ID   A0A151NK84_ALLMI        Unreviewed;       867 AA.
AC   A0A151NK84;
DT   08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT   08-JUN-2016, sequence version 1.
DT   27-MAR-2024, entry version 23.
DE   RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE            EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN   Name=AREL1 {ECO:0000313|EMBL:KYO37197.1};
GN   ORFNames=Y1Q_0008906 {ECO:0000313|EMBL:KYO37197.1};
OS   Alligator mississippiensis (American alligator).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Archelosauria; Archosauria; Crocodylia; Alligatoridae; Alligatorinae;
OC   Alligator.
OX   NCBI_TaxID=8496 {ECO:0000313|EMBL:KYO37197.1};
RN   [1] {ECO:0000313|EMBL:KYO37197.1, ECO:0000313|Proteomes:UP000050525}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KSC_2009_1 {ECO:0000313|EMBL:KYO37197.1};
RX   PubMed=22293439; DOI=10.1186/gb-2012-13-1-415;
RA   St John J.A., Braun E.L., Isberg S.R., Miles L.G., Chong A.Y., Gongora J.,
RA   Dalzell P., Moran C., Bed'hom B., Abzhanov A., Burgess S.C., Cooksey A.M.,
RA   Castoe T.A., Crawford N.G., Densmore L.D., Drew J.C., Edwards S.V.,
RA   Faircloth B.C., Fujita M.K., Greenwold M.J., Hoffmann F.G., Howard J.M.,
RA   Iguchi T., Janes D.E., Khan S.Y., Kohno S., de Koning A.J., Lance S.L.,
RA   McCarthy F.M., McCormack J.E., Merchant M.E., Peterson D.G., Pollock D.D.,
RA   Pourmand N., Raney B.J., Roessler K.A., Sanford J.R., Sawyer R.H.,
RA   Schmidt C.J., Triplett E.W., Tuberville T.D., Venegas-Anaya M.,
RA   Howard J.T., Jarvis E.D., Guillette L.J.Jr., Glenn T.C., Green R.E.,
RA   Ray D.A.;
RT   "Sequencing three crocodilian genomes to illuminate the evolution of
RT   archosaurs and amniotes.";
RL   Genome Biol. 13:415-415(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC         [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC         cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC         EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:KYO37197.1}.
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DR   EMBL; AKHW03002792; KYO37197.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A151NK84; -.
DR   Proteomes; UP000050525; Unassembled WGS sequence.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR   GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR   CDD; cd00078; HECTc; 1.
DR   Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR   Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR   InterPro; IPR000569; HECT_dom.
DR   InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR   InterPro; IPR014756; Ig_E-set.
DR   PANTHER; PTHR11254:SF340; APOPTOSIS-RESISTANT E3 UBIQUITIN PROTEIN LIGASE 1; 1.
DR   PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR   Pfam; PF00632; HECT; 1.
DR   SMART; SM00119; HECTc; 1.
DR   SUPFAM; SSF81296; E set domains; 1.
DR   SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR   PROSITE; PS50237; HECT; 1.
PE   4: Predicted;
KW   Membrane {ECO:0000256|SAM:Phobius};
KW   Reference proteome {ECO:0000313|Proteomes:UP000050525};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius};
KW   Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW   ECO:0000256|PROSITE-ProRule:PRU00104}.
FT   TRANSMEM        54..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          527..867
FT                   /note="HECT"
FT                   /evidence="ECO:0000259|PROSITE:PS50237"
FT   REGION          357..390
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        834
FT                   /note="Glycyl thioester intermediate"
FT                   /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ   SEQUENCE   867 AA;  98701 MW;  B34DC44907B76AED CRC64;
     MGGTGQVPGC FADAVSLVDS IWAGTGLLHL GMECGQSCKD AAVIFGCVSH RRDLMFYIIG
     GITVSVVAFF FTIKFLFELA ARVVSFLQHE DRERRGERTI YDYVRGNYLD PRSCKVSWDW
     KDPYELFYKN GQPFPAHRPV GLRVHICHVE LAIDIPVTQE VLQEPSSNVV KVAFTVRRAG
     RYEITIKLGG LNVAYSPYYK LFQPGMVVPS KTKIVCHFST LVLTCGQKHT LQIVPRDEYD
     NPTSNSVSLI DEHNYSLSIH ELGPQEEESS DVLFEKSVMS NRQTCQVFLR LTLHRRGCFH
     ACISYQNQPI SNGDFDIIVL SENEKNIVER NVSTSGVSIY FEAYLYNATS YTNTQWHLPP
     THLGSSQRRP STATEDEDED SPSDSQTPEK VKKPKKVYCY VSPKQFSVKE FYLKIIPWRL
     FTFRVCPGTK FSYLGPDPVH KLLTLVVDDG IQPPVELSCK ERNILAATFI RSLHKNIGGS
     ETFQDKVNFF QRELRQVHMK RPHSKVTLKV SRHSLLESSL KATRNFSVSD WSKNFEVIFQ
     DEEALDWGGP RREWFELICK ALFDTTNQLF TRFSDNNQAL VHPNPGRSPH LRLKVYEFAG
     RLVGKCLYES SLGGAYKQLV RARFTRSFLA QIIGLRMHYK YFETDDPEFY KSKVCFILNN
     DVSEMELVFA EEKYSKSGQL EKVVELVTGG AQIPVTNDNK ILYLNLLAQY RLANQVREEV
     DHFLKGLNEL VPENLLAIFD ENELELLMCG TGDINVCDFK THAVVVGGSW HFREKVMRWF
     WTVVSSFTQE ELARLLQFTT GSSQLPPGGF AALCPSFQII AAPTHSTLPT AHTCFNQLCL
     PTYDSYEEMH KMLQLAISEG CEGFGML
//