ID   A0A0Q5Z986_9BURK        Unreviewed;       389 AA.
AC   A0A0Q5Z986;
DT   20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT   20-JAN-2016, sequence version 1.
DT   24-JAN-2024, entry version 31.
DE   SubName: Full=Mandelate racemase {ECO:0000313|EMBL:KQT11429.1};
GN   ORFNames=ASG30_06020 {ECO:0000313|EMBL:KQT11429.1};
OS   Ramlibacter sp. Leaf400.
OC   Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Ramlibacter.
OX   NCBI_TaxID=1736365 {ECO:0000313|EMBL:KQT11429.1, ECO:0000313|Proteomes:UP000051173};
RN   [1] {ECO:0000313|EMBL:KQT11429.1, ECO:0000313|Proteomes:UP000051173}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Leaf400 {ECO:0000313|EMBL:KQT11429.1,
RC   ECO:0000313|Proteomes:UP000051173};
RA   Gilbert D.G.;
RL   Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN   [2] {ECO:0000313|EMBL:KQT11429.1, ECO:0000313|Proteomes:UP000051173}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Leaf400 {ECO:0000313|EMBL:KQT11429.1,
RC   ECO:0000313|Proteomes:UP000051173};
RA   Schulze-Lefert P.;
RT   "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL   Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRSR:PIRSR634611-3};
CC       Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|PIRSR:PIRSR634611-3};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KQT11429.1}.
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DR   EMBL; LMQL01000009; KQT11429.1; -; Genomic_DNA.
DR   RefSeq; WP_055895451.1; NZ_LMQL01000009.1.
DR   AlphaFoldDB; A0A0Q5Z986; -.
DR   STRING; 1736365.ASG30_06020; -.
DR   OrthoDB; 9802699at2; -.
DR   Proteomes; UP000051173; Unassembled WGS sequence.
DR   GO; GO:0047808; F:D(-)-tartrate dehydratase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.120; Enolase-like C-terminal domain; 1.
DR   Gene3D; 3.30.390.10; Enolase-like, N-terminal domain; 1.
DR   InterPro; IPR034611; D-tartrate_dehydratase.
DR   InterPro; IPR036849; Enolase-like_C_sf.
DR   InterPro; IPR029017; Enolase-like_N.
DR   InterPro; IPR029065; Enolase_C-like.
DR   InterPro; IPR013342; Mandelate_racemase_C.
DR   InterPro; IPR046945; RHMD-like.
DR   PANTHER; PTHR13794; ENOLASE SUPERFAMILY, MANDELATE RACEMASE; 1.
DR   PANTHER; PTHR13794:SF58; MITOCHONDRIAL ENOLASE SUPERFAMILY MEMBER 1; 1.
DR   Pfam; PF13378; MR_MLE_C; 1.
DR   SFLD; SFLDF00118; D-tartrate_dehydratase; 1.
DR   SFLD; SFLDG00179; mandelate_racemase; 1.
DR   SMART; SM00922; MR_MLE; 1.
DR   SUPFAM; SSF51604; Enolase C-terminal domain-like; 1.
DR   SUPFAM; SSF54826; Enolase N-terminal domain-like; 1.
PE   4: Predicted;
KW   Magnesium {ECO:0000256|PIRSR:PIRSR634611-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR634611-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000051173}.
FT   DOMAIN          162..259
FT                   /note="Mandelate racemase/muconate lactonizing enzyme C-
FT                   terminal"
FT                   /evidence="ECO:0000259|SMART:SM00922"
FT   ACT_SITE        183
FT                   /note="acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT   ACT_SITE        321
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634611-1"
FT   BINDING         212
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT   BINDING         238
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
FT   BINDING         264
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR634611-3"
SQ   SEQUENCE   389 AA;  42085 MW;  C0CAE4214455C283 CRC64;
     MRITEVRELA VQLEGGVANA VVNFSEHTVS LVAVVTDVVR GGRPVVGLGF NSIGRFAQSG
     IIRDRMIPRL LSAPPASLLA EGGERFDAAQ VLATIMRNEK PGGHGDRAGA AAAIEHAIWD
     LNAKLADEPA YATIARAFGR EPVATPVEVY TGGGYYYAPG TGQTLRDEFL RYREMGFESF
     KMKIGGAPLA EDMKRVEQAL QVAGGGAHLM VDANGRFDLP TALAYAKELQ PLDLRWFEEI
     GDPLDYQLNR EVIEAYGGAV ATGENLFSAI DTLNLVRFGG MRPRRDIFQM DGGLSYGITE
     YARMIQVLES HGFDRRQAVP HGGHLINLHA VVGLGLGACE AYPGVFQPFG GYSKECEIRN
     AKVRPSDAPG FGLEQKAELA PFIRQLTGH
//