ID A0A180GQV5_PUCT1 Unreviewed; 1205 AA.
AC A0A180GQV5;
DT 07-SEP-2016, integrated into UniProtKB/TrEMBL.
DT 07-SEP-2016, sequence version 1.
DT 27-MAR-2024, entry version 30.
DE RecName: Full=RNA helicase {ECO:0000256|ARBA:ARBA00012552};
DE EC=3.6.4.13 {ECO:0000256|ARBA:ARBA00012552};
GN ORFNames=PTTG_03386 {ECO:0000313|EMBL:OAV94343.1};
OS Puccinia triticina (isolate 1-1 / race 1 (BBBD)) (Brown leaf rust fungus).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina;
OC Pucciniomycetes; Pucciniales; Pucciniaceae; Puccinia.
OX NCBI_TaxID=630390 {ECO:0000313|EMBL:OAV94343.1};
RN [1] {ECO:0000313|EMBL:OAV94343.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1-1 BBBD Race 1 {ECO:0000313|EMBL:OAV94343.1};
RG The Broad Institute Genome Sequencing Platform;
RA Ward D., Feldgarden M., Earl A., Young S.K., Zeng Q., Koehrsen M.,
RA Alvarado L., Berlin A., Bochicchio J., Borenstein D., Chapman S.B.,
RA Chen Z., Engels R., Freedman E., Gellesch M., Goldberg J., Griggs A.,
RA Gujja S., Heilman E., Heiman D., Hepburn T., Howarth C., Jen D., Larson L.,
RA Lewis B., Mehta T., Park D., Pearson M., Roberts A., Saif S., Shea T.,
RA Shenoy N., Sisk P., Stolte C., Sykes S., Thomson T., Walk T., White J.,
RA Yandava C., Izard J., Baranova O.V., Blanton J.M., Tanner A.C.,
RA Dewhirst F.E., Haas B., Nusbaum C., Birren B.;
RL Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OAV94343.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1-1 BBBD Race 1 {ECO:0000313|EMBL:OAV94343.1};
RA Cuomo C.A., Bakkeren G., Szabo L., Khalil H., Joly D., Goldberg J.,
RA Young S., Zeng Q., Fellers J.;
RT "Comparative analysis highlights variable genome content of wheat rusts and
RT divergence of the mating loci.";
RL Submitted (MAY-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00001556};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAV94343.1}.
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DR EMBL; ADAS02000040; OAV94343.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A180GQV5; -.
DR STRING; 630390.A0A180GQV5; -.
DR VEuPathDB; FungiDB:PTTG_03386; -.
DR OrthoDB; 3682876at2759; -.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-KW.
DR GO; GO:1990904; C:ribonucleoprotein complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR GO; GO:0008380; P:RNA splicing; IEA:UniProtKB-KW.
DR CDD; cd21691; GH2-like_DHX8; 1.
DR CDD; cd05684; S1_DHX8_helicase; 1.
DR CDD; cd18791; SF2_C_RHA; 1.
DR Gene3D; 1.20.120.1080; -; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR011709; DEAD-box_helicase_OB_fold.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR049588; DHX8_GH2-like.
DR InterPro; IPR002464; DNA/RNA_helicase_DEAH_CS.
DR InterPro; IPR048333; HA2_WH.
DR InterPro; IPR007502; Helicase-assoc_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR049621; S1_DHX8_helicase.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR18934; ATP-DEPENDENT RNA HELICASE; 1.
DR PANTHER; PTHR18934:SF85; ATP-DEPENDENT RNA HELICASE DHX8; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF21010; HA2_C; 1.
DR Pfam; PF04408; HA2_N; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF07717; OB_NTP_bind; 1.
DR Pfam; PF00575; S1; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM00847; HA2; 1.
DR SMART; SM00490; HELICc; 1.
DR SMART; SM00316; S1; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS00690; DEAH_ATP_HELICASE; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
DR PROSITE; PS50126; S1; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741}.
FT DOMAIN 241..311
FT /note="S1 motif"
FT /evidence="ECO:0000259|PROSITE:PS50126"
FT DOMAIN 551..714
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 739..912
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 83..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 160..233
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 462..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 85..108
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 160..225
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..476
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1205 AA; 135087 MW; 56CC9F6CE6F64E69 CRC64;
MDQEFEKLEI LSLVNSITKE LHNHVGLQDS TLAEYLISLH EESTSFENFK LKLQEIGAEF
PESFITNMDR LVTSLHPKYK KKKLPAEGKG KEKDIDPAED EEELERKKRR AQALFPALAL
PDRSWEPSFV PDPKDATAKN TSTADQLLEE LEHVGRRHRE LQAVREEEQE DDASSDYNRP
TKMPRRDRSA SRERGRRSPE PRSGRFRDDY SGGRFDDRGR QRDNGYGNRA SKAPDTRPVL
YKIYDGTVAS IKDFGCFVTL KGVAGKAEGL VHVNAIAAGR VNHPSDLVSR GQPVKVKVVS
IAGERIGISM KDVDQNTGAD LTPHLRIKSD AEMAEEEAQY AARHVNHSVG AGFADDNRSS
SARRLTSPER WEIKQLIASG AASAADYPNL DDDLTTPGST NGMAAAATAE EELDIEMRED
EAPFLKGAHR RVLDLSPVKI VKAPDGTLNR AALAGAGLAK ERRELRQQEA NERADAETQD
TSTAWLDPVA KPHERLFAQD ARDNTMGKKQ EESGWKQATF NQATTYGKIT SLSITEQRAS
LPIYKLRDAL VKAVKENQIL VVVGDTGSGK TTQMTQYLAE EGLADEKKIA CTQPRRVAAM
SVAKRVAEEV GCRLGQDVGY TIRFEDCTSS ETKIKYMTDG MLQREALVDP NLSAYSVIML
DEAHERTIAT DVLFGLLKKS IMRRPDLKLI VTSATLDAEK FSKYFYSCPI FTIPGRTYPV
EVLYTKEPES DYLDAALITI MQIHISEPPG DILLFLTGQE EIDTSAEILY ERMKALGSHV
PELIVLPVYS ALPSEMQSKI FDPAPPGARK VILATNIAET SITIDGIYYV VDPGFVKQKA
WDPRLGMDSL VVTPISQAQA RQRSGRAGRT GPGKCYRLYT EAAYRNEMLP TSIPDIQRQN
LAHTILMLKA MGINDLLNFD FMDPPPQQTM ITALENLYAL SALDDEGLLT RLGRKMADFP
MDPELSKMLI ASVDLGCSEE VLTIVAMISG ATNVFYRPKE KQAQADAKKA KFHQPEGDHL
TLLAVYDGWK NNKFSNPWCH ENYIQSRAMR RAQDVRKQLL GIMDRYKHDI VSCGTNYDRV
RRAICSGYFR HAAKKDPQEG YKTLVEGTPV FIHPSSALFN RAPEWIIYHE LVLTTKEYCR
DVTAIEPKWL TEVAPTFFKV ADAKTMSKRK RNERVQPLFD RFAKSENEWR ISKVKRSTRT
SQTFG
//
