ID A5Z3E6_9FIRM Unreviewed; 1746 AA.
AC A5Z3E6;
DT 24-JUL-2007, integrated into UniProtKB/TrEMBL.
DT 24-JUL-2007, sequence version 1.
DT 27-MAR-2024, entry version 70.
DE RecName: Full=glucan endo-1,3-beta-D-glucosidase {ECO:0000256|ARBA:ARBA00012780};
DE EC=3.2.1.39 {ECO:0000256|ARBA:ARBA00012780};
GN ORFNames=EUBVEN_00192 {ECO:0000313|EMBL:EDM52541.1};
OS Eubacterium ventriosum ATCC 27560.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Eubacteriaceae;
OC Eubacterium.
OX NCBI_TaxID=411463 {ECO:0000313|EMBL:EDM52541.1, ECO:0000313|Proteomes:UP000006000};
RN [1] {ECO:0000313|EMBL:EDM52541.1, ECO:0000313|Proteomes:UP000006000}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27560 {ECO:0000313|EMBL:EDM52541.1,
RC ECO:0000313|Proteomes:UP000006000};
RA Fulton L., Clifton S., Fulton B., Xu J., Minx P., Pepin K.H., Johnson M.,
RA Thiruvilangam P., Bhonagiri V., Nash W.E., Mardis E.R., Wilson R.K.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:EDM52541.1, ECO:0000313|Proteomes:UP000006000}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27560 {ECO:0000313|EMBL:EDM52541.1,
RC ECO:0000313|Proteomes:UP000006000};
RA Sudarsanam P., Ley R., Guruge J., Turnbaugh P.J., Mahowald M., Liep D.,
RA Gordon J.;
RT "Draft genome sequence of Eubacterium ventriosum (ATCC 27560).";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC beta-D-glucans.; EC=3.2.1.39;
CC Evidence={ECO:0000256|ARBA:ARBA00000382};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 81 family.
CC {ECO:0000256|ARBA:ARBA00010730}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EDM52541.1}.
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DR EMBL; AAVL02000022; EDM52541.1; -; Genomic_DNA.
DR STRING; 411463.EUBVEN_00192; -.
DR CAZy; CBM32; Carbohydrate-Binding Module Family 32.
DR CAZy; CBM6; Carbohydrate-Binding Module Family 6.
DR CAZy; GH81; Glycoside Hydrolase Family 81.
DR eggNOG; COG3325; Bacteria.
DR eggNOG; COG5498; Bacteria.
DR HOGENOM; CLU_003268_0_0_9; -.
DR OrthoDB; 9760892at2; -.
DR Proteomes; UP000006000; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProt.
DR GO; GO:0052861; F:glucan endo-1,3-beta-glucanase activity, C-3 substituted reducing group; IEA:InterPro.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:UniProtKB-KW.
DR CDD; cd04080; CBM6_cellulase-like; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 4.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006584; Cellulose-bd_IV.
DR InterPro; IPR005084; CMB_fam6.
DR InterPro; IPR005200; Endo-beta-glucanase.
DR InterPro; IPR000421; FA58C.
DR InterPro; IPR036116; FN3_sf.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR040720; GH81_C.
DR InterPro; IPR013783; Ig-like_fold.
DR PANTHER; PTHR31983; ENDO-1,3(4)-BETA-GLUCANASE 1; 1.
DR PANTHER; PTHR31983:SF0; ENDO-1,3(4)-BETA-GLUCANASE 2; 1.
DR Pfam; PF00754; F5_F8_type_C; 3.
DR Pfam; PF17652; Glyco_hydro81C; 1.
DR SMART; SM00606; CBD_IV; 1.
DR SUPFAM; SSF49265; Fibronectin type III; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 4.
DR PROSITE; PS51175; CBM6; 1.
DR PROSITE; PS50022; FA58C_3; 3.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Cell wall biogenesis/degradation {ECO:0000256|ARBA:ARBA00023316};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Polysaccharide degradation {ECO:0000256|ARBA:ARBA00023326};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 17..35
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 56..73
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 75..249
FT /note="F5/8 type C"
FT /evidence="ECO:0000259|PROSITE:PS50022"
FT DOMAIN 254..400
FT /note="F5/8 type C"
FT /evidence="ECO:0000259|PROSITE:PS50022"
FT DOMAIN 1270..1391
FT /note="CBM6"
FT /evidence="ECO:0000259|PROSITE:PS51175"
FT DOMAIN 1411..1554
FT /note="F5/8 type C"
FT /evidence="ECO:0000259|PROSITE:PS50022"
FT REGION 1564..1637
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1746 AA; 196026 MW; 8B30CF93CAF4CC1B CRC64;
MFFLSKIHKF KKVNLNLWIY VFWCAMFQIE NVSITKDGTG NKERRRVYMR RKFKRFLALT
LSAALCVTGI NYTKVNTQAA DRSAPWLLSD GRPAYASSTN GGNLPTYATD EKIGTQWEAV
ANKADQWLDI DLGGKADVSK VVINWQNSAS YGVAYQILVS DDEINWTKIY ETTKGNGGSE
KQVLKDDGTV DYTYYQDTLS SDSTDDYKLT EKNGRYVRVL INYSKSQSES ADKKSGWGAA
IRELEVYGIG DNKCNEPKSD APNIALGKKV EATSYSKPWW ASTPLAGSNA VDGNYDTYWL
SEGEDNVQSK CNQSLTVDLG KKYTLGRVFL QWQVEYGNIW DLQVSEDGTN FKTVYRQLQG
NGEDEDIKFY AENVRYVRMQ GILMGRGSGY SIRELQVFEY QQGDEKVNNT IPEIPKKKVV
TLGKGSYVID DANLLQPREP KYVSSKISTP IPSNDWWTSI VYTQYSDAMP ALPMSYKYSS
TGLSMYYADG LYNRADNGGM GVDSKYYDLT LNSSDITGKA SAKLDGYGDW SVNVKYSDDD
TEKMESTLIK GSPFAYTTFA NRDSVELNVN NLVKFLDKNG SEIITEDGKE VTIDHFLVET
ENESEAPGDG NKKQYHYYGI YLPENSKATK VGSKLKIKLG NNDNYVVIGA LAVEEPIKLL
ASQSEVDSIS KPSLLRDGDA VKQLEYMYKY AYSYVTDTVV DYSYDDAKSI CTTTYKVSVS
QKRTGSGISN STLLCMMPHQ WKYSDATYET TNGEALIYTS SRGDLRIHEG NEFSYSQKFN
GIIPQYTTPS ESNGYDKEWM YAYLKQFTDS ALKSYWVADP YWEGKKSHPI AMGILIADQL
GEYETRDKLI DVLKKIMANW LTYDGDEDYP YYMYYHTSWG AVSGDGGDHG MAINLSDHHF
LWAYFIFPAA VLASYDSTFV DDYGEMIELL IRDCMNPDKN DDMLPFMRNF DVYEGHSWAG
GYGDNNSGNN QESASEATFG WAGLYLWGLV TGNKKYEKAG IWGYTSEVNA IEQYWFNVDK
GTDRDSHNWA SDYGTNVYGD SNVNNIPYIG MIWGLGYTNG TYFSGNPCCI MGIHLLPVSP
AITYMGYNKE TAGKIWNDYE KVQEAYQKKL QANGEIDPEG WYHILWPFMA LSDAEGAASC
WANEYKSHLN ENGDYVGGVL STDEMFNSYW FIQNMCAKGN ICTDVWASNY TSYQVFAKTE
SGKTTYSAQV WNPGNDTITV NFVNKSGNLG SAKIPAHSLV SVDPTKNEDK TKDTTYVPHE
SRDKVCTVPG VVEAENYDTN FGCEPTTGDS EGGYVGWIDN GDSLIYTLDV KEEGDYIVDY
RVQCTDTNKN SAIKLKTDND SDYVLTTLLD NSTTEWKNVK TANTVHLKQG QYQMKLLMVD
GGFNLNYIKI YKAGTKPPTA ESDDLTLADL SGYPEIDLSG AKVIDVSSET GDNTGDKIID
GNFDSRWESK SEDPQYFTIE LGRNEEIGGI KIYWEAAAAK NYTIETSIDN ENWTNVFTRV
NGNGGQKNGD GNKSSGLESV SFDKVTTAKY VRIYCSERLT GYGDSVYEIK LYGRGKDQVD
VPETTTPVVT TKPNVTTPEN VTTNNEKTTT KPEQVTTKPE KVTTSNEQTT TTPVTKPQVT
SGNNKSDNKE TTVRGTVSAP SDDVNFVAGI GRTVIKKVKR TGNKKAKISL KKISGITNYQ
AKYSTKKSFK KKYTKTVNVK KINFTIKKLK ANNKYYIKVR AYMVKDGNRY YGKWSKKKTI
KKYKKK
//
