ID MEP_NEOFI Reviewed; 634 AA.
AC A1DBW0;
DT 05-APR-2011, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 1.
DT 22-FEB-2023, entry version 79.
DE RecName: Full=Extracellular metalloproteinase MEP;
DE EC=3.4.24.-;
DE AltName: Full=Elastinolytic metalloproteinase MEP;
DE AltName: Full=Fungalysin MEP;
DE Flags: Precursor;
GN Name=MEP; ORFNames=NFIA_099860;
OS Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164
OS / JCM 1740 / NRRL 181 / WB 181) (Aspergillus fischerianus).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Fumigati.
OX NCBI_TaxID=331117;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181
RC / WB 181;
RX PubMed=18404212; DOI=10.1371/journal.pgen.1000046;
RA Fedorova N.D., Khaldi N., Joardar V.S., Maiti R., Amedeo P., Anderson M.J.,
RA Crabtree J., Silva J.C., Badger J.H., Albarraq A., Angiuoli S., Bussey H.,
RA Bowyer P., Cotty P.J., Dyer P.S., Egan A., Galens K., Fraser-Liggett C.M.,
RA Haas B.J., Inman J.M., Kent R., Lemieux S., Malavazi I., Orvis J.,
RA Roemer T., Ronning C.M., Sundaram J.P., Sutton G., Turner G., Venter J.C.,
RA White O.R., Whitty B.R., Youngman P., Wolfe K.H., Goldman G.H.,
RA Wortman J.R., Jiang B., Denning D.W., Nierman W.C.;
RT "Genomic islands in the pathogenic filamentous fungus Aspergillus
RT fumigatus.";
RL PLoS Genet. 4:E1000046-E1000046(2008).
CC -!- FUNCTION: Secreted metalloproteinase that allows assimilation of
CC proteinaceous substrates and probably acts as a virulence factor.
CC {ECO:0000250}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- INDUCTION: Expression is controlled by the prtT transcription factor.
CC -!- SIMILARITY: Belongs to the peptidase M36 family. {ECO:0000305}.
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DR EMBL; DS027694; EAW20350.1; -; Genomic_DNA.
DR RefSeq; XP_001262247.1; XM_001262246.1.
DR AlphaFoldDB; A1DBW0; -.
DR SMR; A1DBW0; -.
DR STRING; 331117.A1DBW0; -.
DR MEROPS; M36.001; -.
DR EnsemblFungi; EAW20350; EAW20350; NFIA_099860.
DR GeneID; 4588767; -.
DR KEGG; nfi:NFIA_099860; -.
DR VEuPathDB; FungiDB:NFIA_099860; -.
DR eggNOG; ENOG502QTDC; Eukaryota.
DR HOGENOM; CLU_012703_3_0_1; -.
DR OMA; IRKDSYT; -.
DR OrthoDB; 2786251at2759; -.
DR Proteomes; UP000006702; Unassembled WGS sequence.
DR GO; GO:0005615; C:extracellular space; IEA:InterPro.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09596; M36; 1.
DR Gene3D; 3.10.170.10; -; 1.
DR Gene3D; 1.10.390.10; Neutral Protease Domain 2; 1.
DR InterPro; IPR011096; FTP_domain.
DR InterPro; IPR001842; Peptidase_M36.
DR InterPro; IPR027268; Peptidase_M4/M1_CTD_sf.
DR PANTHER; PTHR33478; EXTRACELLULAR METALLOPROTEINASE MEP; 1.
DR PANTHER; PTHR33478:SF1; EXTRACELLULAR METALLOPROTEINASE MEP; 1.
DR Pfam; PF07504; FTP; 1.
DR Pfam; PF02128; Peptidase_M36; 1.
DR PRINTS; PR00999; FUNGALYSIN.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
DR PROSITE; PS00142; ZINC_PROTEASE; 1.
PE 2: Evidence at transcript level;
KW Hydrolase; Metal-binding; Metalloprotease; Protease; Reference proteome;
KW Secreted; Signal; Virulence; Zinc; Zymogen.
FT SIGNAL 1..18
FT /evidence="ECO:0000255"
FT PROPEP 19..245
FT /evidence="ECO:0000250"
FT /id="PRO_0000407189"
FT CHAIN 246..634
FT /note="Extracellular metalloproteinase MEP"
FT /id="PRO_0000407190"
FT ACT_SITE 430
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 429
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
FT BINDING 433
FT /ligand="Zn(2+)"
FT /ligand_id="ChEBI:CHEBI:29105"
FT /ligand_note="catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU10095"
SQ SEQUENCE 634 AA; 68721 MW; D3416CD05C214970 CRC64;
MRGLLLAGAL ALPASVFAHP AHQSYGLNRR TVDLNAFRLK SLAKYVNATE TVIEAPSSFA
PFKQQSYVEA ATQHVKMIAP DATFRVVDDH YVGDNGVAHV HFRQTANGLD IDNADFNVNV
GKDGKVFSYG NSFYTGQIPS SAALTKRDFS DPVTALKGTT NTLQLPITVD SASSESTEGK
ESYVFKGVSG TVSDPKANLV YFVKDDGTLA LTWRVETDID SNWLLTYIDA KSGEQIHGVV
DYVAEADYQV YAWGINDPTE GERTVVKDPW DSAASEFTWI SDGSTKYTTS RGNNGIAQSN
PNGGSSYLNN YRPSSSSLSF KYPYSPSSSP PSSYIDASII QLFYTANTYH DLLYTLGFNE
KAGNFEYNTN GQGGRGNDYV ILNAQDGSGT NNANFATPPD GQPGRMRMYV WTESTPYRDG
SFEAGIVIHE YTHGLSTRLT GGPANSNCLN ALESGGMGEG WGDFMATAIR LKPGDKRSTD
YTMGEWAANR PGGIRQYPYS TSMSTNPLTY TSVNSLNAVH AIGTVWATML YEVMWNLIDK
HGKNDAPKPT LRDGVPTDGK YLAMKLVIDG MALQPCNPNF VQARDAILDA DTALTGGENQ
CEIWKAFAKR GLGAGAQYSS RNRVGSTEVP SGVC
//
