ID A1L2H2_XENLA Unreviewed; 913 AA.
AC A1L2H2;
DT 06-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 06-FEB-2007, sequence version 1.
DT 27-MAR-2024, entry version 88.
DE RecName: Full=hexokinase {ECO:0000256|ARBA:ARBA00012324};
DE EC=2.7.1.1 {ECO:0000256|ARBA:ARBA00012324};
GN Name=hk2.L {ECO:0000313|RefSeq:NP_001090603.1,
GN ECO:0000313|Xenbase:XB-GENE-5817417};
GN Synonyms=hk2 {ECO:0000313|RefSeq:NP_001090603.1,
GN ECO:0000313|Xenbase:XB-GENE-5817417}, LOC100036846
GN {ECO:0000313|EMBL:AAI29527.1};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355 {ECO:0000313|EMBL:AAI29527.1};
RN [1] {ECO:0000313|RefSeq:NP_001090603.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=12454917; DOI=10.1002/dvdy.10174;
RA Klein S.L., Strausberg R.L., Wagner L., Pontius J., Clifton S.W.,
RA Richardson P.;
RT "Genetic and genomic tools for Xenopus research: The NIH Xenopus
RT initiative.";
RL Dev. Dyn. 225:384-391(2002).
RN [2] {ECO:0000313|EMBL:AAI29527.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Spleen {ECO:0000313|EMBL:AAI29527.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|RefSeq:NP_001090603.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-glucose = ADP + D-glucose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:17825, ChEBI:CHEBI:4167, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61548, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:17826;
CC Evidence={ECO:0000256|ARBA:ARBA00000435};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + D-hexose = ADP + D-hexose 6-phosphate + H(+);
CC Xref=Rhea:RHEA:22740, ChEBI:CHEBI:4194, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61567, ChEBI:CHEBI:456216; EC=2.7.1.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:22741;
CC Evidence={ECO:0000256|ARBA:ARBA00000417};
CC -!- PATHWAY: Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-
CC phosphate and glycerone phosphate from D-glucose: step 1/4.
CC {ECO:0000256|ARBA:ARBA00004888}.
CC -!- PATHWAY: Carbohydrate metabolism; hexose metabolism.
CC {ECO:0000256|ARBA:ARBA00005028}.
CC -!- SIMILARITY: Belongs to the hexokinase family.
CC {ECO:0000256|ARBA:ARBA00009225}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; BC129526; AAI29527.1; -; mRNA.
DR RefSeq; NP_001090603.1; NM_001097134.1.
DR DNASU; 100036846; -.
DR GeneID; 100036846; -.
DR KEGG; xla:100036846; -.
DR AGR; Xenbase:XB-GENE-5817417; -.
DR CTD; 100036846; -.
DR Xenbase; XB-GENE-5817417; hk2.L.
DR OrthoDB; 5305217at2759; -.
DR UniPathway; UPA00109; UER00180.
DR UniPathway; UPA00242; -.
DR Proteomes; UP000186698; Chromosome 3L.
DR Bgee; 100036846; Expressed in spleen and 19 other cell types or tissues.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005741; C:mitochondrial outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0005536; F:glucose binding; IEA:InterPro.
DR GO; GO:0004396; F:hexokinase activity; IEA:UniProtKB-EC.
DR GO; GO:0006096; P:glycolytic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0019318; P:hexose metabolic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0001678; P:intracellular glucose homeostasis; IEA:InterPro.
DR CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 2.
DR Gene3D; 3.30.420.40; -; 2.
DR Gene3D; 3.40.367.20; -; 2.
DR InterPro; IPR043129; ATPase_NBD.
DR InterPro; IPR001312; Hexokinase.
DR InterPro; IPR019807; Hexokinase_BS.
DR InterPro; IPR022673; Hexokinase_C.
DR InterPro; IPR022672; Hexokinase_N.
DR PANTHER; PTHR19443; HEXOKINASE; 1.
DR PANTHER; PTHR19443:SF1; HEXOKINASE-3; 1.
DR Pfam; PF00349; Hexokinase_1; 2.
DR Pfam; PF03727; Hexokinase_2; 2.
DR PRINTS; PR00475; HEXOKINASE.
DR SUPFAM; SSF53067; Actin-like ATPase domain; 4.
DR PROSITE; PS00378; HEXOKINASE_1; 2.
DR PROSITE; PS51748; HEXOKINASE_2; 2.
PE 2: Evidence at transcript level;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Glycolysis {ECO:0000256|ARBA:ARBA00023152};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000186698};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 20..215
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 222..453
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
FT DOMAIN 464..661
FT /note="Hexokinase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00349"
FT DOMAIN 667..901
FT /note="Hexokinase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF03727"
SQ SEQUENCE 913 AA; 99813 MW; 3B7D4656B5996153 CRC64;
MVSPKGDQTH NDQQARHEQI ESCLQPFHLS QAQLLSVRDL MLGDMERGLM KTPQKPSTLK
MLPTYVRSTP DGTERGDFLV LDLGGAQFRI LHVELKDSAK VEMESQVYAI PDEVMHGTGQ
QLFDHVAECL GQFLRQQKLH QKVLPLGFTF SFPCVQSQLD KSVLISWTKG FHCSEVEGKD
VVHLFREAIR RQGGYDVDVI AIVNDTVGAM MSCGYQDHSC EVGFIVGTGT NLCYMEEMGN
VESVEGDEGK MCINTEWGAL GDDGELSSVQ TEVDEQVDQN SLVPGKQCFD KLVSGMYMGE
IVRLILVKLA NQGLLFDGTT SQSLLTKGCI ETKHVSAIED DHFGLAAARK MLSVLGLNPS
EQDCVNVQHV CSAVSTRSAN LCAAGLAAVA TRLQLNNHNL KITIGVDGSV YKQHPKFSER
LHSALQHLAP ECQIKFLISE DGSGKGTAIV TAVAKRLAAQ RHQIDDILAP FQLSQAKLEE
VKSQMRAEID IGLCKETHAG ATVKMLPTYV RSTPDGTERG DFIALDLGGT NFRVLYVHIG
RREDAGVQIV SKTYTLPQEI IQGTGGQLFD HIIDCISEFQ SENNLRGRRL PLGFTFSFPC
KQTNLDQGIL ITWTKGFSAS GCVGKDVVTL LREAALRKKN NDIIVVALVN DTVGTMMSCG
YNDPACEIGL IVGTGTNACY MEELKNVELL DGDEGQMCVN MEWGAFGDNN CLEDITTSFD
HDVDTFSINP GKQRYEKMIS GMYLGEIVRQ ILIVLTRRGI LFGGKISERL LTRDLFPTRF
LSLIESDTLG LVQVRSILTE LGLRSTCDDT MLVKEVCTTV SRRAAQLCAA GVAAVVEKMR
ANRGLDQLKV TVGVDGTLYK LHPHFAGVVQ ETVKILAPKC DVTFLQSDDG SGRGAALITA
VACRIAGAEA RAA
//
