ID A0A0P9AG46_9CLOT Unreviewed; 426 AA.
AC A0A0P9AG46;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 27.
DE SubName: Full=Biotin synthase {ECO:0000313|EMBL:KPU44366.1};
DE EC=2.8.1.6 {ECO:0000313|EMBL:KPU44366.1};
GN Name=bioB_2 {ECO:0000313|EMBL:KPU44366.1};
GN ORFNames=OXPF_25360 {ECO:0000313|EMBL:KPU44366.1};
OS Oxobacter pfennigii.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae; Oxobacter.
OX NCBI_TaxID=36849 {ECO:0000313|EMBL:KPU44366.1, ECO:0000313|Proteomes:UP000050326};
RN [1] {ECO:0000313|EMBL:KPU44366.1, ECO:0000313|Proteomes:UP000050326}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 3222 {ECO:0000313|EMBL:KPU44366.1,
RC ECO:0000313|Proteomes:UP000050326};
RA Poehlein A., Bengelsdorf F.R., Schiel-Bengelsdorf B., Duerre P., Daniel R.;
RT "Genome sequence of Oxobacter pfennigii DSM 3222.";
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KPU44366.1}.
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DR EMBL; LKET01000032; KPU44366.1; -; Genomic_DNA.
DR RefSeq; WP_054875544.1; NZ_LKET01000032.1.
DR AlphaFoldDB; A0A0P9AG46; -.
DR STRING; 36849.OXPF_25360; -.
DR PATRIC; fig|36849.3.peg.2678; -.
DR OrthoDB; 9801424at2; -.
DR Proteomes; UP000050326; Unassembled WGS sequence.
DR GO; GO:0004076; F:biotin synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0031419; F:cobalamin binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd01335; Radical_SAM; 1.
DR CDD; cd02068; radical_SAM_B12_BD; 1.
DR Gene3D; 3.40.50.280; Cobalamin-binding domain; 1.
DR Gene3D; 3.80.30.20; tm_1862 like domain; 1.
DR InterPro; IPR006158; Cobalamin-bd.
DR InterPro; IPR025274; DUF4070.
DR InterPro; IPR006638; Elp3/MiaA/NifB-like_rSAM.
DR InterPro; IPR034466; Methyltransferase_Class_B.
DR InterPro; IPR007197; rSAM.
DR InterPro; IPR023404; rSAM_horseshoe.
DR PANTHER; PTHR43409; ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED; 1.
DR PANTHER; PTHR43409:SF12; BLL1977 PROTEIN; 1.
DR Pfam; PF02310; B12-binding; 1.
DR Pfam; PF13282; DUF4070; 1.
DR Pfam; PF04055; Radical_SAM; 1.
DR SFLD; SFLDG01082; B12-binding_domain_containing; 1.
DR SFLD; SFLDG01123; methyltransferase_(Class_B); 1.
DR SMART; SM00729; Elp3; 1.
DR SUPFAM; SSF102114; Radical SAM enzymes; 1.
DR PROSITE; PS51332; B12_BINDING; 1.
DR PROSITE; PS51918; RADICAL_SAM; 1.
PE 4: Predicted;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485};
KW Iron {ECO:0000256|ARBA:ARBA00022485};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00022485};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000050326};
KW Transferase {ECO:0000313|EMBL:KPU44366.1}.
FT DOMAIN 1..131
FT /note="B12-binding"
FT /evidence="ECO:0000259|PROSITE:PS51332"
FT DOMAIN 153..364
FT /note="Radical SAM core"
FT /evidence="ECO:0000259|PROSITE:PS51918"
SQ SEQUENCE 426 AA; 48761 MW; 9089E7C0616AEF5B CRC64;
MKLLLITASA HEIRQIRKTR VINFQQVTMP YLAALTPANW EVEHIDEGQE RIDFNIEVDL
VGITFHTPSA NHVYEIAAEF RKRGIPVAMG GPHVSLLPDE AENHADAVFI GEAEDTWKEF
LADFENKQFK KRYEQKNPSS LINLPMARKD LFHRRDHSGG IMFATRGCPN KCEFCALAIM
YSSNFRKRPV AEVAREYGSF EGKVIIFWDD NIAADPNYAK DLFKAIAPYK KWWSSQTSIH
AGWNDELLEL AAKSGCKQLF LGLESVSQIS LDHANKSFNR VEDYYKIIKK IHSYGISVQV
GIIFGFDEDD KSIFGKTMTF LEAAGVQNAT FNVLTPYPGT PLFKRLESEG RILTYDWSKY
NGRRDVVFKP KNMTCDELLE GFKRVNSQFY SLKSTGKRLS KSTAGLWWTL PLNLSYYFSY
KLYGPK
//
