ID A0A0Y0NDB3_9MICO Unreviewed; 844 AA.
AC A0A0Y0NDB3;
DT 13-APR-2016, integrated into UniProtKB/TrEMBL.
DT 13-APR-2016, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
GN ORFNames=AWU67_01355 {ECO:0000313|EMBL:AMB57731.1};
OS Microterricola viridarii.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Microterricola.
OX NCBI_TaxID=412690 {ECO:0000313|EMBL:AMB57731.1, ECO:0000313|Proteomes:UP000058305};
RN [1] {ECO:0000313|EMBL:AMB57731.1, ECO:0000313|Proteomes:UP000058305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ERGS5:02 {ECO:0000313|EMBL:AMB57731.1,
RC ECO:0000313|Proteomes:UP000058305};
RX PubMed=26854947; DOI=10.1016/j.jbiotec.2016.02.011;
RA Himanshu, Swarnkar M.K., Singh D., Kumar R.;
RT "First complete genome sequence of a species in the genus Microterricola,
RT an extremophilic cold active enzyme producing bacterial strain ERGS5:02
RT isolated from Sikkim Himalaya.";
RL J. Biotechnol. 222:17-18(2016).
RN [2] {ECO:0000313|Proteomes:UP000058305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ERGS5:02 {ECO:0000313|Proteomes:UP000058305};
RA Kumar R., Singh D., Swarnkar M.K.;
RT "First complete genome sequence of a species in the genus Microterricola,
RT an extremophilic cold active enzyme producing strain ERGS5:02 isolated from
RT Sikkim Himalaya.";
RL Submitted (JAN-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
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DR EMBL; CP014145; AMB57731.1; -; Genomic_DNA.
DR RefSeq; WP_067225737.1; NZ_CP014145.1.
DR AlphaFoldDB; A0A0Y0NDB3; -.
DR KEGG; mvd:AWU67_01355; -.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000058305; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000058305}.
FT DOMAIN 59..367
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 368..647
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT REGION 1..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 731..775
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 80..87
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 844 AA; 91360 MW; A4938CDB40ED4231 CRC64;
MSTLFDPDEP TDSRPMIVHD GPGGFPAVST PSGTPAGGPG LPGGLPGGGA WAGGDSLLDG
LNPEQREAVE YRGPALLIVA GAGSGKTRVL THRIAGLIGS REAWPSQILA ITFTNKAAAE
MRERVGALLG QAATGMWIST FHSACVRILR AEAERAGLSS TFTIYDSADS RNVLKRIIKE
LSADTLGFTA AGVSNKISKL KNELADADSY ARNANLSDPQ ELMFIEIFRQ YTRRLRAAQA
LDFDDLLAET VYLFRAFPAT AALYQRRFRH ILVDEYQDTN HAQYSFIKEL TRPIEPHIVA
ELGTAGHHVR SLQDASGAIP GASLTVVGDS DQSIYAFRGA DIRNIVEFER DYPGAKVVLL
EQNYRSTQNI LSAANAVISN NFDRKDKKLW TAVGDGAKII GYTGYSGHDE AQFIADEIEK
LHAEGVAYRD IAVFYRTNAQ TRALEEIFVR AALPYRVVGG TKFYERAEIK DAMAYLIAVA
NPADELALRR ILNTPKRGIG PATETQLASF AEANGISFRQ AMRDAGSLGM GPKVTGAILK
LAKLLDEAAL MLDPENPAGV SSVSEVLGFL LTESTLLEVL RGSRDPQDET RAENVEELLA
QTKDFVKENP DAGLIDFLTQ VSLVAAADDL DDSSGTVSLM TLHTAKGLEY DSVFLTGIEE
GLLPHQMSAS EPGGPAEERR LFYVGITRAR KRLYLSLAMT RAQFGETNVA MPSRYLQEIP
GDLIEWRQSP GMANSRGGTQ PRALNARRDG FGSRGGSGSG SERFGGELPP APKPKTEWAN
RVTAQVRDNG DLTLEAGDRI RHVDFGEGRV NQVTGEGTKR IAHVQFDTAG AKKLLIKIAP
IEKI
//
