ID A2IC68_ECOLX Unreviewed; 227 AA.
AC A2IC68;
DT 20-FEB-2007, integrated into UniProtKB/TrEMBL.
DT 20-FEB-2007, sequence version 1.
DT 03-MAY-2023, entry version 59.
DE SubName: Full=FimH {ECO:0000313|EMBL:ABM67742.1};
DE Flags: Fragment;
GN Name=fimH {ECO:0000313|EMBL:ABM67742.1};
OS Escherichia coli.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=562 {ECO:0000313|EMBL:ABM67742.1};
RN [1] {ECO:0000313|EMBL:ABM67742.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=ECOR37 {ECO:0000313|EMBL:ABM67742.1};
RA Barl T., Dobrindt U., Hacker J., Bachmann T.T.;
RT "Genotyping pathoadaptive mutations in the type 1 fimbriae binding-subunit
RT of extraintestinal pathogenic Escherichia coli with a DNA microarray.";
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0007829|PDB:4BUQ}
RP X-RAY CRYSTALLOGRAPHY (2.20 ANGSTROMS) OF 10-167, AND DISULFIDE BONDS.
RX PubMed=24900609; DOI=10.1021/ML400269V;
RA Roos G., Wellens A., Touaibia M., Yamakawa N., Geerlings P., Roy R.,
RA Wyns L., Bouckaert J.;
RT "Validation of Reactivity Descriptors to Assess the Aromatic Stacking
RT within the Tyrosine Gate of FimH.";
RL ACS Med. Chem. Lett. 4:1085-1090(2013).
RN [3] {ECO:0007829|PDB:3ZPD}
RP STRUCTURE BY NMR OF 10-167, AND DISULFIDE BONDS.
RX PubMed=24476493; DOI=10.1021/JM401666C;
RA Vanwetswinkel S., Volkov A.N., Sterckx Y.G., Garcia-Pino A., Buts L.,
RA Vranken W.F., Bouckaert J., Roy R., Wyns L., van Nuland N.A.;
RT "Study of the structural and dynamic effects in the FimH adhesin upon
RT alpha-d-heptyl mannose binding.";
RL J. Med. Chem. 57:1416-1427(2014).
RN [4] {ECO:0007829|PDB:4CA4}
RP X-RAY CRYSTALLOGRAPHY (1.42 ANGSTROMS) OF 10-167, AND DISULFIDE BONDS.
RX PubMed=28250938; DOI=10.1107/S2052252516016675;
RA Rabbani S., Krammer E.M., Roos G., Zalewski A., Preston R., Eid S.,
RA Zihlmann P., Prevost M., Lensink M.F., Thompson A., Ernst B., Bouckaert J.;
RT "Mutation of Tyr137 of the universal <i>Escherichia coli</i> fimbrial
RT adhesin FimH relaxes the tyrosine gate prior to mannose binding.";
RL IUCrJ 4:7-23(2017).
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DR EMBL; EF192355; ABM67742.1; -; Genomic_DNA.
DR PDB; 3ZPD; NMR; -; A=10-167.
DR PDB; 4BUQ; X-ray; 2.20 A; A/B=10-167.
DR PDB; 4CA4; X-ray; 2.84 A; A/B=10-167.
DR PDBsum; 3ZPD; -.
DR PDBsum; 4BUQ; -.
DR PDBsum; 4CA4; -.
DR AlphaFoldDB; A2IC68; -.
DR BMRB; A2IC68; -.
DR SMR; A2IC68; -.
DR UniLectin; A2IC68; -.
DR GO; GO:0009289; C:pilus; IEA:InterPro.
DR GO; GO:0007155; P:cell adhesion; IEA:InterPro.
DR CDD; cd10466; FimH_man-bind; 1.
DR Gene3D; 2.60.40.1090; Fimbrial-type adhesion domain; 2.
DR InterPro; IPR036937; Adhesion_dom_fimbrial_sf.
DR InterPro; IPR008966; Adhesion_dom_sf.
DR InterPro; IPR015243; FimH_man-bd.
DR Pfam; PF09160; FimH_man-bind; 1.
DR SUPFAM; SSF49401; Bacterial adhesins; 2.
PE 1: Evidence at protein level;
KW 3D-structure {ECO:0007829|PDB:3ZPD, ECO:0007829|PDB:4BUQ}.
FT DOMAIN 12..156
FT /note="FimH mannose-binding"
FT /evidence="ECO:0000259|Pfam:PF09160"
FT DISULFID 12..53
FT /evidence="ECO:0007829|PDB:3ZPD, ECO:0007829|PDB:4BUQ"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:ABM67742.1"
FT NON_TER 227
FT /evidence="ECO:0000313|EMBL:ABM67742.1"
SQ SEQUENCE 227 AA; 24042 MW; 9F79789AE06CCC08 CRC64;
MGWSVNAWSF ACKTANGTAI PIGGGSANVY VNLAPVVNVG QNLVVDLSTQ IFCHNDYPET
ITDYVTLQRG SAYGGVLSNF SGTVKYSGSS YPFPTTSETP RVVYNSRTDK PWPVALYLTP
VSSAGGVAIK AGSLIAVLIL RQTNNYNSDD FQFVWNIYAN NDVVVPTGGC DVSARDVTVT
LPDYPGSVPI PLTVYCAKSQ NLGYYLSGTT ADAGNSIFTN TASFSPA
//
