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Database: UniProt
Entry: A6TI05_KLEP7
LinkDB: A6TI05_KLEP7
Original site: A6TI05_KLEP7 
ID   A6TI05_KLEP7            Unreviewed;       548 AA.
AC   A6TI05;
DT   21-AUG-2007, integrated into UniProtKB/TrEMBL.
DT   21-AUG-2007, sequence version 1.
DT   27-MAR-2024, entry version 89.
DE   RecName: Full=Energy-dependent translational throttle protein EttA {ECO:0000256|HAMAP-Rule:MF_00847};
DE            EC=3.6.1.- {ECO:0000256|HAMAP-Rule:MF_00847};
DE   AltName: Full=Translational regulatory factor EttA {ECO:0000256|HAMAP-Rule:MF_00847};
GN   Name=yjjK {ECO:0000313|EMBL:ABR80189.1};
GN   Synonyms=ettA {ECO:0000256|HAMAP-Rule:MF_00847};
GN   ORFNames=KPN_04846 {ECO:0000313|EMBL:ABR80189.1};
OS   Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=272620 {ECO:0000313|EMBL:ABR80189.1, ECO:0000313|Proteomes:UP000000265};
RN   [1] {ECO:0000313|EMBL:ABR80189.1, ECO:0000313|Proteomes:UP000000265}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578 {ECO:0000313|Proteomes:UP000000265};
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2] {ECO:0000313|EMBL:ABR80189.1, ECO:0000313|Proteomes:UP000000265}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700721 / MGH 78578 {ECO:0000313|Proteomes:UP000000265};
RG   The Klebsiella pneumonia Genome Sequencing Project;
RA   McClelland M., Sanderson E.K., Spieth J., Clifton W.S., Latreille P.,
RA   Sabo A., Pepin K., Bhonagiri V., Porwollik S., Ali J., Wilson R.K.;
RL   Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: A translation factor that gates the progression of the 70S
CC       ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site)
CC       into the translation elongation cycle by using a mechanism sensitive to
CC       the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates
CC       the state of the translating ribosome during subunit translocation. ATP
CC       hydrolysis probably frees it from the ribosome, which can enter the
CC       elongation phase. {ECO:0000256|HAMAP-Rule:MF_00847}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; Evidence={ECO:0000256|HAMAP-
CC         Rule:MF_00847};
CC   -!- SUBUNIT: Monomer. Probably contacts ribosomal proteins L1, L5, L33 and
CC       S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet).
CC       {ECO:0000256|HAMAP-Rule:MF_00847}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00847}.
CC       Note=Associates with ribosomes and polysomes. {ECO:0000256|HAMAP-
CC       Rule:MF_00847}.
CC   -!- DOMAIN: The P-site tRNA interaction motif (PtIM domain) probably
CC       interacts with the P-site tRNA(fMet) as well as the 23S rRNA.
CC       {ECO:0000256|HAMAP-Rule:MF_00847}.
CC   -!- DOMAIN: The arm domain is inserted in the first ABC transporter domain.
CC       Probably contacts ribosomal protein L1. {ECO:0000256|HAMAP-
CC       Rule:MF_00847}.
CC   -!- SIMILARITY: Belongs to the ABC transporter superfamily. ABCF family.
CC       Translational throttle EttA subfamily. {ECO:0000256|ARBA:ARBA00005868,
CC       ECO:0000256|HAMAP-Rule:MF_00847}.
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DR   EMBL; CP000647; ABR80189.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6TI05; -.
DR   STRING; 272620.KPN_04846; -.
DR   jPOST; A6TI05; -.
DR   PaxDb; 272620-KPN_04846; -.
DR   EnsemblBacteria; ABR80189; ABR80189; KPN_04846.
DR   KEGG; kpn:KPN_04846; -.
DR   HOGENOM; CLU_000604_36_0_6; -.
DR   Proteomes; UP000000265; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016887; F:ATP hydrolysis activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043022; F:ribosome binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0019843; F:rRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0000049; F:tRNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0045900; P:negative regulation of translational elongation; IEA:UniProtKB-UniRule.
DR   GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR   CDD; cd03221; ABCF_EF-3; 2.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR   HAMAP; MF_00847; EttA; 1.
DR   InterPro; IPR003593; AAA+_ATPase.
DR   InterPro; IPR032781; ABC_tran_Xtn.
DR   InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR   InterPro; IPR017871; ABC_transporter-like_CS.
DR   InterPro; IPR022374; EttA.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   NCBIfam; TIGR03719; ABC_ABC_ChvD; 1.
DR   PANTHER; PTHR43858; ENERGY-DEPENDENT TRANSLATIONAL THROTTLE PROTEIN ETTA; 1.
DR   PANTHER; PTHR43858:SF1; NON-TRANSPORTER ABC PROTEIN; 1.
DR   Pfam; PF00005; ABC_tran; 2.
DR   Pfam; PF12848; ABC_tran_Xtn; 1.
DR   SMART; SM00382; AAA; 2.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR   PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR   PROSITE; PS50893; ABC_TRANSPORTER_2; 2.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW   Rule:MF_00847}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00847};
KW   Hydrolase {ECO:0000256|HAMAP-Rule:MF_00847};
KW   Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW   Rule:MF_00847}; Protein biosynthesis {ECO:0000256|HAMAP-Rule:MF_00847};
KW   Repeat {ECO:0000256|HAMAP-Rule:MF_00847};
KW   RNA-binding {ECO:0000256|HAMAP-Rule:MF_00847};
KW   rRNA-binding {ECO:0000256|ARBA:ARBA00022730, ECO:0000256|HAMAP-
KW   Rule:MF_00847};
KW   Translation regulation {ECO:0000256|ARBA:ARBA00022845, ECO:0000256|HAMAP-
KW   Rule:MF_00847};
KW   tRNA-binding {ECO:0000256|ARBA:ARBA00022555, ECO:0000256|HAMAP-
KW   Rule:MF_00847}.
FT   DOMAIN          2..252
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000259|PROSITE:PS50893"
FT   DOMAIN          317..543
FT                   /note="ABC transporter"
FT                   /evidence="ECO:0000259|PROSITE:PS50893"
FT   REGION          88..132
FT                   /note="Arm"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00847"
FT   REGION          235..315
FT                   /note="PtIM"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00847"
FT   BINDING         32..39
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_label="1"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00847"
FT   BINDING         349..356
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /ligand_label="2"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_00847"
SQ   SEQUENCE   548 AA;  61396 MW;  E90BBFF41782327A CRC64;
     MHRVGKVVPP KRHILKNISL SFFPGAKIGV LGLNGAGKST LLRIMAGIDT EIEGEARPQP
     GIKIGYLPQE PQLNPEHTVR ESVEEAVAEV VNALKGLDEV YAKYAEPDAD FDKLAAQQGK
     FEEIIQAHDG HNLNVQLERA ADALRLPDWD AKIANLSGGE RRRVALCRLL LEKPDMLLLD
     EPTNHLDAES VAWLERFLHD FEGTVVAITH DRYFLDNVAG WILELDRGEG IPWEGNYSSW
     LEQKDQRLAQ EASQEAARRK SIEKELEWVR QGAKGRQSKG KARLARFEEL NNVEYQKRNE
     TNELFIPPGP RLGDKVLEVS HLRKSYGDRV LIDDLSFSVP KGAIVGIIGP NGAGKSTLFR
     MMSGQEQPDS GTITLGETVK LASVDQFRDA MDNSKTVWEE VSGGLDIMKI GNTEMPSRAY
     VGRFNFKGVD QGKRVGELSG GERGRLHLAK LLQVGGNVLL LDEPTNDLDI ETLRALENAL
     LEFPGCAMVI SHDRWFLDRI ATHILDYQDE GKVEFFEGNF TEYEEYKKRT LGADALEPKR
     IKYKRIAK
//
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