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Database: UniProt
Entry: A3CU83
LinkDB: A3CU83
Original site: A3CU83 
ID   PUR2_METMJ              Reviewed;         430 AA.
AC   A3CU83;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAR-2007, sequence version 1.
DT   19-FEB-2014, entry version 48.
DE   RecName: Full=Phosphoribosylamine--glycine ligase;
DE            EC=6.3.4.13;
DE   AltName: Full=GARS;
DE   AltName: Full=Glycinamide ribonucleotide synthetase;
DE   AltName: Full=Phosphoribosylglycinamide synthetase;
GN   Name=purD; OrderedLocusNames=Memar_1000;
OS   Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1).
OC   Archaea; Euryarchaeota; Methanomicrobia; Methanomicrobiales;
OC   Methanomicrobiaceae; Methanoculleus.
OX   NCBI_TaxID=368407;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35101 / DSM 1498 / JR1;
RX   PubMed=21304656; DOI=10.4056/sigs.32535;
RA   Anderson I.J., Sieprawska-Lupa M., Lapidus A., Nolan M., Copeland A.,
RA   Glavina Del Rio T., Tice H., Dalin E., Barry K., Saunders E., Han C.,
RA   Brettin T., Detter J.C., Bruce D., Mikhailova N., Pitluck S.,
RA   Hauser L., Land M., Lucas S., Richardson P., Whitman W.B.,
RA   Kyrpides N.C.;
RT   "Complete genome sequence of Methanoculleus marisnigri Romesser et al.
RT   1981 type strain JR1.";
RL   Stand. Genomic Sci. 1:189-196(2009).
CC   -!- CATALYTIC ACTIVITY: ATP + 5-phospho-D-ribosylamine + glycine = ADP
CC       + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
CC   -!- COFACTOR: Binds 2 magnesium or manganese ions per subunit (By
CC       similarity).
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC       ribose 1-diphosphate: step 2/2.
CC   -!- SIMILARITY: Belongs to the GARS family.
CC   -!- SIMILARITY: Contains 1 ATP-grasp domain.
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DR   EMBL; CP000562; ABN56933.1; -; Genomic_DNA.
DR   RefSeq; YP_001046915.1; NC_009051.1.
DR   ProteinModelPortal; A3CU83; -.
DR   STRING; 368407.Memar_1000; -.
DR   EnsemblBacteria; ABN56933; ABN56933; Memar_1000.
DR   GeneID; 4847274; -.
DR   KEGG; mem:Memar_1000; -.
DR   eggNOG; COG0151; -.
DR   HOGENOM; HOG000033464; -.
DR   KO; K01945; -.
DR   OMA; DPEAMNV; -.
DR   ProtClustDB; PRK00885; -.
DR   BioCyc; MMAR368407:GH7L-1018-MONOMER; -.
DR   UniPathway; UPA00074; UER00125.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0030145; F:manganese ion binding; IEA:UniProtKB-HAMAP.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-HAMAP.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.30.470.20; -; 1.
DR   Gene3D; 3.40.50.20; -; 1.
DR   Gene3D; 3.90.600.10; -; 1.
DR   HAMAP; MF_00138; GARS; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR013816; ATP_grasp_subdomain_2.
DR   InterPro; IPR016185; PreATP-grasp_dom.
DR   InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR   InterPro; IPR000115; PRibGlycinamide_synth.
DR   InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR   InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR   InterPro; IPR020562; PRibGlycinamide_synth_N.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   Pfam; PF02844; GARS_N; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00184; GARS; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Ligase; Magnesium; Manganese;
KW   Metal-binding; Nucleotide-binding; Purine biosynthesis.
FT   CHAIN         1    430       Phosphoribosylamine--glycine ligase.
FT                                /FTId=PRO_1000018826.
FT   DOMAIN      111    315       ATP-grasp.
FT   NP_BIND     138    193       ATP (By similarity).
FT   METAL       273    273       Magnesium or manganese 1 (By similarity).
FT   METAL       285    285       Magnesium or manganese 1 (By similarity).
FT   METAL       285    285       Magnesium or manganese 2 (By similarity).
FT   METAL       287    287       Magnesium or manganese 2 (By similarity).
SQ   SEQUENCE   430 AA;  46875 MW;  EF618C6B8DAE8F24 CRC64;
     MDMKVLVVGG GGREHAITRA LSCNSDTKIF SVMARKNPGI ARLSERVLLE KETNIEKVIP
     FATNCGVDAA VIGPEAPLEA GIVDRLEAAG IPSLGPTRAA ARIETDKAFC RRLMERHGIA
     GCPEYRVCRD PEEARRFIES YDGDLAVKPI GLTGGKGVRI MGEHVDVAGA VEYAREIGGS
     VVLEERLVGE EFTLQAFVDG EHLIPMPLVQ DHKRAYEGDV GPNTGGMGSY SLADHMLPFV
     SRRDYEKALR IMTDTVAAMR AEGTPYRGIL YGQFMNTREG PKVIEFNARF GDPEAMNVLS
     LLESDFAGIV GHIIEGDLAP SHVRFAKKAT VCKYLVPEGY PDAPRANEPL TLGDYGDALL
     YYASVEERDG TLHTLTSRTL AFVGRGETLE EAEAIAEKAA SSVSGSVFHR RDIGTPEILE
     KRCQHMREIL
//
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