ID   A7J3S5_LEUCI            Unreviewed;       225 AA.
AC   A7J3S5;
DT   11-SEP-2007, integrated into UniProtKB/TrEMBL.
DT   11-SEP-2007, sequence version 1.
DT   24-JAN-2024, entry version 41.
DE   RecName: Full=dextransucrase {ECO:0000256|ARBA:ARBA00012592};
DE            EC=2.4.1.5 {ECO:0000256|ARBA:ARBA00012592};
DE   Flags: Fragment;
OS   Leuconostoc citreum.
OC   Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Leuconostoc.
OX   NCBI_TaxID=33964 {ECO:0000313|EMBL:ABK41040.1};
RN   [1] {ECO:0000313|EMBL:ABK41040.1}
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=1.12 {ECO:0000313|EMBL:ABK41040.1};
RX   PubMed=17716765; DOI=10.1016/j.ijfoodmicro.2007.07.014;
RA   Van der Meulen R., Grosu-Tudor S., Mozzi F., Vaningelgem F., Zamfir M.,
RA   Font de Valdez G., De Vuyst L.;
RT   "Screening of lactic acid bacteria isolates from dairy and cereal products
RT   for exopolysaccharide production and genes involved.";
RL   Int. J. Food Microbiol. 118:250-258(2007).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->6)-alpha-D-glucosyl](n) + sucrose = [(1->6)-alpha-D-
CC         glucosyl](n+1) + D-fructose; Xref=Rhea:RHEA:18825, Rhea:RHEA-
CC         COMP:11144, Rhea:RHEA-COMP:11145, ChEBI:CHEBI:17992,
CC         ChEBI:CHEBI:18269, ChEBI:CHEBI:37721; EC=2.4.1.5;
CC         Evidence={ECO:0000256|ARBA:ARBA00001152};
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 70 family.
CC       {ECO:0000256|ARBA:ARBA00009247}.
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DR   EMBL; DQ873510; ABK41040.1; -; Genomic_DNA.
DR   AlphaFoldDB; A7J3S5; -.
DR   CAZy; GH70; Glycoside Hydrolase Family 70.
DR   GO; GO:0047849; F:dextransucrase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046527; F:glucosyltransferase activity; IEA:InterPro.
DR   GO; GO:0009250; P:glucan biosynthetic process; IEA:InterPro.
DR   Gene3D; 3.20.20.470; Glucansucrase; 1.
DR   InterPro; IPR003318; Glyco_hydro70cat.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   Pfam; PF02324; Glyco_hydro_70; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
PE   3: Inferred from homology;
FT   DOMAIN          4..225
FT                   /note="Glycoside hydrolase family 70 catalytic"
FT                   /evidence="ECO:0000259|Pfam:PF02324"
FT   NON_TER         1
FT                   /evidence="ECO:0000313|EMBL:ABK41040.1"
FT   NON_TER         225
FT                   /evidence="ECO:0000313|EMBL:ABK41040.1"
SQ   SEQUENCE   225 AA;  25864 MW;  229A194DCC39AFEE CRC64;
     EFALDNRNPT VQAEQLNWLH YLMNFGSITA NDSAANFDGI RVDAVDNVDA DLLQIAADYF
     KAAYGVDKND ATANQHLSIL EDWSHNDPEY VKDFGSNQLT MDDYMHTQLI WSLTKDMRMR
     GTMQRFMDYY LVNRNHDSTE NTAIPNYSFV RARDSEVQTV IAQIISELHP DVKNSLAPTA
     DQLAEAFKVY NNDEKQADKK YTQYNMPSAY AMLLTNKDTV PRVYY
//