ID A0A136NTV6_9BACT Unreviewed; 421 AA.
AC A0A136NTV6;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 24-JAN-2024, entry version 24.
DE RecName: Full=Aconitate hydratase A {ECO:0000256|ARBA:ARBA00019378};
DE EC=4.2.1.3 {ECO:0000256|ARBA:ARBA00012926};
DE AltName: Full=Iron-responsive protein-like {ECO:0000256|ARBA:ARBA00031977};
DE AltName: Full=RNA-binding protein {ECO:0000256|ARBA:ARBA00031081};
DE Flags: Fragment;
GN Name=acnA_2 {ECO:0000313|EMBL:KXK49567.1};
GN ORFNames=UZ05_CHB002001841 {ECO:0000313|EMBL:KXK49567.1};
OS Chlorobi bacterium OLB5.
OC Bacteria; Chlorobiota.
OX NCBI_TaxID=1617412 {ECO:0000313|EMBL:KXK49567.1, ECO:0000313|Proteomes:UP000070057};
RN [1] {ECO:0000313|EMBL:KXK49567.1, ECO:0000313|Proteomes:UP000070057}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=OLB5 {ECO:0000313|EMBL:KXK49567.1};
RA Speth D.R., In T Zandt M., Guerrero Cruz S., Dutilh B.E., Jetten M.S.;
RT "Genome based microbial ecology of anammox granules in a full-scale
RT wastewater treatment system.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=citrate = D-threo-isocitrate; Xref=Rhea:RHEA:10336,
CC ChEBI:CHEBI:15562, ChEBI:CHEBI:16947; EC=4.2.1.3;
CC Evidence={ECO:0000256|ARBA:ARBA00023501};
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC from oxaloacetate: step 2/2. {ECO:0000256|ARBA:ARBA00004717}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KXK49567.1}.
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DR EMBL; LLZO01000206; KXK49567.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A136NTV6; -.
DR PATRIC; fig|1617412.3.peg.1923; -.
DR UniPathway; UPA00223; UER00718.
DR Proteomes; UP000070057; Unassembled WGS sequence.
DR GO; GO:0003994; F:aconitate hydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR Gene3D; 3.30.499.10; Aconitase, domain 3; 1.
DR Gene3D; 3.20.19.10; Aconitase, domain 4; 1.
DR InterPro; IPR015931; Acnase/IPM_dHydase_lsu_aba_1/3.
DR InterPro; IPR001030; Acoase/IPM_deHydtase_lsu_aba.
DR InterPro; IPR015928; Aconitase/3IPM_dehydase_swvl.
DR InterPro; IPR018136; Aconitase_4Fe-4S_BS.
DR InterPro; IPR036008; Aconitase_4Fe-4S_dom.
DR InterPro; IPR000573; AconitaseA/IPMdHydase_ssu_swvl.
DR PANTHER; PTHR43160; ACONITATE HYDRATASE B; 1.
DR PANTHER; PTHR43160:SF3; ACONITATE HYDRATASE, MITOCHONDRIAL; 1.
DR Pfam; PF00330; Aconitase; 1.
DR Pfam; PF00694; Aconitase_C; 1.
DR SUPFAM; SSF53732; Aconitase iron-sulfur domain; 1.
DR SUPFAM; SSF52016; LeuD/IlvD-like; 1.
DR PROSITE; PS00450; ACONITASE_1; 1.
DR PROSITE; PS01244; ACONITASE_2; 1.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723}.
FT DOMAIN 15..146
FT /note="Aconitase/3-isopropylmalate dehydratase large
FT subunit alpha/beta/alpha"
FT /evidence="ECO:0000259|Pfam:PF00330"
FT DOMAIN 226..355
FT /note="Aconitase A/isopropylmalate dehydratase small
FT subunit swivel"
FT /evidence="ECO:0000259|Pfam:PF00694"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KXK49567.1"
SQ SEQUENCE 421 AA; 46746 MW; EBA9ADE6A87D8664 CRC64;
AWPISKFAQA VKEKDYPEEL KVALIGSCTN SSYEDMERSA SIAKQALAKG LKVKSEFTIT
PGSEQIRATI ERDGQLEIFE KAGGIVLANA CGPCIGQWKR HDVKEGEKNS IITSYNRNFS
KRNDGNPATN AFVASPEIVT ALAFAGRLTF NPLTDSLTNE KGEQVKLDEP KGDELPKKGY
LKGEDGYIAP AADGSSINVE IDPESKRLQR LFPFVHPDFE KDFKDMHLLL KVKGKCTTDH
ISMAGPWLRF RGHLDNISNN MFIGAVNAFN DKTNYVKNHL TGNYDEVPNA ARAYKAKNIP
WVVVGEENYG EGSSREHAAM EPRHLGGKAI IVKSFARIHE TNLKKQGMLP LTFADPADYY
KIREDDKIDL DVKDIAPGSQ VKMTLKHADG STDTVMLNHT MNKQQIDWFK VGSALNLMAK
K
//
