ID A0A0R2AXK8_9LACO Unreviewed; 916 AA.
AC A0A0R2AXK8;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE RecName: Full=HD Cas3-type domain-containing protein {ECO:0008006|Google:ProtNLM};
GN ORFNames=FC34_GL001110 {ECO:0000313|EMBL:KRM72126.1};
OS Lacticaseibacillus brantae DSM 23927.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Lacticaseibacillus.
OX NCBI_TaxID=1423727 {ECO:0000313|EMBL:KRM72126.1, ECO:0000313|Proteomes:UP000051672};
RN [1] {ECO:0000313|EMBL:KRM72126.1, ECO:0000313|Proteomes:UP000051672}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 23927 {ECO:0000313|EMBL:KRM72126.1,
RC ECO:0000313|Proteomes:UP000051672};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- SIMILARITY: In the N-terminal section; belongs to the CRISPR-associated
CC nuclease Cas3-HD family. {ECO:0000256|ARBA:ARBA00006847}.
CC -!- SIMILARITY: In the central section; belongs to the CRISPR-associated
CC helicase Cas3 family. {ECO:0000256|ARBA:ARBA00009046}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRM72126.1}.
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DR EMBL; AYZQ01000002; KRM72126.1; -; Genomic_DNA.
DR RefSeq; WP_057894394.1; NZ_AYZQ01000002.1.
DR AlphaFoldDB; A0A0R2AXK8; -.
DR STRING; 1423727.FC34_GL001110; -.
DR PATRIC; fig|1423727.3.peg.1124; -.
DR OrthoDB; 9810236at2; -.
DR Proteomes; UP000051672; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0051607; P:defense response to virus; IEA:UniProtKB-KW.
DR CDD; cd09641; Cas3''_I; 1.
DR Gene3D; 1.10.3210.30; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR041372; Cas3_C.
DR InterPro; IPR006483; CRISPR-assoc_Cas3_HD.
DR InterPro; IPR038257; CRISPR-assoc_Cas3_HD_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR006474; Helicase_Cas3_CRISPR-ass_core.
DR InterPro; IPR027417; P-loop_NTPase.
DR NCBIfam; TIGR01587; cas3_core; 1.
DR NCBIfam; TIGR01596; cas3_HD; 1.
DR PANTHER; PTHR47959; ATP-DEPENDENT RNA HELICASE RHLE-RELATED; 1.
DR PANTHER; PTHR47959:SF16; CRISPR-ASSOCIATED NUCLEASE_HELICASE CAS3-RELATED; 1.
DR Pfam; PF18395; Cas3_C; 1.
DR Pfam; PF18019; Cas3_HD; 1.
DR Pfam; PF00270; DEAD; 1.
DR SMART; SM00487; DEXDc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51643; HD_CAS3; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
PE 3: Inferred from homology;
KW Antiviral defense {ECO:0000256|ARBA:ARBA00023118};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000051672}.
FT DOMAIN 19..225
FT /note="HD Cas3-type"
FT /evidence="ECO:0000259|PROSITE:PS51643"
FT DOMAIN 302..506
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
SQ SEQUENCE 916 AA; 102462 MW; 127CE602410C938D CRC64;
MLNTREINAL WAKKNVQDGV YQWLPLITHL TDAKNVIEYL YNIWLSDGQR KAIAGDLSDE
AAHQLVRFLG ASHDIGKGSA AFQTQPSYRH DKGLDDEIME ELIRNGFQGI ADLNLASRRD
SPHALAGEAI LDYFGVPESI RAIIGGHHGK PGDSKSVKRD LNVHASNYFG NTRDPQSKAL
WESIQSSLFA WTLSEAGYAS VDEIPEVTQP QAVLLEGLLI MADWLASSEF LNDDPEKPLF
PLISLNETIE DIEAKTRFQS AMTNWTLEDN WNPTKVDLQA DPYKQRWGFD VRPVQAKMTQ
AIAKASDPGV VIVEAPMGIG KTEIALLAVE QLAYQTGRNG VFIGLPTQAT TNAMFSRAEA
WLAKIAAENH EQYNIRLMHG KAQFNQDYTQ LPRAQNVDDE QDGAVTVNAW FSGKKSILTE
FTVGTIDNLL LMGLKQKHLF LRHLGFSGKV VVIDEAHGFD SYMNKYLYKA LEWLGAYHVP
VIILSATLPV AKRQQMLTSY LLGKYGARAK IQSQVPDWET TQAYPLLSLL DGMELVQETD
FGQLTNGSEV QIEYLQADDE ALVQHIVAEI ADGGIAGVIV NTVKRAQELA ALIPDDVNRL
VLHSAFLAPD RSARESELLD LIGKHGKRPD QMIIIGTQVL EQSLDIDFDV LYTDIAPMDL
ILQRIGRLHR HKIDRPQQLQ TPKTFILGVA DDGYGDGNEA IYSKYLLAKT DYFLPKRIRL
PADISPLVQA VYTEETDTEI VGIDRLRADF DALQEEKESR AQVFQIHQPK LKLGSSIHRW
LQRLPMDVNT NDQKAAAAVR DIQESIEVVL LKQVNGEKFL LDGRAISQVS AQEIAQQTIR
LPNAVTPNID RAIDELERAT RKQYSQWETN VWLKGALVLT LDAQQQATLG QWQLQYSGVT
GLQYAKEDTI DETNEL
//
