ID A0A0V1I0B2_9BILA Unreviewed; 427 AA.
AC A0A0V1I0B2;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 21.
DE RecName: Full=tRNA pseudouridine synthase {ECO:0000256|RuleBase:RU003792};
DE EC=5.4.99.12 {ECO:0000256|RuleBase:RU003792};
GN Name=Pus3 {ECO:0000313|EMBL:KRZ16143.1};
GN ORFNames=T11_1349 {ECO:0000313|EMBL:KRZ16143.1};
OS Trichinella zimbabwensis.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Enoplea; Dorylaimia;
OC Trichinellida; Trichinellidae; Trichinella.
OX NCBI_TaxID=268475 {ECO:0000313|EMBL:KRZ16143.1, ECO:0000313|Proteomes:UP000055024};
RN [1] {ECO:0000313|EMBL:KRZ16143.1, ECO:0000313|Proteomes:UP000055024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ISS1029 {ECO:0000313|EMBL:KRZ16143.1};
RA Korhonen P.K., Edoardo P., Giuseppe L.R., Gasser R.B.;
RT "Evolution of Trichinella species and genotypes.";
RL Submitted (JAN-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA;
CC Xref=Rhea:RHEA:22376, Rhea:RHEA-COMP:10085, Rhea:RHEA-COMP:10087,
CC ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.12;
CC Evidence={ECO:0000256|RuleBase:RU003792};
CC -!- SIMILARITY: Belongs to the tRNA pseudouridine synthase TruA family.
CC {ECO:0000256|ARBA:ARBA00009375, ECO:0000256|RuleBase:RU003792}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KRZ16143.1}.
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DR EMBL; JYDP01000013; KRZ16143.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0V1I0B2; -.
DR STRING; 268475.A0A0V1I0B2; -.
DR Proteomes; UP000055024; Unassembled WGS sequence.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0106029; F:tRNA pseudouridine synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0001522; P:pseudouridine synthesis; IEA:InterPro.
DR GO; GO:0008033; P:tRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 3.30.70.660; Pseudouridine synthase I, catalytic domain, C-terminal subdomain; 1.
DR Gene3D; 3.30.70.580; Pseudouridine synthase I, catalytic domain, N-terminal subdomain; 1.
DR HAMAP; MF_00171; TruA; 1.
DR InterPro; IPR020103; PsdUridine_synth_cat_dom_sf.
DR InterPro; IPR001406; PsdUridine_synth_TruA.
DR InterPro; IPR020097; PsdUridine_synth_TruA_a/b_dom.
DR InterPro; IPR020095; PsdUridine_synth_TruA_C.
DR InterPro; IPR020094; TruA/RsuA/RluB/E/F_N.
DR NCBIfam; TIGR00071; hisT_truA; 1.
DR PANTHER; PTHR11142; PSEUDOURIDYLATE SYNTHASE; 1.
DR PANTHER; PTHR11142:SF5; TRNA PSEUDOURIDINE(38_39) SYNTHASE; 1.
DR Pfam; PF01416; PseudoU_synth_1; 1.
DR SUPFAM; SSF55120; Pseudouridine synthase; 1.
PE 3: Inferred from homology;
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000256|RuleBase:RU003792};
KW Reference proteome {ECO:0000313|Proteomes:UP000055024};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694,
KW ECO:0000256|RuleBase:RU003792}.
FT DOMAIN 193..307
FT /note="Pseudouridine synthase I TruA alpha/beta"
FT /evidence="ECO:0000259|Pfam:PF01416"
FT REGION 393..427
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 427 AA; 49001 MW; 3D89F310BC22AE6B CRC64;
MFIFLLISFD NFVSEMPRTV ERQFDFNRYC RRHIALMVAY LGWDLDGFCA QNNSQNTVES
KIFKALIETK LIQSLETANY SRCGRTDKTV SAFSQVKLVL SLDVRCKQTT GVGVFEPDGY
CPNEPEPTSR KTTPKSEELD YAKILNSVLP QEIRVLAWAP VERTFNARYN CTSRQYKYFF
PKGALNLKLM EQASKWLIGK HDFRNFCKID MSKNEIQSCV RTVFDVQFTK VNASGLNSRT
LCCFTIVANA FLWHQIRCIM SVLFAIGEGK EQPDVIEWLL NTELCPAKPQ YSMVVGWPLV
LYGCQYDNLE WHWKEGIIME CISSLQRTWV NFAIKVEIIE NMMNSLLSLI PNNSATADLN
TTSILHPELL SHSYRPLMGR SVGKSLEEKI AKKKKQQLET KQTTADDTTK QSLKEEEEEE
AVENLSG
//
