ID A2WF41_9BURK Unreviewed; 467 AA.
AC A2WF41; A0A0D5IW88;
DT 20-MAR-2007, integrated into UniProtKB/TrEMBL.
DT 20-MAR-2007, sequence version 1.
DT 27-MAR-2024, entry version 58.
DE RecName: Full=Trehalose-6-phosphate synthase {ECO:0000256|ARBA:ARBA00018539, ECO:0000256|RuleBase:RU362045};
DE EC=2.4.1.15 {ECO:0000256|ARBA:ARBA00012538, ECO:0000256|RuleBase:RU362045};
DE AltName: Full=Osmoregulatory trehalose synthesis protein A {ECO:0000256|RuleBase:RU362045};
DE AltName: Full=UDP-glucose-glucosephosphate glucosyltransferase {ECO:0000256|RuleBase:RU362045};
GN ORFNames=BDAG_03388 {ECO:0000313|EMBL:EAY70587.1};
OS Burkholderia dolosa AU0158.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Burkholderia; Burkholderia cepacia complex.
OX NCBI_TaxID=350701 {ECO:0000313|EMBL:EAY70587.1, ECO:0000313|Proteomes:UP000053482};
RN [1] {ECO:0000313|Proteomes:UP000053482}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AU0158 {ECO:0000313|Proteomes:UP000053482};
RG The Broad Institute Genome Sequencing Platform;
RA Birren B., Lander E., Galagan J., Devon K., Nusbaum C., Borowsky M.L.,
RA Jaffe D., Butler J., Alvarez P., Gnerre S., Grabherr M., Kleber M.,
RA Mauceli E., Brockman W., Rounsley S., Young S., LaButti K., Pushparaj V.,
RA DeCaprio D., Crawford M., Koehrsen M., Engels R., Montgomery P.,
RA Pearson M., Howarth C., Kodira C., Zeng Q., Yandava C., Oleary S.,
RA Alvarado L., Lory S., LiPuma J.;
RT "The Genome Sequence of Burkholderia dolosa (strain AU0158).";
RL Submitted (DEC-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probably involved in the osmoprotection via the biosynthesis
CC of trehalose. Catalyzes the transfer of glucose from UDP-alpha-D-
CC glucose (UDP-Glc) to D-glucose 6-phosphate (Glc-6-P) to form trehalose-
CC 6-phosphate. Acts with retention of the anomeric configuration of the
CC UDP-sugar donor. {ECO:0000256|RuleBase:RU362045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-glucose 6-phosphate + UDP-alpha-D-glucose = alpha,alpha-
CC trehalose 6-phosphate + H(+) + UDP; Xref=Rhea:RHEA:18889,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58429,
CC ChEBI:CHEBI:58885, ChEBI:CHEBI:61548; EC=2.4.1.15;
CC Evidence={ECO:0000256|ARBA:ARBA00001516,
CC ECO:0000256|RuleBase:RU362045};
CC -!- PATHWAY: Glycan biosynthesis; trehalose biosynthesis.
CC {ECO:0000256|ARBA:ARBA00005199, ECO:0000256|RuleBase:RU362045}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881,
CC ECO:0000256|RuleBase:RU362045}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 20 family.
CC {ECO:0000256|ARBA:ARBA00008799, ECO:0000256|RuleBase:RU362045}.
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DR EMBL; CH482381; EAY70587.1; -; Genomic_DNA.
DR RefSeq; WP_006765986.1; NZ_CP009793.1.
DR AlphaFoldDB; A2WF41; -.
DR GeneID; 64458113; -.
DR PATRIC; fig|350701.8.peg.4341; -.
DR HOGENOM; CLU_002351_7_1_4; -.
DR OrthoDB; 9815690at2; -.
DR UniPathway; UPA00299; -.
DR Proteomes; UP000053482; Unassembled WGS sequence.
DR GO; GO:0003825; F:alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity; IEA:UniProtKB-EC.
DR GO; GO:0005992; P:trehalose biosynthetic process; IEA:UniProtKB-UniPathway.
DR CDD; cd03788; GT20_TPS; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR InterPro; IPR001830; Glyco_trans_20.
DR InterPro; IPR012766; Trehalose_OtsA.
DR NCBIfam; TIGR02400; trehalose_OtsA; 1.
DR PANTHER; PTHR10788:SF106; BCDNA.GH08860; 1.
DR PANTHER; PTHR10788; TREHALOSE-6-PHOSPHATE SYNTHASE; 1.
DR Pfam; PF00982; Glyco_transf_20; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676,
KW ECO:0000256|RuleBase:RU362045};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU362045}.
SQ SEQUENCE 467 AA; 52853 MW; 696622AE57B58BB5 CRC64;
MSRLVVVSNR IADPRKAAAG GLAVAVKDSL QETGGVWFGW SGRLRGSDQP ARSDDVQIQN
VGGIQLATID LDPQDYDAYY LGYSNNVLWP VFHYRLDLAQ FDRRFADGYR RVNQLFARKL
RTLLRPDDTV WVHDYQLIPL AAELRAMGCT NPIGFFLHIP MPPPPIMAAI PEHEWLMRSL
FAYDLVGFQT EADLLHFEHY VESEAGAARL PDGRLRAFGR TLSAGAFPIG INVDEFAALA
DDRDGIDMFE RMRDEYSRRQ LLVGVDRLDY TKGLPQRVHA FRELLEQYPE NRNRATLIQI
AAPSREDLGA YDDLRREMDS LCGAINGDYG ELEWMPMRYI HRTVARKRLP GLYRASRVAL
VTPLRDGMNL VAKEFLAAQD EADPGVLVLS RFAGAAEQLK EALLVNPYDT QGTAQAIQRA
LAMPLEERRQ RHTALMATVR KTDVHWWRTR FLEALIEAAE VAATTEP
//
