ID A0A0S9R5C5_9ACTN Unreviewed; 1161 AA.
AC A0A0S9R5C5;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE RecName: Full=Transcription-repair-coupling factor {ECO:0000256|HAMAP-Rule:MF_00969};
DE Short=TRCF {ECO:0000256|HAMAP-Rule:MF_00969};
DE EC=3.6.4.- {ECO:0000256|HAMAP-Rule:MF_00969};
GN Name=mfd {ECO:0000256|HAMAP-Rule:MF_00969};
GN ORFNames=ASF37_12050 {ECO:0000313|EMBL:KQP77281.1};
OS Aeromicrobium sp. Leaf289.
OC Bacteria; Actinomycetota; Actinomycetes; Propionibacteriales;
OC Nocardioidaceae; Aeromicrobium.
OX NCBI_TaxID=1736324 {ECO:0000313|EMBL:KQP77281.1, ECO:0000313|Proteomes:UP000051640};
RN [1] {ECO:0000313|Proteomes:UP000051640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf289 {ECO:0000313|Proteomes:UP000051640};
RA Garrido-Oter R., Mueller D.B.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000051640}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Leaf289 {ECO:0000313|Proteomes:UP000051640};
RA Vorholt J.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Couples transcription and DNA repair by recognizing RNA
CC polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent
CC release of RNAP and its truncated transcript from the DNA, and
CC recruitment of nucleotide excision repair machinery to the damaged
CC site. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the helicase family.
CC RecG subfamily. {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the UvrB family.
CC {ECO:0000256|HAMAP-Rule:MF_00969}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQP77281.1}.
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DR EMBL; LMNK01000003; KQP77281.1; -; Genomic_DNA.
DR RefSeq; WP_056552430.1; NZ_LMNK01000003.1.
DR AlphaFoldDB; A0A0S9R5C5; -.
DR OrthoDB; 9804325at2; -.
DR Proteomes; UP000051640; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003684; F:damaged DNA binding; IEA:InterPro.
DR GO; GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of DNA-templated transcription; IEA:UniProtKB-UniRule.
DR GO; GO:0000716; P:transcription-coupled nucleotide-excision repair, DNA damage recognition; IEA:UniProtKB-UniRule.
DR CDD; cd17991; DEXHc_TRCF; 1.
DR Gene3D; 2.40.10.170; -; 1.
DR Gene3D; 3.40.50.11180; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR Gene3D; 3.30.2060.10; Penicillin-binding protein 1b domain; 1.
DR Gene3D; 3.90.1150.50; Transcription-repair-coupling factor, D7 domain; 1.
DR HAMAP; MF_00969; TRCF; 1.
DR InterPro; IPR003711; CarD-like/TRCF_RID.
DR InterPro; IPR036101; CarD-like/TRCF_RID_sf.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR004576; Mfd.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR047112; RecG/Mfd.
DR InterPro; IPR037235; TRCF-like_C_D7.
DR InterPro; IPR005118; TRCF_C.
DR InterPro; IPR041471; UvrB_inter.
DR NCBIfam; TIGR00580; mfd; 1.
DR PANTHER; PTHR47964; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR PANTHER; PTHR47964:SF1; ATP-DEPENDENT DNA HELICASE HOMOLOG RECG, CHLOROPLASTIC; 1.
DR Pfam; PF02559; CarD_TRCF_RID; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF03461; TRCF; 1.
DR Pfam; PF17757; UvrB_inter; 1.
DR SMART; SM01058; CarD_TRCF; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00982; TRCF; 1.
DR SUPFAM; SSF141259; CarD-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
DR SUPFAM; SSF143517; TRCF domain-like; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00969};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204, ECO:0000256|HAMAP-
KW Rule:MF_00969};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Helicase {ECO:0000256|ARBA:ARBA00022806};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00969};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00969}; Reference proteome {ECO:0000313|Proteomes:UP000051640}.
FT DOMAIN 629..790
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 808..965
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
SQ SEQUENCE 1161 AA; 126305 MW; 23197EDEA3686897 CRC64;
MDLHALLSPH LAGSLADVVS QRPRTVTLQG PAALRPFVAR AAADASTVLA VTPTAREADE
LAADLSVLLG DDAVVVYPAW ETLPHERLSP RSDTVGRRLA VLRRLAHPGA GAPPVRVVVA
PVRSILQPQV AGLADLTPVE LVVGQQAPLD DVVRDLAAAA YVRVDLVERR GEFAVRGGIV
DVFPPTEDHP LRIDFWGDEV EEIRWFSVAD QRTTDAVERL WAPPCRELLL TDEVRGRAAA
LATEHPALAE LFGKVAEGHA VEGMESLAPV LVDELELLVE VLPSDAVVVV NDPEKVRARA
ADLNATNEEF LQASWAAAAT GAAAPIDLGA AAFKELEEVR AVALARGLAW WNLSPFGLDD
EDTVVVPAEA APLYRGDTEQ AVADVRGWLA DGRFVVVAAP AAGQAQRTVE WLAEHDVPAA
LVDGDTPAPE AGSGVVAVLV ADVDHGLVAA KAVLLTHDDL VAQRAAERPD RSSMPVRRRK
QVDPLELKAG DHVVHEQHGV GRYVELVNRT IQGAAREYLV IEYAPSKRGQ PGDRLFVPMD
QLDLVSRYVG GESPSLDKLG GADWTARKNK ARKAVRQIAG ELIKLYAARQ STKGHAFGPD
TPWQAELEDA FAFVETPDQL ITIDEVKRDM ERQVPMDRLV CGDVGYGKTE IAVRAAFKAI
QDGKQVILLV PTTLLVQQHY ATFAERFGQF PVTIKPLSRF QTEKESQATL DGLADGSVDL
VVGTHRLLQP GVRIKDLGLV IVDEEQRFGV EHKEALKHLR AAVDVLAMSA TPIPRTLEMA
VTGIREMSTI ATPPEERHPV LSFVGAYDDK QVAAAIRREL LREGQAFFIH NRVQSIDKVV
RRLGELVPEA RIAAAHGQMG EHQLEKVMVD FWEKRYDVLV CTTIVESGLD VSNANTMIIE
RSDTLGLSQL HQLRGRVGRG RERAYAYFLY PPDKPLTETA HDRLATIAQH SELGGGMQVA
MKDLEIRGAG NLLGGEQSGH IADVGFDLYV RLVGEAVAEF RGEGEAGPQG AREVKLELPV
EAHLPHDYVP SERLRLEMYK RLADVRSLDD VAELRAELVD RYGEPPEVAE VLLDVARLRV
AVRAAGLTEV TSAGSQIRFG GLSLPESAQM RLRRVYPKSI YKPATDVALV PAPRTAPVGG
RPLRGRELLE WVDTVVQTLV A
//
