ID A4L724_CLOBU Unreviewed; 574 AA.
AC A4L724;
DT 01-MAY-2007, integrated into UniProtKB/TrEMBL.
DT 05-FEB-2008, sequence version 2.
DT 27-MAR-2024, entry version 72.
DE SubName: Full=2Fe-2S iron-sulfur cluster binding domain-containing protein {ECO:0000313|EMBL:NAS16916.1};
DE SubName: Full=4Fe-4S dicluster domain-containing protein {ECO:0000313|EMBL:RQN07617.1};
DE SubName: Full=Ferredoxin {ECO:0000313|EMBL:GEQ20136.1};
DE SubName: Full=Hydrogenase {ECO:0000313|EMBL:ABO42543.2};
DE EC=1.12.7.2 {ECO:0000313|EMBL:ABO42543.2};
GN Name=hydA {ECO:0000313|EMBL:ABO42543.2};
GN ORFNames=CBU02nite_06420 {ECO:0000313|EMBL:GEQ20136.1}, EHW71_15410
GN {ECO:0000313|EMBL:RQN07617.1}, GND98_003205
GN {ECO:0000313|EMBL:NAS16916.1};
OS Clostridium butyricum.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Clostridium.
OX NCBI_TaxID=1492 {ECO:0000313|EMBL:ABO42543.2};
RN [1] {ECO:0000313|EMBL:ABO42543.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CGS5 {ECO:0000313|EMBL:ABO42543.2};
RX PubMed=17909787; DOI=10.1007/s00253-007-1196-1;
RA Wang M.Y., Olson B.H., Chang J.S.;
RT "Improving PCR and qPCR detection of hydrogenase A (hydA) associated with
RT Clostridia in pure cultures and environmental sludges using bovine serum
RT albumin.";
RL Appl. Microbiol. Biotechnol. 77:645-656(2007).
RN [2] {ECO:0000313|EMBL:ABO42543.2}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=CGS5 {ECO:0000313|EMBL:ABO42543.2};
RA Chang J.-S., Wang M.-Y., Olson B.H.;
RT "Development of the hydrogenase sequence of indigenous Clostridium
RT butyricum CGS5.";
RL Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:RQN07617.1, ECO:0000313|Proteomes:UP000269424}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JKT37 {ECO:0000313|EMBL:RQN07617.1,
RC ECO:0000313|Proteomes:UP000269424};
RA Roslan R., Abdul P.M., Md Jahim J.;
RT "Whole genome sequence of Clostridium butyricum JKT37, isolated from palm
RT oil mill effluent (POME).";
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:GEQ20136.1, ECO:0000313|Proteomes:UP000321089}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 3858 {ECO:0000313|EMBL:GEQ20136.1,
RC ECO:0000313|Proteomes:UP000321089};
RA Hosoyama A., Uohara A., Ohji S., Ichikawa N.;
RT "Whole genome shotgun sequence of Clostridium butyricum NBRC 3858.";
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000313|EMBL:NAS16916.1, ECO:0000313|Proteomes:UP000474042}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=S3 {ECO:0000313|EMBL:NAS16916.1,
RC ECO:0000313|Proteomes:UP000474042};
RA Zhou J.;
RT "Genome sequence of a 1,3-propanediol producer, Clostridium butyricum S3.";
RL Submitted (JAN-2020) to the EMBL/GenBank/DDBJ databases.
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DR EMBL; EF450251; ABO42543.2; -; Genomic_DNA.
DR EMBL; BKBC01000006; GEQ20136.1; -; Genomic_DNA.
DR EMBL; WOFV02000005; NAS16916.1; -; Genomic_DNA.
DR EMBL; RQKF01000047; RQN07617.1; -; Genomic_DNA.
DR RefSeq; WP_046057752.1; NZ_WOFV02000005.1.
DR Proteomes; UP000269424; Unassembled WGS sequence.
DR Proteomes; UP000321089; Unassembled WGS sequence.
DR Proteomes; UP000474042; Unassembled WGS sequence.
DR GO; GO:0008901; F:ferredoxin hydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR CDD; cd00207; fer2; 1.
DR Gene3D; 3.10.20.740; -; 1.
DR Gene3D; 3.30.70.20; -; 1.
DR Gene3D; 3.40.50.1780; -; 1.
DR Gene3D; 4.10.260.20; Iron hydrogenase, small subunit; 1.
DR InterPro; IPR036010; 2Fe-2S_ferredoxin-like_sf.
DR InterPro; IPR001041; 2Fe-2S_ferredoxin-type.
DR InterPro; IPR017896; 4Fe4S_Fe-S-bd.
DR InterPro; IPR017900; 4Fe4S_Fe_S_CS.
DR InterPro; IPR009016; Fe_hydrogenase.
DR InterPro; IPR004108; Fe_hydrogenase_lsu_C.
DR InterPro; IPR003149; Fe_hydrogenase_ssu.
DR InterPro; IPR036991; Fe_hydrogenase_ssu_sf.
DR InterPro; IPR013352; Fe_hydrogenase_subset.
DR NCBIfam; TIGR02512; FeFe_hydrog_A; 1.
DR NCBIfam; NF040762; Hydr_FeFe_Clost; 1.
DR PANTHER; PTHR11615:SF6; NADH-UBIQUINONE OXIDOREDUCTASE 75 KDA SUBUNIT, MITOCHONDRIAL; 1.
DR PANTHER; PTHR11615; NITRATE, FORMATE, IRON DEHYDROGENASE; 1.
DR Pfam; PF02906; Fe_hyd_lg_C; 1.
DR Pfam; PF02256; Fe_hyd_SSU; 1.
DR Pfam; PF13510; Fer2_4; 1.
DR Pfam; PF12838; Fer4_7; 1.
DR SMART; SM00902; Fe_hyd_SSU; 1.
DR SUPFAM; SSF54292; 2Fe-2S ferredoxin-like; 1.
DR SUPFAM; SSF54862; 4Fe-4S ferredoxins; 1.
DR SUPFAM; SSF53920; Fe-only hydrogenase; 1.
DR PROSITE; PS51085; 2FE2S_FER_2; 1.
DR PROSITE; PS00198; 4FE4S_FER_1; 1.
DR PROSITE; PS51379; 4FE4S_FER_2; 2.
PE 4: Predicted;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Oxidoreductase {ECO:0000313|EMBL:ABO42543.2}.
FT DOMAIN 1..78
FT /note="2Fe-2S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51085"
FT DOMAIN 138..167
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51379"
FT DOMAIN 181..210
FT /note="4Fe-4S ferredoxin-type"
FT /evidence="ECO:0000259|PROSITE:PS51379"
SQ SEQUENCE 574 AA; 62983 MW; 9E2906AF5155CDB0 CRC64;
MINLIIDEKN VQVPEGTSII QAALDNGIDI PSLCYLKECS NVGKCGVCAV EVEGKKVLSL
ACLTKVEEGM VVRTNTEATQ ERVKMRVAAL LDKHEFKCGP CPRRETCEFL KLVIKTKAKA
NKPFLPTDKT EFLDDRSKSI TIDRSKCVLC GRCVAACKEK TGTGNIEIAG KGPDRKVQAI
EGKCFDETNC LLCGQCVAAC PVAALNEKPH IDRVKEALED PNKHVIVAMA PAVRTSMGEL
FKMGYGVDVT GKLYSSLRQL GFDKVFDINF GADMTIMEEA TEFIERINNN GPFPMFTSCC
PAWVRQAENY YPELLGNLSS AKSPQQIFGA ASKTYYPTVE GLDPKSVYTV TIMPCTAKKY
EADRTEMENE GLRNIDAVLT TRELAKMIKD AKINFAALED EKADPAMGEY TGAGVIFGAT
GGVMEAALRS AKDFVEDKDL TDIEYKQVRG LDGIKEATVE IGGKNYNVAV INGSANLTKF
VEGGQMDEKQ YHFVEVMSCP GGCVNGGGQP HVAAKDRDTA NVRAVRASVL YNQDKTAVKR
KSHKNEALMK MYNEYMGMPG QHKAHELLHL KYTK
//
