ID A0A0T1WDI0_9MYCO Unreviewed; 233 AA.
AC A0A0T1WDI0;
DT 17-FEB-2016, integrated into UniProtKB/TrEMBL.
DT 17-FEB-2016, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE SubName: Full=Thiocyanate hydrolase subunit gamma {ECO:0000313|EMBL:KQY07195.1};
GN ORFNames=ASD37_14085 {ECO:0000313|EMBL:KQY07195.1};
OS Mycobacterium sp. Root135.
OC Bacteria; Actinomycetota; Actinomycetes; Mycobacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=1736457 {ECO:0000313|EMBL:KQY07195.1, ECO:0000313|Proteomes:UP000051127};
RN [1] {ECO:0000313|EMBL:KQY07195.1, ECO:0000313|Proteomes:UP000051127}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root135 {ECO:0000313|EMBL:KQY07195.1,
RC ECO:0000313|Proteomes:UP000051127};
RA Gilbert D.G.;
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:KQY07195.1, ECO:0000313|Proteomes:UP000051127}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Root135 {ECO:0000313|EMBL:KQY07195.1,
RC ECO:0000313|Proteomes:UP000051127};
RA Schulze-Lefert P.;
RT "Functional overlap of the Arabidopsis leaf and root microbiotas.";
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the nitrile hydratase subunit alpha family.
CC {ECO:0000256|ARBA:ARBA00009363}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KQY07195.1}.
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DR EMBL; LMEZ01000005; KQY07195.1; -; Genomic_DNA.
DR RefSeq; WP_056551944.1; NZ_LMEZ01000005.1.
DR AlphaFoldDB; A0A0T1WDI0; -.
DR STRING; 1736457.ASD37_14085; -.
DR OrthoDB; 528553at2; -.
DR Proteomes; UP000051127; Unassembled WGS sequence.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0046914; F:transition metal ion binding; IEA:InterPro.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR Gene3D; 3.90.330.10; Nitrile hydratase alpha /Thiocyanate hydrolase gamma; 1.
DR InterPro; IPR036648; CN_Hdrase_a/SCN_Hdrase_g_sf.
DR InterPro; IPR004232; CN_Hdrtase_a/SCN_Hdrlase_g.
DR InterPro; IPR023900; CN_Hdrtase_asu/SCN_Hdrlase_gsu.
DR InterPro; IPR023901; Thiocyan_Hydrolase_gsu.
DR NCBIfam; TIGR03887; thiocyan_alph; 1.
DR Pfam; PF02979; NHase_alpha; 1.
DR PIRSF; PIRSF001426; NHase_alpha; 1.
DR SUPFAM; SSF56209; Nitrile hydratase alpha chain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:KQY07195.1};
KW Iron {ECO:0000256|PIRSR:PIRSR001426-1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR001426-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000051127}.
FT DOMAIN 20..210
FT /note="Nitrile hydratase alpha /Thiocyanate hydrolase
FT gamma"
FT /evidence="ECO:0000259|Pfam:PF02979"
FT BINDING 120
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
FT BINDING 123
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
FT BINDING 124
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
FT BINDING 125
FT /ligand="Fe(3+)"
FT /ligand_id="ChEBI:CHEBI:29034"
FT /evidence="ECO:0000256|PIRSR:PIRSR001426-1"
SQ SEQUENCE 233 AA; 26337 MW; ABF850A181264841 CRC64;
MTHDHDHDRT VKPMVDEITD FEVLEIALRE LCIEKGIFTA AEHRLFTEFA EQIGPTPAAR
LVARAWLDPD FERLALTEPM TASKEVGVDW LEPTGFGTPS DFTAFQILAD TPTLHHVIVC
ALCSCYPRPI LGNSPEWYRT PNYRRRLVRW PRQVLAEFGL YLGDDVEVRV QDSNQKHRFM
VMPVRPAGTD DWTEDQLVEI ITRDCLIGVA LPKAGVTTNV VTATRAAMHP VGE
//
