ID PUR2_METMJ Reviewed; 430 AA.
AC A3CU83;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 01-MAY-2013, entry version 45.
DE RecName: Full=Phosphoribosylamine--glycine ligase;
DE EC=6.3.4.13;
DE AltName: Full=GARS;
DE AltName: Full=Glycinamide ribonucleotide synthetase;
DE AltName: Full=Phosphoribosylglycinamide synthetase;
GN Name=purD; OrderedLocusNames=Memar_1000;
OS Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1).
OC Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
OC Methanomicrobiaceae; Methanoculleus.
OX NCBI_TaxID=368407;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 35101 / DSM 1498 / JR1;
RX PubMed=21304656; DOI=10.4056/sigs.32535;
RA Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A.,
RA Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C.,
RA Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S.,
RA Hauser L., Land M., Lucas S., Richardson P., Whitman W.B.,
RA Kyrpides N.C.;
RT "Complete genome sequence of Methanoculleus marisnigri Romesser et al.
RT 1981 type strain JR1.";
RL Stand. Genomic Sci. 1:189-196(2009).
CC -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC similarity).
CC -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC ribose 1-diphosphate: step 2/2.
CC -!- SIMILARITY: Belongs to the GARS family.
CC -!- SIMILARITY: Contains 1 ATP-grasp domain.
CC -----------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution-NoDerivs License
CC -----------------------------------------------------------------------
DR EMBL; CP000562; ABN56933.1; -; Genomic_DNA.
DR RefSeq; YP_001046915.1; NC_009051.1.
DR ProteinModelPortal; A3CU83; -.
DR STRING; 368407.Memar_1000; -.
DR EnsemblBacteria; ABN56933; ABN56933; Memar_1000.
DR GeneID; 4847274; -.
DR KEGG; mem:Memar_1000; -.
DR eggNOG; COG0151; -.
DR HOGENOM; HOG000033464; -.
DR KO; K01945; -.
DR OMA; MGAYTPL; -.
DR ProtClustDB; PRK00885; -.
DR BioCyc; MMAR368407:GH7L-1053-MONOMER; -.
DR UniPathway; UPA00074; UER00125.
DR GO; GO:0005524; F:ATP binding; IEA:HAMAP.
DR GO; GO:0000287; F:magnesium ion binding; IEA:HAMAP.
DR GO; GO:0030145; F:manganese ion binding; IEA:HAMAP.
DR GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:HAMAP.
DR GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR Gene3D; 3.30.1490.20; -; 1.
DR Gene3D; 3.30.470.20; -; 1.
DR Gene3D; 3.40.50.20; -; 1.
DR Gene3D; 3.90.600.10; -; 1.
DR HAMAP; MF_00138; GARS; 1; -.
DR InterPro; IPR011761; ATP-grasp.
DR InterPro; IPR013815; ATP_grasp_subdomain_1.
DR InterPro; IPR013816; ATP_grasp_subdomain_2.
DR InterPro; IPR016185; PreATP-grasp_dom.
DR InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR InterPro; IPR000115; PRibGlycinamide_synth.
DR InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR InterPro; IPR020562; PRibGlycinamide_synth_N.
DR InterPro; IPR011054; Rudment_hybrid_motif.
DR Pfam; PF01071; GARS_A; 1.
DR Pfam; PF02843; GARS_C; 1.
DR Pfam; PF02844; GARS_N; 1.
DR SUPFAM; SSF52440; PreATP-grasp-like; 1.
DR SUPFAM; SSF51246; Rudmnt_hyb_motif; 1.
DR TIGRFAMs; TIGR00877; purD; 1.
DR PROSITE; PS50975; ATP_GRASP; 1.
DR PROSITE; PS00184; GARS; 1.
PE 3: Inferred from homology;
KW ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW Metal-binding; Nucleotide-binding; Purine biosynthesis.
FT CHAIN 1 430 Phosphoribosylamine--glycine ligase.
FT /FTId=PRO_1000018826.
FT DOMAIN 111 315 ATP-grasp.
FT NP_BIND 138 193 ATP (By similarity).
FT METAL 273 273 Magnesium or manganese 1 (By similarity).
FT METAL 285 285 Magnesium or manganese 1 (By similarity).
FT METAL 285 285 Magnesium or manganese 2 (By similarity).
FT METAL 287 287 Magnesium or manganese 2 (By similarity).
SQ SEQUENCE 430 AA; 46875 MW; EF618C6B8DAE8F24 CRC64;
MDMKVLVVGG GGREHAITRA LSCNSDTKIF SVMARKNPGI ARLSERVLLE KETNIEKVIP
FATNCGVDAA VIGPEAPLEA GIVDRLEAAG IPSLGPTRAA ARIETDKAFC RRLMERHGIA
GCPEYRVCRD PEEARRFIES YDGDLAVKPI GLTGGKGVRI MGEHVDVAGA VEYAREIGGS
VVLEERLVGE EFTLQAFVDG EHLIPMPLVQ DHKRAYEGDV GPNTGGMGSY SLADHMLPFV
SRRDYEKALR IMTDTVAAMR AEGTPYRGIL YGQFMNTREG PKVIEFNARF GDPEAMNVLS
LLESDFAGIV GHIIEGDLAP SHVRFAKKAT VCKYLVPEGY PDAPRANEPL TLGDYGDALL
YYASVEERDG TLHTLTSRTL AFVGRGETLE EAEAIAEKAA SSVSGSVFHR RDIGTPEILE
KRCQHMREIL
//
