ID A4F5C7_SORCE Unreviewed; 5005 AA.
AC A4F5C7;
DT 17-APR-2007, integrated into UniProtKB/TrEMBL.
DT 17-APR-2007, sequence version 1.
DT 27-MAR-2024, entry version 81.
DE SubName: Full=Polyketide synthase {ECO:0000313|EMBL:CAL58685.1};
GN Name=spiH {ECO:0000313|EMBL:CAL58685.1};
OS Sorangium cellulosum (Polyangium cellulosum).
OC Bacteria; Myxococcota; Polyangia; Polyangiales; Polyangiaceae; Sorangium.
OX NCBI_TaxID=56 {ECO:0000313|EMBL:CAL58685.1};
RN [1] {ECO:0000313|EMBL:CAL58685.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=So ce90 {ECO:0000313|EMBL:CAL58685.1};
RX PubMed=17317575; DOI=10.1016/j.chembiol.2006.11.013;
RA Frank B., Knauber J., Steinmetz H., Scharfe M., Bloecker H., Beyer S.,
RA Mueller R.;
RT "Spiroketal polyketide formation in Sorangium: identification and analysis
RT of the biosynthetic gene cluster for the highly cytotoxic spirangienes.";
RL Chem. Biol. 14:221-233(2007).
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DR EMBL; AM407731; CAL58685.1; -; Genomic_DNA.
DR GO; GO:0004315; F:3-oxoacyl-[acyl-carrier-protein] synthase activity; IEA:InterPro.
DR GO; GO:0031177; F:phosphopantetheine binding; IEA:InterPro.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IEA:InterPro.
DR CDD; cd08952; KR_1_SDR_x; 2.
DR CDD; cd08956; KR_3_FAS_SDR_x; 1.
DR CDD; cd00833; PKS; 3.
DR Gene3D; 3.30.70.3290; -; 3.
DR Gene3D; 3.40.47.10; -; 3.
DR Gene3D; 6.10.140.1830; -; 1.
DR Gene3D; 1.10.1200.10; ACP-like; 3.
DR Gene3D; 3.40.366.10; Malonyl-Coenzyme A Acyl Carrier Protein, domain 2; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 3.
DR Gene3D; 3.10.129.110; Polyketide synthase dehydratase; 1.
DR InterPro; IPR001227; Ac_transferase_dom_sf.
DR InterPro; IPR036736; ACP-like_sf.
DR InterPro; IPR014043; Acyl_transferase.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR018201; Ketoacyl_synth_AS.
DR InterPro; IPR014031; Ketoacyl_synth_C.
DR InterPro; IPR014030; Ketoacyl_synth_N.
DR InterPro; IPR016036; Malonyl_transacylase_ACP-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR032821; PKS_assoc.
DR InterPro; IPR020841; PKS_Beta-ketoAc_synthase_dom.
DR InterPro; IPR041618; PKS_DE.
DR InterPro; IPR042104; PKS_dehydratase_sf.
DR InterPro; IPR020807; PKS_DH.
DR InterPro; IPR049551; PKS_DH_C.
DR InterPro; IPR049552; PKS_DH_N.
DR InterPro; IPR013968; PKS_KR.
DR InterPro; IPR020806; PKS_PP-bd.
DR InterPro; IPR009081; PP-bd_ACP.
DR InterPro; IPR006162; Ppantetheine_attach_site.
DR InterPro; IPR016039; Thiolase-like.
DR PANTHER; PTHR43775; FATTY ACID SYNTHASE; 1.
DR PANTHER; PTHR43775:SF51; PHENOLPHTHIOCEROL_PHTHIOCEROL POLYKETIDE SYNTHASE SUBUNIT E; 1.
DR Pfam; PF00698; Acyl_transf_1; 3.
DR Pfam; PF16197; KAsynt_C_assoc; 3.
DR Pfam; PF00109; ketoacyl-synt; 3.
DR Pfam; PF02801; Ketoacyl-synt_C; 3.
DR Pfam; PF08659; KR; 3.
DR Pfam; PF18369; PKS_DE; 2.
DR Pfam; PF21089; PKS_DH_N; 1.
DR Pfam; PF00550; PP-binding; 3.
DR Pfam; PF14765; PS-DH; 1.
DR SMART; SM00827; PKS_AT; 3.
DR SMART; SM00826; PKS_DH; 1.
DR SMART; SM00822; PKS_KR; 3.
DR SMART; SM00825; PKS_KS; 3.
DR SMART; SM00823; PKS_PP; 3.
DR SMART; SM01294; PKS_PP_betabranch; 3.
DR SUPFAM; SSF47336; ACP-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 3.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 6.
DR SUPFAM; SSF55048; Probable ACP-binding domain of malonyl-CoA ACP transacylase; 3.
DR SUPFAM; SSF53901; Thiolase-like; 3.
DR PROSITE; PS50075; CARRIER; 3.
DR PROSITE; PS00606; KS3_1; 3.
DR PROSITE; PS52004; KS3_2; 3.
DR PROSITE; PS00012; PHOSPHOPANTETHEINE; 3.
PE 4: Predicted;
KW Phosphopantetheine {ECO:0000256|ARBA:ARBA00022450};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 23..449
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 1476..1554
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 1576..2002
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 3037..3115
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
FT DOMAIN 3139..3565
FT /note="Ketosynthase family 3 (KS3)"
FT /evidence="ECO:0000259|PROSITE:PS52004"
FT DOMAIN 4847..4922
FT /note="Carrier"
FT /evidence="ECO:0000259|PROSITE:PS50075"
SQ SEQUENCE 5005 AA; 524233 MW; DB2CA6C11726FD1A CRC64;
MRRLTQELHR SETRLRATEE RRHEPIAIVG LGLRLPGGIH DRDTLWTFLE EGRDAIAPIP
ASRWNADATY DPDPDAVGKS YVRDAAMLDR VDLFDADFFG ISPREAKYVD PQHRLLLETS
WQALEDAGIV PASLRDSKTG VFVGTGASDY AFLQSDRDAS EAYAFMGMIS SFAAGRLAFT
LGLQGPALSI DTACSSSLVA LHLACQSLRQ GECDLALVAG VQVMSSPEVF VLLSRTRALA
SDGRSKTFSA NADGYGRGEG VVVLAVERLR DARAKGRPIL AVIRGSAVNH DGTSSGITVP
NGPAQQKVLR AALDDARLVP ADVDVVECHG TGTSIGDPIE VNALAAVYGE GRPKDRPLFL
GALKTNIGHL EFASGLAGVA KMVASMRHAT LPATLHTSPL NPLVDWDALP VRVVDAARPW
TRRDDGAPRR AGVTAIGLSG TNAHVIVEEA PAEPEPTTPD AAPALPAVPV LLSGKTDEAL
RAQAARLHAH LAGRPDARLV DIAASLATTR THFDRRAAVV AADRDELLGA LDALARGEAG
PGSVVASAIP AGRVVFVFPG QGSQWVGMAR ALLASSVVFR DEIAACERAL APHVAWSLGA
VLRGDGDEAT LLGRVDVVQP VLFAVMVALA ALWRSIGVTP DAVVGHSQGE IAAAYVAGAL
SLEDAAKVVA LRARALTKIA GRGAMAAVEL GARDTEARLA PFGDAIAIAA INSPRATLVA
GDTDAIDALV RDLEAAQIFA RKVRVDYASH SAHVEAIERE LLADLAGIEP RAGAVPLYSA
VTGAKLDGSR LDAAHWFRNL RSTVRFEDAT RALHDDGHRF FVEVSPHPVL ALALDETLSS
IGGDAAVVSS LRRDEGDLER FLLSAGELHA RGHAIDWGAF FAPLGARRVT LPTYAFQRER
FWLDAGDASD DEDAAPGASA EETAFWRAIE RGDVAALSDA LHVADSGRRS ALESLMPALS
AWRRSRREQS TVGAWRYRVE WRPVSAASRG DVAGTWLVVA PAGVASDLAS ALAAALTERG
ADVVPLALEA SDITAHDAAS RLRDAIAGRP AIRGVLTLTP LDTAPLDGRP AVPAGLARSL
ALVQALAELA IEAPIWIVTR GAVAIGRSDR IESPVQAMAW GLGRVVSLEQ PERWGGLVDL
PRAHADGALD ATAIERLVAA LGSREGEDEL ALRPTGLFAR RLARAPLGDA PAQRFEPRGT
VLVTGGTGAL GAHVARWLAR HGAAHLVLAS RRGAAAPGAE ALRAELTELG ARVTIAACDV
ADRAAIEELF AALPAEEPLT AIFHTAGVID DGVLGSLTPE RLETVLHGKA DAARTLHELT
KTRDLAAFVL FSSAAGVFGN AGQASYAAAN AFLDALAEER RALGLRATSV AWGAWAGAGM
LTFDVAQGLR RGGFAAMEPE IAVAALASAL DRDETHVTIA SIDWARFAPS YASARQRPLV
ADLPDARRAL DAQPSASTEH DETALVATLR ALSGEARLRH LVSLVLAQTA AVLGHADRSR
LDPQRGFFDL GLDSLMAVEL RRRLQKATGV KLPATITFDR PSPHRLAGFL LDALPLGGAA
PSTDARPRAA RADASDEPIA IVGVGLRLPG GVDDVDALFR LLEGERDAVA PIPASRWDVG
AVFDPDPEAK GKTYVRHAAM LDRVDLFDAG FFGISPREAR HVDPQHRLLL ETAWQALEAA
TIVPASLRDS TTGVFVGAGA SDYAVLQSSA EDAEAYAAMG TAASFAAGRL AFTLGLQGPA
LSIDTACSSS LVALHLACQS LRQGECDLAL AAGVQVMASP EVFVILSRTR ALAPDGRSKT
FSANADGFGR GEGVVVLALE RLRDARANGH PVLAVVRGTA VNHDGASSGI TAPNGSSQQK
VLRAALDDAR LAPTDVDVVE CHGTGTSLGD PIEVQALAAV YGEGRAPERP LRLGAVKTNI
GHLEFASGLA GVVKIVASLR HGTLPATIHT NPRNPHIDWD ALPVRVVDAA EPWERSADGS
PRRAGVSAFG LSGTNAHVIV EEAPEPDAPK AARGALPLSG ALPFVLSAKS DAALRAQAAA
LRDQLARTPD AALVDVAAAL ATTRSQFDHR AAIAASDHGA LVAALEGLAA GVAAPGTFVA
KGAADKLAFL FTGQGAQRAA MGRGLYDAFP VFRDALDAVA SHLDRELDRP LRDVLFAPKG
SELASLLDRT EFTQPALFAL EVALFRLVEA WGVTPDVLLG HSVGELAAAH VAGVLSLADA
CTLVAARARL MQALPARDGA MVTVHATEAE VLAALEGRDG RAEIAAINAP SSTVIAGDVD
AVLRVAAHFE ARGRKATRLR VSHAFHSHHM DPMLDAFRRV AEGLTFHPPR IPIVSNVTGR
LAADEIRSPD YWVRHVRSAV RFADGINTLE ADGVSSFFEL GPHGVLSALG ESALANAQRE
VAFVPALRDG RADVDALTAA LSSLHVRGHR VDWAAFFGPF DPQTVALPTY AFQRDRFWLD
APAGATRDEA APAAPEDAAF WRAVDAGDVG ALGATLNASG EDHLSALATL LPALSAWRRA
RDERSLVDAL RYRVVWKPLT PTAARDVAGT WLLVTLAGAE DALARELERA LTARGAEVIT
MPIAPAEADR VRLAVRVQGA LADAGELRGI VSFAALDETT LATHTALPAG LALTLRLVQA
LGDMGIEAPL WLVTRGAVST GRSDRLASAA QSMTWGLGRV VGLEHPERWG GLVDVADGDT
IDARALDRLV TLLASRDGED QLALRPTGIF ARRLVRAPLG DAPPARTYTA RGTSLVTGGT
GALGAHVARF LAGRGAEHLV LVSRRGEDAP GAGALRAELE ALGARVTLAA CDVADRASVA
ALLARLDAQG DPVRAVVHAG GVVAQAALAS TDLDGVQAVV AAKVRGALHL HELLGDRELD
AFVLFASGAG VWGSGQQGAY AAGNAFLDAL AEVRRAAGLT ATSIAWGAWA GGGMLADHAD
ADAEVELRKR GLVPMAPALA IAALAGALDH GETAVTVASV DWARFAPAFA SARPRPLLLD
LPEAQRAIEA PSAPAGADGA SADRALVEAL RPLSEADRAR HLVELVVAET AAVLGHADAS
AIDPHKGFFD LGLDSLVAVE LLRKLRARTG VELPATVTFD HPSPHRLATY VREALPLDAP
LAPADATTAR SSSAHAAIDE PIAIVGLGLR LPGGVEDREG LWSFLEQGRD GVGPIPKDRW
DADAIYDPDP EAKGKSYVRE AALLDRVDLF DAAFFGISPR EAKQVDPQHR LLLETAWQAL
EEATIVPASL KDTSTGVFVG IGLSDYALMQ GSAEETDAYA AMGTLSSFAA GRLAFTLGLQ
GPALSIDTAC SSSLVALHLA CQSLRKGECD LALAGGVQVM TLAEPFVLLS RTRALAADGR
SKTFSAHADG YGRGEGVVVL ALERLRDAEA RGRRVLAVVR GTAVNHDGAS SGITAPNGTS
QQKVLRAALG DAGLAPADVD VVECHGTGTA LGDPIEVQAL AAVYGEGRAA ERPLLLGAVK
SNIGHLEAAS GLAGVAKIVT ALEHGALPAT IHSRPRNPHV DWDALHVRVV DAAELWARRD
EETPRRAGVS AFGLSGTNAH AIIEEAPRAR RGAAATDEPP PAVPVVLSAK SERALRAQAE
RLRAHVTGRP GDALVDVAAS LATTRSHFEH RAAIVARDLG ALEDALAGLA EGRSPAGTVV
SRTTGGKLAL LFTGQGGQRA AMGRGLYGAF SVFRDAFDAA CSYLDRELEH PLRDVVFAPR
GSELAALLDE TLYTQTSLFA LEVALFRLVE AWGVTPHVLL GHSIGELVAA HVAGVLSLED
ACTLVAARAR LMHALPRRDA AMVAVHACES EVLDALDAMA GLDGRASIAA VNAPSSTVVA
GDREAVLAVA ARFEALGRKT SRLRVSHAFH SHHLDPMLEA FRRVAEGVRF EPPRIPIVSN
VTGARASNDD LCSADYWVRH ARGTVRFADG LEALRADGVS TFLELGPHAV LCALGHETLE
SEAVAFLPAL RDGRDDVDTL TAALGALHAR GHHVDWDAFF APFNAGRVAL PTYAFQRERF
WLDMPKAKSS RAAAADSAGR YPLAGTRLDL PDGSAVHTLD IGPGAQPYLA DHSVYGRIVV
PGAFHLAVLL AVAESHWPDS PLELRDVEFV RALTFEEPSE TVAVRVHLTP AGGDGKGFHA
TVATQTEGAW TTHATATLGA APPSALARHP RAAHEPLAGD EPSRAVARLM ASFEAFNVEW
GPKWRWLRQT THAGDRTGVG YLAAPDGVPT DDAPLPGGLV DNAFAVVGSA VTGLPAHDNR
STSNVPPLPF SIERLVWYGR AETPCWADFV MRGEITPDAD RYIGDITLWS REGASSEGAS
REGTPIATIE GLAFRRAPAD KFLPELPARN LYALTWPEQP AASRPPRGRW ALLGEDSLGI
ATGIAADAYP DVDALRDALG RGAPCPDTVA VCLATAHGPS DTDETELVSS VHTAAARALA
LLNAWLSDER LASSTLVLLT QRAVATCAGE DVLDLASAAL WGLVRSAQAE NRDRAILLVD
LDGSDASRRA LPYAVGGALD DAEPQLALRD GRRLAPRLAR VAAPTAATAN AATSTSATPR
ALDPEGTVLV TGGTGTLGAL VARRLVERHG VKHLVLLSRR GPDAPGATDL AAELQARGAS
VVLAAADAAE PAALARVLLA IPHDRPLTAV VHAAGTLDDG VLSSMTPARL SAVLRAKVDA
AVNLDEQTRH SPLRAFVLFS SLSGVLGSPA QSNYAAANAF LDALAHRRAA RGLPAVALDW
GYWAQTSGLT KHLEQADLRR IARGGLRPLA SDEALALFDL ALAQDAPCLV PAAFDADALR
KSGDALSALL RGLVAGPRAR AQRASGAGAA SALKLRLASL APLERHRMLL DVVRADVATV
LGVASPLDIE PGRPLQELGL DSLMAIELRN RLAAATGLRL SATLLFDHPT PEALAKLISG
KLLHDEAAPP STSAAEIDRL EGALASAYAN EAIRDELTVR MKAILSKWMA AREPSPDDGL
ASRLDTANDD ELFRMIDHVR AEAAT
//
