ID A0A0P9A6G7_9PROT Unreviewed; 838 AA.
AC A0A0P9A6G7;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000256|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000256|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000256|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000256|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000256|HAMAP-Rule:MF_00049,
GN ECO:0000313|EMBL:KPU81965.1};
GN ORFNames=JI56_03910 {ECO:0000313|EMBL:KPU81965.1};
OS SAR11 cluster bacterium PRT-SC02.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Candidatus Pelagibacterales;
OC Candidatus Pelagibacteraceae.
OX NCBI_TaxID=1527298 {ECO:0000313|EMBL:KPU81965.1, ECO:0000313|Proteomes:UP000050662};
RN [1] {ECO:0000313|EMBL:KPU81965.1, ECO:0000313|Proteomes:UP000050662}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=PRT-SC02 {ECO:0000313|EMBL:KPU81965.1};
RA Leon Zayas R.I., Novotny M., Podell S., Shepard C.M., Berkenpas E.,
RA Nikolenko S., Pevzner P., Lasken R.S., Bartlett D.H.;
RT "Microbial Metabolic Properties Below 8,000 Meters Depth Within the Puerto
RT Rico Trench Inferred From Single Cell Genomes.";
RL Submitted (AUG-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000256|ARBA:ARBA00001372, ECO:0000256|HAMAP-
CC Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|HAMAP-Rule:MF_00049,
CC ECO:0000256|RuleBase:RU363035}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|HAMAP-Rule:MF_00049}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPU81965.1}.
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DR EMBL; JPUP01000096; KPU81965.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0P9A6G7; -.
DR PATRIC; fig|1527298.3.peg.768; -.
DR Proteomes; UP000050662; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00812; LeuRS_core; 1.
DR Gene3D; 2.20.28.290; -; 1.
DR Gene3D; 3.10.20.590; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00396; leuS_bact; 1.
DR PANTHER; PTHR43740:SF2; LEUCINE--TRNA LIGASE, MITOCHONDRIAL; 1.
DR PANTHER; PTHR43740; LEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00049};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00049};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00049};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00049};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00049};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00049}; Reference proteome {ECO:0000313|Proteomes:UP000050662}.
FT DOMAIN 37..167
FT /note="Methionyl/Leucyl tRNA synthetase"
FT /evidence="ECO:0000259|Pfam:PF09334"
FT DOMAIN 218..398
FT /note="Leucyl-tRNA synthetase editing"
FT /evidence="ECO:0000259|Pfam:PF13603"
FT DOMAIN 608..647
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 684..799
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
FT MOTIF 38..48
FT /note="'HIGH' region"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00049"
FT BINDING 611
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00049"
SQ SEQUENCE 838 AA; 98043 MW; A63042B69BABFBFC CRC64;
MERIVFNKIE KKWQDIWSEK KVKKKKNQKK FYCLEMFPYP SGKIHMGHVR NYTIGDVIAR
HKIMKGFDVM HPMGWDAFGL PAENAAKENN LNPKEWTFQN IETMKKQLQM LGFSIDWERE
ISTCKEDYYK QQQSLFIDLY KNDFVGRKET YVNWDPVEKT VLANEQVIDG KGWRSNQPVE
RKKLNQWFFK ITKFSEDLLN DMKTLDNWPN KVKLMQSNWI GKSVGCEIDF QLSNSKEKIK
VFTTRPDTIF GATFLSISVD HPICKIIKSQ KEFQNFKLKC LKTGTTEEAI ANAEKEGFKT
EFSAINPFNK KEIPVYVSNF VLMDYGTGAI FGCPAHDQRD LDFAIKYDLE VIQVVKENDL
IDFKKTGTAF TDHGIMVNSS MLNGLSTSDA KIKIIKEVEK NKIGKKKISY RLRDWGVSRQ
RYWGCPIPIV YDKKGKIHVL DKSELPVKLP EDVDFNQPGN PLDKHKNWKY YKNSKGEEFI
RETDTLDTFV DSSWYFLRFC SPQNNQEPFN LSEVNYWMPV DQYIGGVEHA ILHLLYSRFF
VKALKKCGYK IEFDEPFKNL FTQGMITHET YKDENGKWLS PDEIEKVGDK LAKKKKDKSD
VTVGAPESMS KSKKNIIDPE ETVKIYGADS IRWFVLSDSP PDKDILWSDR GINAAYKFLQ
KVWNLNSQIN LLSERKGNLD IEKKYEKEFS KYILKIDDHI EKFNLNVMIA NIYEYFNALN
ECFKLSASKT FLEKHLINLM KIMFPITPHL SSECLETLKC KSDEWPSVDK KLVKDDEIKL
VVQINGKTRL VTSMKVDMEE NKITETLLKN DKLKKYLVEN KIKNKIFIKN KLINYIIK
//
