ID A0A0R4IAT4_DANRE Unreviewed; 459 AA.
AC A0A0R4IAT4; A0A8M2BGC6;
DT 20-JAN-2016, integrated into UniProtKB/TrEMBL.
DT 20-JAN-2016, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE SubName: Full=Methyltransferase NSUN6 isoform X1 {ECO:0000313|RefSeq:XP_005169112.1, ECO:0000313|RefSeq:XP_017212638.1};
DE SubName: Full=NOP2/Sun RNA methyltransferase 6 {ECO:0000313|Ensembl:ENSDARP00000131024};
GN Name=nsun6 {ECO:0000313|Ensembl:ENSDARP00000131024,
GN ECO:0000313|RefSeq:XP_005169112.1, ECO:0000313|RefSeq:XP_017212638.1,
GN ECO:0000313|RefSeq:XP_017212639.1, ECO:0000313|RefSeq:XP_017212640.1,
GN ECO:0000313|ZFIN:ZDB-GENE-080808-8};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000313|Ensembl:ENSDARP00000131024};
RN [1] {ECO:0000313|Ensembl:ENSDARP00000131024, ECO:0000313|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000131024};
RX PubMed=23594743; DOI=10.1038/nature12111;
RG Genome Reference Consortium Zebrafish;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Eliott D.,
RA Threadgold G., Harden G., Ware D., Begum S., Mortimore B., Mortimer B.,
RA Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M.,
RA Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M.,
RA Glithero R., Howden P., Barker N., Lloyd C., Stevens C., Harley J.,
RA Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D.,
RA Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K.,
RA Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R.,
RA Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M.,
RA Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D.,
RA Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M.,
RA Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M.,
RA Woodmansey R., Clark G., Cooper J., Cooper J., Tromans A., Grafham D.,
RA Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J.,
RA Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I.,
RA Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M.,
RA Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M.,
RA Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G., Osoegawa K., Zhu B.,
RA Rapp A., Widaa S., Langford C., Yang F., Schuster S.C., Carter N.P.,
RA Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R.,
RA Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I.,
RA Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H.,
RA Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000313|Ensembl:ENSDARP00000131024}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|Ensembl:ENSDARP00000131024};
RG Ensembl;
RL Submitted (NOV-2015) to UniProtKB.
RN [3] {ECO:0000313|RefSeq:XP_005169112.1, ECO:0000313|RefSeq:XP_017212638.1}
RP IDENTIFICATION.
RC STRAIN=Tuebingen {ECO:0000313|RefSeq:XP_005169112.1,
RC ECO:0000313|RefSeq:XP_017212638.1};
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. RsmB/NOP family. {ECO:0000256|ARBA:ARBA00007494,
CC ECO:0000256|PROSITE-ProRule:PRU01023}.
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DR EMBL; CU607104; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_005169112.1; XM_005169055.4.
DR RefSeq; XP_009301920.1; XM_009303645.2.
DR RefSeq; XP_017212637.1; XM_017357148.1.
DR RefSeq; XP_017212638.1; XM_017357149.2.
DR RefSeq; XP_017212639.1; XM_017357150.2.
DR RefSeq; XP_017212640.1; XM_017357151.2.
DR STRING; 7955.ENSDARP00000131024; -.
DR PaxDb; 7955-ENSDARP00000088711; -.
DR Ensembl; ENSDART00000158782.2; ENSDARP00000131024.1; ENSDARG00000103478.3.
DR Ensembl; ENSDART00000161344.3; ENSDARP00000137583.1; ENSDARG00000103478.3.
DR Ensembl; ENSDART00000189745.1; ENSDARP00000153997.1; ENSDARG00000103478.3.
DR GeneID; 100149557; -.
DR KEGG; dre:100149557; -.
DR AGR; ZFIN:ZDB-GENE-080808-8; -.
DR CTD; 221078; -.
DR ZFIN; ZDB-GENE-080808-8; nsun6.
DR eggNOG; KOG1122; Eukaryota.
DR OMA; YQGAMLY; -.
DR OrthoDB; 1077100at2759; -.
DR Proteomes; UP000000437; Chromosome 7.
DR Bgee; ENSDARG00000103478; Expressed in early embryo and 16 other cell types or tissues.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0008173; F:RNA methyltransferase activity; IEA:InterPro.
DR GO; GO:0001510; P:RNA methylation; IBA:GO_Central.
DR CDD; cd02440; AdoMet_MTases; 1.
DR CDD; cd21150; PUA_NSun6-like; 1.
DR Gene3D; 2.30.130.10; PUA domain; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR018314; Fmu/NOL1/Nop2p_CS.
DR InterPro; IPR049560; MeTrfase_RsmB-F_NOP2_cat.
DR InterPro; IPR001678; MeTrfase_RsmB-F_NOP2_dom.
DR InterPro; IPR015947; PUA-like_sf.
DR InterPro; IPR036974; PUA_sf.
DR InterPro; IPR023267; RCMT.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR22807; NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR22807:SF34; TRNA (CYTOSINE(72)-C(5))-METHYLTRANSFERASE NSUN6; 1.
DR Pfam; PF01189; Methyltr_RsmB-F; 1.
DR PRINTS; PR02008; RCMTFAMILY.
DR SUPFAM; SSF88697; PUA domain-like; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01153; NOL1_NOP2_SUN; 1.
DR PROSITE; PS50890; PUA; 1.
DR PROSITE; PS51686; SAM_MT_RSMB_NOP; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01023}; Reference proteome {ECO:0000313|Proteomes:UP000000437};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01023};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01023};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01023}.
FT DOMAIN 139..459
FT /note="SAM-dependent MTase RsmB/NOP-type"
FT /evidence="ECO:0000259|PROSITE:PS51686"
FT ACT_SITE 371
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 240..246
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 264
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 299
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
FT BINDING 321
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01023"
SQ SEQUENCE 459 AA; 50520 MW; 22C12F9AD0709B51 CRC64;
MAVFPQLSMS PEVREHLHTV YTNDEAVCEL GAGVAEQQFE ALLWCLSHPP LYTCLRVSTH
LHPLRDVQHR LQQHLEQIQC RCPAVHTHPE LPDVLLLPVC GPRPVEPVAS AVIVGSQCGS
AVLRGAHVFT PGIISTHKFM KAGDVVSVFS DVEGKCTRGA TEFNGKKVFV GNGVSEVNRS
ELFSSDKPGR GLAIRMTEPL YQSPSFDGVL TDELFLQNLP SVVVGYVLGP RPGERVLDMC
AAPGGKTTHI ASLMGNQGVV VALEKVRSKM EKILRNAQML KLDCIKAYCC NSIHAVSSDP
AQEYTEGPPF PEESFDRILL DAPCSGLGQR PNMSYSWSLK EVCSYQPLQR KLFTTAVRLL
KSGGVLVYST CTVTLAENEE QVSWALKTFP CLTLQPQVPH VGSEGMLGAG LSCEQRRLLQ
RFRPELGWTG ERFTFSLEKL PQDANQDTIG FFIAKFSKR
//
