ID A0A0R2JWK9_9LACO Unreviewed; 573 AA.
AC A0A0R2JWK9;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 29.
DE RecName: Full=Rqc2 homolog RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
DE Short=RqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN Name=rqcH {ECO:0000256|HAMAP-Rule:MF_00844};
GN ORFNames=IV52_GL000407 {ECO:0000313|EMBL:KRN79002.1};
OS Fructilactobacillus lindneri DSM 20690 = JCM 11027.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Fructilactobacillus.
OX NCBI_TaxID=1122148 {ECO:0000313|EMBL:KRN79002.1, ECO:0000313|Proteomes:UP000051565};
RN [1] {ECO:0000313|EMBL:KRN79002.1, ECO:0000313|Proteomes:UP000051565}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 20690 {ECO:0000313|EMBL:KRN79002.1,
RC ECO:0000313|Proteomes:UP000051565};
RX PubMed=26415554; DOI=10.1038/ncomms9322;
RA Sun Z., Harris H.M., McCann A., Guo C., Argimon S., Zhang W., Yang X.,
RA Jeffery I.B., Cooney J.C., Kagawa T.F., Liu W., Song Y., Salvetti E.,
RA Wrobel A., Rasinkangas P., Parkhill J., Rea M.C., O'Sullivan O., Ritari J.,
RA Douillard F.P., Paul Ross R., Yang R., Briner A.E., Felis G.E.,
RA de Vos W.M., Barrangou R., Klaenhammer T.R., Caufield P.W., Cui Y.,
RA Zhang H., O'Toole P.W.;
RT "Expanding the biotechnology potential of lactobacilli through comparative
RT genomics of 213 strains and associated genera.";
RL Nat. Commun. 6:8322-8322(2015).
CC -!- FUNCTION: Part of the ribosome quality control system (RQC). Recruits
CC Ala-charged tRNA and directs the elongation of stalled nascent chains
CC on 50S ribosomal subunits, leading to non-templated C-terminal Ala
CC extensions (Ala tail). The Ala tail promotes nascent chain degradation.
CC May add between 1 and at least 8 Ala residues. Binds to stalled 50S
CC ribosomal subunits. {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SUBUNIT: Associates with stalled 50S ribosomal subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00844}.
CC -!- SIMILARITY: Belongs to the NEMF family. {ECO:0000256|HAMAP-
CC Rule:MF_00844}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRN79002.1}.
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DR EMBL; JQBT01000032; KRN79002.1; -; Genomic_DNA.
DR RefSeq; WP_056997607.1; NZ_MIYK01000001.1.
DR AlphaFoldDB; A0A0R2JWK9; -.
DR STRING; 53444.AYR59_05475; -.
DR GeneID; 61250286; -.
DR PATRIC; fig|1122148.6.peg.426; -.
DR OrthoDB; 9766163at2; -.
DR Proteomes; UP000051565; Unassembled WGS sequence.
DR GO; GO:0043023; F:ribosomal large subunit binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0072344; P:rescue of stalled ribosome; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.970.40; fibrinogen binding protein from staphylococcus aureus domain like; 1.
DR Gene3D; 2.30.310.10; ibrinogen binding protein from staphylococcus aureus domain; 1.
DR HAMAP; MF_00844_B; RqcH_B; 1.
DR InterPro; IPR008532; NFACT_RNA-bd.
DR InterPro; IPR043682; RqcH_bacterial.
DR PANTHER; PTHR15239; NUCLEAR EXPORT MEDIATOR FACTOR NEMF; 1.
DR PANTHER; PTHR15239:SF6; RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF; 1.
DR Pfam; PF05670; NFACT-R_1; 1.
DR Pfam; PF18297; NFACT-R_2; 1.
DR Pfam; PF05833; NFACT_N; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|HAMAP-Rule:MF_00844};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00844}; Reference proteome {ECO:0000313|Proteomes:UP000051565};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|HAMAP-
KW Rule:MF_00844};
KW tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW Rule:MF_00844}.
FT DOMAIN 450..536
FT /note="NFACT RNA-binding"
FT /evidence="ECO:0000259|Pfam:PF05670"
FT COILED 295..322
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00844"
SQ SEQUENCE 573 AA; 66168 MW; 5D6A97AC15C423F1 CRC64;
MSFDGSFMHA MSNELNNELL NGRVTKINEP YPNEIMMTIR ANGNNYQTLL SANPLYARIQ
TTKIPFTNPK EPSNFAMTLR KRLRGSFLNK IHQVDNDRVM HFHFNTRNEL GDIEELVLVI
EVMARHSNII LVEAKNRNII DAVKRIGLEK NRYRTLLPGS KYINPPKQNM INPFDLNDFT
TINQLNHDFP NVSVFATNLR QYLQGLGKDT STALATAIHE PGSTIKSKYQ NFFSNFNNPT
PTISISENGK LNFSAFPYLN QKIEKQFPTL SEMLDVYFQT KVNHERVREQ GSQLIQITQN
ELKKNKRKIK RLEQSEKESK LADEYRIKGE ILMTYLKQVK SGEKLIVLPN FYDNNKKIKI
NLKPSLSPSA NAQWYFKQYQ KKKNAVKFIN QQLKKTKAEI DYFENIKSQI EIADPENLAD
IKIELQNEGY LKIKNKVKKQ RKAKVSKPER FVSDNGTIIL VGKNNLQNDQ LTHKIADKRD
TWLHTQKIHG SHVIIRSFNP SEETIIQAAE LAAYYSKARN SANVPVDYDV VKRIKKPSGA
KPGFVTYTGQ KTVYVTPTKE KFKHLRENVL KNN
//
