ID A0A0W1DLW8_9SPHN Unreviewed; 537 AA.
AC A0A0W1DLW8;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 27-MAR-2024, entry version 33.
DE RecName: Full=Alkaline phosphatase {ECO:0000256|PIRNR:PIRNR031924};
DE EC=3.1.3.1 {ECO:0000256|PIRNR:PIRNR031924};
GN ORFNames=ATE67_15890 {ECO:0000313|EMBL:KTE19147.1};
OS Sphingopyxis sp. H050.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingopyxis.
OX NCBI_TaxID=1759072 {ECO:0000313|EMBL:KTE19147.1, ECO:0000313|Proteomes:UP000053439};
RN [1] {ECO:0000313|Proteomes:UP000053439}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H050 {ECO:0000313|Proteomes:UP000053439};
RA Gomez-Alvarez V., Revetta R.P.;
RL Submitted (NOV-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Alkaline phosphatase with broad substrate specificity.
CC {ECO:0000256|PIRNR:PIRNR031924}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.1;
CC Evidence={ECO:0000256|PIRNR:PIRNR031924};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|PIRNR:PIRNR031924};
CC Note=Binds 2 Zn(2+) ions. {ECO:0000256|PIRNR:PIRNR031924};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KTE19147.1}.
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DR EMBL; LNRZ01000008; KTE19147.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0W1DLW8; -.
DR STRING; 1759072.ATE67_15890; -.
DR Proteomes; UP000053439; Unassembled WGS sequence.
DR GO; GO:0004035; F:alkaline phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR CDD; cd16016; AP-SPAP; 1.
DR Gene3D; 3.30.1360.150; -; 1.
DR Gene3D; 3.40.720.10; Alkaline Phosphatase, subunit A; 1.
DR InterPro; IPR017850; Alkaline_phosphatase_core_sf.
DR InterPro; IPR026263; Alkaline_phosphatase_prok.
DR InterPro; IPR002591; Phosphodiest/P_Trfase.
DR PANTHER; PTHR10151:SF120; BIS(5'-ADENOSYL)-TRIPHOSPHATASE; 1.
DR PANTHER; PTHR10151; ECTONUCLEOTIDE PYROPHOSPHATASE/PHOSPHODIESTERASE; 1.
DR Pfam; PF01663; Phosphodiest; 1.
DR PIRSF; PIRSF031924; Pi-irrepressible_AP; 1.
DR SUPFAM; SSF53649; Alkaline phosphatase-like; 1.
PE 4: Predicted;
KW Metal-binding {ECO:0000256|PIRNR:PIRNR031924};
KW Phosphoprotein {ECO:0000256|PIRSR:PIRSR031924-50};
KW Zinc {ECO:0000256|PIRNR:PIRNR031924}.
FT ACT_SITE 68
FT /note="Phosphothreonine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR031924-50"
FT BINDING 89
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR031924-51"
FT BINDING 152..154
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR031924-51"
SQ SEQUENCE 537 AA; 56751 MW; 86C92A1AC4EB1C30 CRC64;
MLAQGSPAPA AKVTATTPAP KLVVVISVDQ FSADLFAEYR SHFTGGLARL ASGIVFPSGY
QSHGATETCP GHSTILTGNH PANTGIIANN YFDLSVARAD KRIYCAEDET VPGTSSGSGK
YAASVNHLLV PTLGDLMKAA NPKAQVVSVA GKDRAAIMMG GRQADELMWL VPTGLTGYRG
TTLSPTAQQA SAAIAAAIAQ PRAASPLPAD CTPRDIAIPI GDKGATVGTG RFQRDGGDFR
RFMASPEADG AVLAAGAALR TARKLGEGDT ADLLILGLSA TDYVGHGTGT EGSEMCMQML
ALDRELGDFF TRLDATGIDY VVALTADHGG HDLPERNRQN AWPAAERVDK ALDPDAMGKA
VAEKLGLPQP LLYSDGPFGD MYLSKTLTPA QRKTALAEFV SRFKAHPQVE AVVLGEDLAK
YPISKRAPDT WTLMDKLRAS YNAQRSGDFI VVLKPRVTPI AESGLGYVAT HGSVWDYDRR
VPILFWRRGL TGFEQPNAVM TVDILPTLAS VIGVPLDASK IDGRCLDLLS GPESSCR
//
