ID MPAO_CLAUC Reviewed; 445 AA.
AC A0A0R8YXT5;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 16.
DE RecName: Full=Methylphloroacetophenone oxidase {ECO:0000303|PubMed:26895859};
DE Short=MPAO {ECO:0000303|PubMed:26895859};
DE EC=1.-.-.- {ECO:0000305};
DE AltName: Full=Cytochrome P450 monooxygenase MPAO {ECO:0000303|PubMed:26895859};
DE AltName: Full=Usnic acid biosynthesis protein MPAO {ECO:0000305};
GN Name=MPAO {ECO:0000303|PubMed:26895859};
OS Cladonia uncialis (Cup lichen).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Lecanoromycetes;
OC OSLEUM clade; Lecanoromycetidae; Lecanorales; Lecanorineae; Cladoniaceae;
OC Cladonia.
OX NCBI_TaxID=174080;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND INDUCTION.
RX PubMed=26895859; DOI=10.1016/j.funbio.2015.10.009;
RA Abdel-Hameed M.D., Bertrand R.L., Piercey-Normore M.D., Sorensen J.L.;
RT "Putative identification of the usnic acid biosynthetic gene cluster by de
RT novo whole-genome sequencing of a lichen-forming fungus.";
RL Fungal Biol. 120:306-316(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Wen L., He K., Yang H.;
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP REVIEW ON BIOTECHNOLOGY.
RX PubMed=25707417; DOI=10.1080/14786419.2015.1007455;
RA Araujo A.A., de Melo M.G., Rabelo T.K., Nunes P.S., Santos S.L.,
RA Serafini M.R., Santos M.R., Quintans-Junior L.J., Gelain D.P.;
RT "Review of the biological properties and toxicity of usnic acid.";
RL Nat. Prod. Res. 29:2167-2180(2015).
CC -!- FUNCTION: Methylphloroacetophenone oxidase; part of the gene cluster
CC that mediates the biosynthesis of usnic acid, a dibenzofuran lichen
CC product possessing a broad spectrum of biological activities
CC (PubMed:26895859). Two genes, mpas and mpao, comprise the usnic acid
CC biosynthetic gene cluster with a single post-PKS enzyme, the
CC methylphloracetophenone oxidase (mpao) (PubMed:26895859). The
CC methylphloroacetophenone synthase (mpas) is a non-reducing polyketide
CC synthase that produces methylphloracetophenone from acetate via a
CC methylated tetraketide intermediate (PubMed:26895859). The
CC methylphloroacetophenone oxidase then carries out the oxidative
CC dimerization of methylphloracetophenone to usnic acid
CC (PubMed:26895859). {ECO:0000269|PubMed:26895859}.
CC -!- PATHWAY: Secondary metabolite biosynthesis.
CC {ECO:0000269|PubMed:26895859}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass membrane
CC protein {ECO:0000255}.
CC -!- BIOTECHNOLOGY: Lichens belonging to usnic acid-containing genera have
CC been used as crude drugs throughout the world (PubMed:25707417). Usnic
CC acid is a potentially interesting candidate for such activities as
CC anti-inflammatory, analgesic, healing, antioxidant, antimicrobial,
CC antiprotozoal, antiviral, larvicidal and UV protection
CC (PubMed:25707417). However, it was shown to be toxic for the liver and
CC to lead to contact allergy (PubMed:25707417).
CC {ECO:0000269|PubMed:25707417}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; KT363733; ALA62324.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0R8YXT5; -.
DR GlyCosmos; A0A0R8YXT5; 1 site, No reported glycans.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; Cytochrome P450; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR036396; Cyt_P450_sf.
DR PANTHER; PTHR24305; CYTOCHROME P450; 1.
DR PANTHER; PTHR24305:SF96; CYTOCHROME P450 MONOOXYGENASE STCB-RELATED; 1.
DR Pfam; PF00067; p450; 1.
DR SUPFAM; SSF48264; Cytochrome P450; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Oxidoreductase; Transmembrane; Transmembrane helix.
FT CHAIN 1..445
FT /note="Methylphloroacetophenone oxidase"
FT /id="PRO_0000437592"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 51
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ SEQUENCE 445 AA; 49443 MW; 7A8E55718FDC0A77 CRC64;
MISPVSSILA SIWDNSKLLL DHTSVLSIAL IGVACAISIR SILYVRLACA NYSILLLTHA
QRLRLAYSTP LRHVPGPWYA KFTALGLRAN DVAGNRWYYV QGLHKKYGSI VRIAPEEVAI
SDPKVVSKVH ALGTEFRKRQ QPGTPFNIFS ISDPKAHRTR QRFYAKAFSD ETLKASTEPA
VRQLIKTAVA SIKRDAALRK DHTADVYKWC MLFGSDVAFQ VIYGNSNTEG LMATQKTTDE
VIMGAYLQRM NAWAQFCFPV FLLGRWLSPL SPTLHNIFRV EEKYGDFWQE GQRQREIAAR
TVFVQNTKYS KNDGVFSVSD EVKLSDVDIA HDITTFLGAG GEPVGASLVF LIWQVLRMPD
LQRELEAEVA GLTEPITDAT TAQLPILNGV IYETLRLYGG GVTQMPRYAP IATELGGYVI
PPGTAVTTHT GALHRNPAAW DDPEK
//
