ID A0A151T6K3_CAJCA Unreviewed; 793 AA.
AC A0A151T6K3;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 27-MAR-2024, entry version 22.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=KK1_017227 {ECO:0000313|EMBL:KYP62682.1};
OS Cajanus cajan (Pigeon pea) (Cajanus indicus).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Cajanus.
OX NCBI_TaxID=3821 {ECO:0000313|EMBL:KYP62682.1, ECO:0000313|Proteomes:UP000075243};
RN [1] {ECO:0000313|EMBL:KYP62682.1, ECO:0000313|Proteomes:UP000075243}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Asha {ECO:0000313|Proteomes:UP000075243};
RX PubMed=22057054; DOI=10.1038/nbt.2022;
RA Varshney R.K., Chen W., Li Y., Bharti A.K., Saxena R.K., Schlueter J.A.,
RA Donoghue M.T., Azam S., Fan G., Whaley A.M., Farmer A.D., Sheridan J.,
RA Iwata A., Tuteja R., Penmetsa R.V., Wu W., Upadhyaya H.D., Yang S.P.,
RA Shah T., Saxena K.B., Michael T., McCombie W.R., Yang B., Zhang G.,
RA Yang H., Wang J., Spillane C., Cook D.R., May G.D., Xu X., Jackson S.A.;
RT "Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop
RT of resource-poor farmers.";
RL Nat. Biotechnol. 30:83-89(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; CM003610; KYP62682.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A151T6K3; -.
DR STRING; 3821.A0A151T6K3; -.
DR OMA; VTNFECR; -.
DR Proteomes; UP000075243; Chromosome 8.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR000626; Ubiquitin-like_dom.
DR InterPro; IPR029071; Ubiquitin-like_domsf.
DR InterPro; IPR019956; Ubiquitin_dom.
DR PANTHER; PTHR11254:SF424; E3 UBIQUITIN-PROTEIN LIGASE UPL5; 1.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR Pfam; PF00632; HECT; 1.
DR Pfam; PF00240; ubiquitin; 1.
DR PRINTS; PR00348; UBIQUITIN.
DR SMART; SM00119; HECTc; 1.
DR SMART; SM00213; UBQ; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR SUPFAM; SSF54236; Ubiquitin-like; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50053; UBIQUITIN_2; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000075243};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 6..82
FT /note="Ubiquitin-like"
FT /evidence="ECO:0000259|PROSITE:PS50053"
FT DOMAIN 454..793
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 759
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:KYP62682.1"
SQ SEQUENCE 793 AA; 90943 MW; 1EDECDC43FF31561 CRC64;
RPMTQKQFFV RPLPAGRSMV MTGYPEETVG SVLYRIEYAT RVPMAEQRLI YKGKQLQLDK
TLEECGIEND SNLHLVAYLR SGGHPAASRI IDYMVAVLSH VRRGDTVRYD LRIIKVLVSR
HFLVPGFAEL FMDSKAPMLL ASLYTSPPTG GDRDYAGYCI SYIMNCCRAK NLQGVSVAAA
ALEFCKQLKK FGCGCEDNMY SSCRSTLGFL LQGTGNGSAK RTVSFGDVAS FVPELVDRLI
LGLNWSMKCP TTLTKPLVVE IVDFGVFVAT VRRRVMVDQG LEEVLSDEIE KCNAGEMHEV
KGQLFEEHVD FLRIAFVRLL SKMSECFHVM AQRLADKEHE RSNVVGNIAW GKYLRILKEL
YEISKVFHDA EEQFWRILMY HRNMLSALIV GFVNRGFDHQ WILANKNVTN FECRRHLAML
QFPCAEEDYE LFQEMLIDRS KLLAESFEYI GRAEPETLRS GLFMEFKNEQ ATGPGVTREW
FVLVCHEIFN PQNALFVACP NDCRRFYPNP ASKVHPRHLE YFRFAGRIIA LALMKKVQVG
IVFDRVFFMQ LAGNNVILED IRDADPFLYR SCKLILEMHA DFIDSDALGL TFVREVEVLG
CRKVVELCPD GKSLVVNSKN REKYVDLLIK NCFVTSISDQ VSHFAKGFAD ILSNSKLEQF
FQWLELEDLD WMLHGSENTI SVDDWKAHTK YKGYKETDHQ ISWFWEIVGR MSAKQRKDLL
FFWTSVRYLP VEGFRGLASH LSICRSVQPD NHLPTSHTCF FELCFPRYSS MAVMQDRLEV
ITQEHIGLSF GTG
//
