ID A0A160KVN5_9MICO Unreviewed; 701 AA.
AC A0A160KVN5;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 24-JAN-2024, entry version 23.
DE RecName: Full=Beta-galactosidase {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
DE Short=Beta-gal {ECO:0000256|PIRNR:PIRNR001084};
DE EC=3.2.1.23 {ECO:0000256|ARBA:ARBA00012756, ECO:0000256|PIRNR:PIRNR001084};
GN ORFNames=A6122_2328 {ECO:0000313|EMBL:AND17448.1};
OS Rathayibacter tritici.
OC Bacteria; Actinomycetota; Actinomycetes; Micrococcales; Microbacteriaceae;
OC Rathayibacter.
OX NCBI_TaxID=33888 {ECO:0000313|EMBL:AND17448.1, ECO:0000313|Proteomes:UP000077071};
RN [1] {ECO:0000313|EMBL:AND17448.1, ECO:0000313|Proteomes:UP000077071}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCPPB 1953 {ECO:0000313|EMBL:AND17448.1,
RC ECO:0000313|Proteomes:UP000077071};
RA Park J., Lee H.-H., Lee S.-W., Seo Y.-S.;
RT "Complete genome sequence of Rathayibacter tritici NCPPB 1953.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-galactose residues
CC in beta-D-galactosides.; EC=3.2.1.23;
CC Evidence={ECO:0000256|ARBA:ARBA00001412,
CC ECO:0000256|PIRNR:PIRNR001084};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 42 family.
CC {ECO:0000256|ARBA:ARBA00005940, ECO:0000256|PIRNR:PIRNR001084}.
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DR EMBL; CP015515; AND17448.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A160KVN5; -.
DR STRING; 33888.A6122_2328; -.
DR KEGG; rtn:A6122_2328; -.
DR PATRIC; fig|33888.3.peg.2593; -.
DR Proteomes; UP000077071; Chromosome.
DR GO; GO:0009341; C:beta-galactosidase complex; IEA:InterPro.
DR GO; GO:0004565; F:beta-galactosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd03143; A4_beta-galactosidase_middle_domain; 1.
DR Gene3D; 3.40.50.880; -; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013738; Beta_galactosidase_Trimer.
DR InterPro; IPR029062; Class_I_gatase-like.
DR InterPro; IPR003476; Glyco_hydro_42.
DR InterPro; IPR013529; Glyco_hydro_42_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR36447; BETA-GALACTOSIDASE GANA; 1.
DR PANTHER; PTHR36447:SF2; BETA-GALACTOSIDASE YESZ; 1.
DR Pfam; PF02449; Glyco_hydro_42; 1.
DR Pfam; PF08532; Glyco_hydro_42M; 1.
DR PIRSF; PIRSF001084; B-galactosidase; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF52317; Class I glutamine amidotransferase-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|PIRNR:PIRNR001084};
KW Hydrolase {ECO:0000256|PIRNR:PIRNR001084};
KW Reference proteome {ECO:0000313|Proteomes:UP000077071}.
FT DOMAIN 17..395
FT /note="Glycoside hydrolase family 42 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF02449"
FT DOMAIN 407..614
FT /note="Beta-galactosidase trimerisation"
FT /evidence="ECO:0000259|Pfam:PF08532"
FT ACT_SITE 152
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT ACT_SITE 318
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-1"
FT BINDING 113
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
FT BINDING 151
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001084-2"
SQ SEQUENCE 701 AA; 78039 MW; FA3BA8A3BDDB3185 CRC64;
MMPAAPRFSG VLFGAAYYVE YQPEGVLERD LDLMAEAGFT VIRVGESVWS TWEPREGVFD
LDWLEPVLDA AHARGISVIL GTPTYAIPPW LQRLHPEIAA ESRTGVRMGW GARQEMDQSH
PAYRFYADRI ARKVVARYAD HPAIIGYQVD NEPGNHLPHN EHTFQRFVGW LRERYGTVED
LNREWGLVYW SHRLSDWADL WRPDGNLMPQ YQLEWRRFQS TLANELIAWQ ADLVREYARE
DQFVTTCIAY SQAQISDDEI VAPLDVVAGN PYYKMQGDLD ATAEVPRERP WWGTGVWALF
EWGDRAFSSA QTQYLVTETN AQSINGSWTN HPPYPGQIKQ AALALLARGG RMIEYWHWHT
LHFGIETYWG GVLPHSQQPG RIYREVADLG ATLKALGPAL DEYEPDADVL MLYSTDTKWS
FEFTPPLARA DGSPDSRAYL TIFDAFYRGL FEAGAQIRVQ HLRQFVASDI TELVRRYPVL
IAPAVYVADD AMLEHLRAYA EAGGHLVLGI RTGYADELAR ARLAVAPAFL TDAAGVHYDE
YSNIDAPMMV HAEGDLALEE GAAGTEWVDV LEVDDADVLL RYEPTELEAD AAVTTHAFGA
GRVSSVGTVP NPALGRSIGR WLVPDTQKSQ WHAGDSVSVS TGTTGHSRVV FLSNWSAAAT
SVTTPAPARD LVTGATYAAG DSVPLDRRGA AVLEIALDAT E
//
