWCQSRGE
SSLRRSAMTE SGTEVADAAS VLVGDSLGEL LTLYAASDIA FIGGSLVPIG GHNLLEPAAL
GLPLLSGPSL DNFSEIAEVM AAEGALQVVA DDAELAEAVN ALFSDQAAQQ VAGQAASQVV
ERNRGALSGT LAMLEHHLPL
//
