ID WDR35_RAT Reviewed; 1170 AA.
AC A6N6J5;
DT 08-MAR-2011, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 1.
DT 27-MAR-2024, entry version 109.
DE RecName: Full=WD repeat-containing protein 35;
DE AltName: Full=Naofen;
GN Name=Wdr35;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=Wistar;
RX PubMed=20193664; DOI=10.1016/j.bbrc.2010.02.133;
RA Feng G.G., Li C., Huang L., Tsunekawa K., Sato Y., Fujiwara Y., Komatsu T.,
RA Honda T., Fan J.H., Goto H., Koide T., Hasegawa T., Ishikawa N.;
RT "Naofen, a novel WD40-repeat protein, mediates spontaneous and tumor
RT necrosis factor-induced apoptosis.";
RL Biochem. Biophys. Res. Commun. 394:153-157(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: As a component of the IFT complex A (IFT-A), a complex
CC required for retrograde ciliary transport and entry into cilia of G
CC protein-coupled receptors (GPCRs), it is involved in ciliogenesis and
CC ciliary protein trafficking (By similarity). May promote CASP3
CC activation and TNF-stimulated apoptosis (PubMed:20193664).
CC {ECO:0000250|UniProtKB:Q9P2L0, ECO:0000269|PubMed:20193664}.
CC -!- SUBUNIT: Component of the IFT complex A (IFT-A) complex. IFT-A complex
CC is divided into a core subcomplex composed of IFT122:IFT140:WDR19 which
CC is associated with TULP3 and a peripheral subcomplex composed of
CC IFT43:WDR35:TTC21B. Interacts directy with IFT122, ITF43 and TTC21B.
CC Interacts with IFT43. Interacts with CFAP61 (By similarity).
CC {ECO:0000250|UniProtKB:Q8BND3, ECO:0000250|UniProtKB:Q9P2L0}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q8BND3}. Cytoplasm,
CC cytoskeleton, cilium axoneme {ECO:0000250|UniProtKB:Q8BND3}. Cytoplasm,
CC cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q8BND3}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in testis and at lower
CC levels in the brain (at protein level). Also present in other tissues,
CC including heart, uterus, spinal cord, ovary, liver, kidney, lung,
CC pancreas and stomach. {ECO:0000269|PubMed:20193664}.
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DR EMBL; EF613262; ABR22620.1; -; mRNA.
DR EMBL; CH473947; EDM03084.1; -; Genomic_DNA.
DR RefSeq; NP_001092810.1; NM_001099340.1.
DR AlphaFoldDB; A6N6J5; -.
DR SMR; A6N6J5; -.
DR STRING; 10116.ENSRNOP00000042019; -.
DR PhosphoSitePlus; A6N6J5; -.
DR PaxDb; 10116-ENSRNOP00000042019; -.
DR PeptideAtlas; A6N6J5; -.
DR Ensembl; ENSRNOT00000051970.5; ENSRNOP00000042019.4; ENSRNOG00000028783.5.
DR Ensembl; ENSRNOT00055009287; ENSRNOP00055007127; ENSRNOG00055005753.
DR Ensembl; ENSRNOT00060006028; ENSRNOP00060004447; ENSRNOG00060003647.
DR Ensembl; ENSRNOT00065007945; ENSRNOP00065005535; ENSRNOG00065005377.
DR GeneID; 503018; -.
DR KEGG; rno:503018; -.
DR AGR; RGD:1564116; -.
DR CTD; 57539; -.
DR RGD; 1564116; Wdr35.
DR eggNOG; KOG2041; Eukaryota.
DR GeneTree; ENSGT00940000155745; -.
DR HOGENOM; CLU_004048_1_0_1; -.
DR InParanoid; A6N6J5; -.
DR OrthoDB; 148480at2759; -.
DR PhylomeDB; A6N6J5; -.
DR Reactome; R-RNO-5610787; Hedgehog 'off' state.
DR Reactome; R-RNO-5620924; Intraflagellar transport.
DR PRO; PR:A6N6J5; -.
DR Proteomes; UP000002494; Chromosome 6.
DR Proteomes; UP000234681; Chromosome 6.
DR Bgee; ENSRNOG00000028783; Expressed in testis and 18 other cell types or tissues.
DR Genevisible; A6N6J5; RN.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; ISS:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; ISS:UniProtKB.
DR GO; GO:0030991; C:intraciliary transport particle A; ISS:UniProtKB.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
DR GO; GO:1990830; P:cellular response to leukemia inhibitory factor; ISO:RGD.
DR GO; GO:0071356; P:cellular response to tumor necrosis factor; IMP:RGD.
DR GO; GO:0071466; P:cellular response to xenobiotic stimulus; IEP:RGD.
DR GO; GO:0060271; P:cilium assembly; ISS:UniProtKB.
DR GO; GO:0035721; P:intraciliary retrograde transport; ISO:RGD.
DR GO; GO:0042073; P:intraciliary transport; ISO:RGD.
DR GO; GO:0097421; P:liver regeneration; IEP:RGD.
DR GO; GO:0010629; P:negative regulation of gene expression; IDA:RGD.
DR GO; GO:0045019; P:negative regulation of nitric oxide biosynthetic process; IDA:RGD.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IMP:RGD.
DR GO; GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IMP:RGD.
DR GO; GO:0045907; P:positive regulation of vasoconstriction; IDA:RGD.
DR GO; GO:0061512; P:protein localization to cilium; ISO:RGD.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEP:RGD.
DR GO; GO:0009636; P:response to toxic substance; IEP:RGD.
DR Gene3D; 1.25.40.470; -; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 1.
DR InterPro; IPR024977; Apc4-like_WD40_dom.
DR InterPro; IPR039857; Ift122/121.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR InterPro; IPR017233; WDR35.
DR PANTHER; PTHR12764:SF5; LD29485P; 1.
DR PANTHER; PTHR12764; WD REPEAT DOMAIN-RELATED; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF00400; WD40; 1.
DR PIRSF; PIRSF037536; WD_repeat_p35; 1.
DR SMART; SM00320; WD40; 4.
DR SUPFAM; SSF82171; DPP6 N-terminal domain-like; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cell projection; Cilium; Cilium biogenesis/degradation; Cytoplasm;
KW Cytoskeleton; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..1170
FT /note="WD repeat-containing protein 35"
FT /id="PRO_0000405406"
FT REPEAT 12..51
FT /note="WD 1"
FT REPEAT 69..108
FT /note="WD 2"
FT REPEAT 113..152
FT /note="WD 3"
FT REPEAT 154..193
FT /note="WD 4"
FT REPEAT 491..528
FT /note="WD 5"
SQ SEQUENCE 1170 AA; 132779 MW; 70792ECD36111DEF CRC64;
MFFYLSKKIA VPNNVKLKCI SWNKDQGFIA CGGEDGLLKV LRLETQTDDS KLRGLAAPSN
LSMNQNLEGH SGAVQVVTWN EQYQKLTTSD QNGLIIVWML YKGSWYEEMI NNRNKSVVRS
MSWNADGQKI CIVYEDGAVI VGSVDGNRIW GKDLKGIQLC HVTWSADSKI LLFGMANGEI
HIYDNQGNFI MKMKLNCLVN VTGAISIAGI HWYHGTEGYV EPDCPCLAIC FDNGRCQIMR
HENDQNPVLI DTGMYVVGIQ WNHIGSVLAV AGSQKVVTQD KDVNIVQFYT PFGEHLGTLK
VPGKQMCSLS WEGGGLKIAL AVDSFIYFAN IRPDYKWGYC SNTVVYAYTR PDRPEYCVVF
WDTKNNEKYV KYVKSLISIT TCGDFCILAT KADENHPQFV LVLCNSIGTP LDPKYIDLVP
LFVAMTKTHV IAASKEALYT WQYRVAKKLT ALEINQITRS RKEGRERIYH VDDVPSGSVD
GVFDYSKAIQ GTRDPICAIT ASDKTLIVGR ESGVIQRYSF PNVALIQKYS LDCRACQLSL
NCNSSRLAII DIAGVLTFFD LDTRVTDSTG QQVVGELLKL ERKDVWDMKW AKDNPDLFAM
MEKTRMYVFR NLDPEEPIQT SGYICNFEDL EIKSVLLDEI LKNPEHPSKD YIMNFEIRSL
RDSRALIEKV GIEDASQFIE DNPHPRLWRL LAEAALQKLD LYTAQQAFVR CKDYQGIKFV
KRLGNLQSES MKQAEVIAYF GRFEEAERMY LDMDRRDLAI GLRLKLGDWF RVLQLLKTGS
GDADDSLLEQ AHNAIGDYFA DRQKWMNAVQ YYVKGRNQER LAECYYMLED YEGLENLANS
LPENHKLLPE IAQMFVRVGM CEQAVSAFLK CNQPKAAVDT CVHLNQWNKA VELAKSHSMK
EIGSLLARYA SHLLEKNKTL DAIELYRKAS YFFDAAKLMY KIADEEAKKR TKPLRVKKLY
VLSALLIEQY HEQMKNAQRG KVKGKNSEAT SALAGLLEEE VLSTTSRFTD NAWRGAEAYH
FFILAQRQLY EGYVDTALKT ALHLRDYEDI IPSVEIYSLL ALCACASRAF GTCSKAFIKL
ESLETLSAEQ KQQYEDLALE IFTKHTPKDN RKSELNSLLE GGEGKLPTCI ATGSPIIEYQ
FWVCKVCKHY VLAQEISNYN FCPLCHSSVE
//
