ID A0A0W8JD56_9VIBR Unreviewed; 402 AA.
AC A0A0W8JD56;
DT 16-MAR-2016, integrated into UniProtKB/TrEMBL.
DT 16-MAR-2016, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=trans-2-enoyl-CoA reductase (NAD(+)) {ECO:0000256|ARBA:ARBA00011983};
DE EC=1.3.1.44 {ECO:0000256|ARBA:ARBA00011983};
GN ORFNames=VRK_28680 {ECO:0000313|EMBL:KUI98167.1};
OS Vibrio sp. MEBiC08052.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=1761910 {ECO:0000313|EMBL:KUI98167.1, ECO:0000313|Proteomes:UP000054473};
RN [1] {ECO:0000313|EMBL:KUI98167.1, ECO:0000313|Proteomes:UP000054473}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MEBiC08052 {ECO:0000313|EMBL:KUI98167.1,
RC ECO:0000313|Proteomes:UP000054473};
RA Kim Y.J., Lee J.-H., Kwon K.K.;
RT "Genome sequence of Vibrio sp. MEBiC08052.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 2,3-saturated acyl-CoA + NAD(+) = a (2E)-enoyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:18177, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540,
CC ChEBI:CHEBI:57945, ChEBI:CHEBI:58856, ChEBI:CHEBI:65111; EC=1.3.1.44;
CC Evidence={ECO:0000256|ARBA:ARBA00001615};
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SUBUNIT: Monomer. {ECO:0000256|ARBA:ARBA00011245}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KUI98167.1}.
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DR EMBL; LQIY01000022; KUI98167.1; -; Genomic_DNA.
DR RefSeq; WP_059121867.1; NZ_KQ947475.1.
DR AlphaFoldDB; A0A0W8JD56; -.
DR STRING; 1761910.VRK_28680; -.
DR PATRIC; fig|1761910.3.peg.2894; -.
DR OrthoDB; 9802260at2; -.
DR Proteomes; UP000054473; Unassembled WGS sequence.
DR GO; GO:0004318; F:enoyl-[acyl-carrier-protein] reductase (NADH) activity; IEA:UniProt.
DR GO; GO:0050343; F:trans-2-enoyl-CoA reductase (NAD+) activity; IEA:UniProtKB-EC.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR024906; Eno_Rdtase_FAD-bd_dom.
DR InterPro; IPR024910; Enoyl-CoA_Rdtase_cat_dom.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR010758; Trans-2-enoyl-CoA_reductase.
DR PANTHER; PTHR37480; ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADH]; 1.
DR PANTHER; PTHR37480:SF1; ENOYL-[ACYL-CARRIER-PROTEIN] REDUCTASE [NADH]; 1.
DR Pfam; PF07055; Eno-Rase_FAD_bd; 1.
DR Pfam; PF12242; Eno-Rase_NADH_b; 1.
DR Pfam; PF12241; Enoyl_reductase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
KW Fatty acid biosynthesis {ECO:0000256|ARBA:ARBA00023160};
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002}.
FT DOMAIN 84..320
FT /note="Trans-2-enoyl-CoA reductase catalytic"
FT /evidence="ECO:0000259|Pfam:PF12241"
FT DOMAIN 327..388
FT /note="Enoyl reductase FAD binding"
FT /evidence="ECO:0000259|Pfam:PF07055"
SQ SEQUENCE 402 AA; 44205 MW; F425A0713D7E0AC1 CRC64;
MHITPKINGV VAHSAHPYGC EQSIIQQIQY AKAATPIQHG PKRVLILGAS SGLGLAARIA
LTFGGAKADT IGVSLDTPPQ TNHSGSAGFY NNYYFKQYAQ REGRTAINIQ GDVFSRRTKE
AVIEAIETYF EGEVDLIIYS VASGKRRKAE GSDSFWYSAI KPIGQSIQSV LLDLGHDQWH
DVILEPALEE EIDSTLKVMG GEDWEAWIDA LINSESIADG CQSIAFSYIG SALTHPIYLD
GTLGRAKVDL HQTSHSLNMK LANYGGHAYA VVCQALLTRA SIFIPGLSPY LIALNQAMHA
LGVNENCNSQ MQRLFTTKLY GPETVPVDGE RLIRLDEHEL SPEVQTKTQQ LLAGINADNL
SQLDAYQTFK KAFMRLNGFQ WPAIDDHAPI DLRPFIKFAT KE
//
