ID A8BZZ3_9INFA Unreviewed; 483 AA.
AC A8BZZ3;
DT 13-NOV-2007, integrated into UniProtKB/TrEMBL.
DT 13-NOV-2007, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=RNA-directed RNA polymerase catalytic subunit {ECO:0000256|ARBA:ARBA00020035};
DE EC=2.7.7.48 {ECO:0000256|ARBA:ARBA00012494};
DE Flags: Fragment;
GN Name=PB1 {ECO:0000313|EMBL:ABV48309.1};
OS Influenza A virus (A/pheasant/Shantou/7315/2004(H9N2)).
OC Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina;
OC Insthoviricetes; Articulavirales; Orthomyxoviridae; Alphainfluenzavirus;
OC Alphainfluenzavirus influenzae; Influenza A virus.
OX NCBI_TaxID=456677 {ECO:0000313|EMBL:ABV48309.1};
RN [1] {ECO:0000313|EMBL:ABV48309.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/pheasant/Shantou/7315/2004 {ECO:0000313|EMBL:ABV48309.1};
RX PubMed=17652402; DOI=10.1128/JVI.00979-07;
RA Xu K.M., Smith G.J., Bahl J., Duan L., Tai H., Vijaykrishna D., Wang J.,
RA Zhang J.X., Li K.S., Fan X.H., Webster R.G., Chen H., Peiris J.S., Guan Y.;
RT "The genesis and evolution of H9N2 influenza viruses in poultry from
RT southern China, 2000 to 2005.";
RL J. Virol. 81:10389-10401(2007).
RN [2] {ECO:0000313|EMBL:ABV48309.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=A/pheasant/Shantou/7315/2004 {ECO:0000313|EMBL:ABV48309.1};
RA Xu K., Smith G., Bahl J., Duan L., Tai H., Vijaykrishna D., Wang J.,
RA Zhang J., Li K., Fan X., Webster R., Chen H., Peiris J., Guan Y.;
RL Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: RNA-dependent RNA polymerase which is responsible for
CC replication and transcription of virus RNA segments. The transcription
CC of viral mRNAs occurs by a unique mechanism called cap-snatching. 5'
CC methylated caps of cellular mRNAs are cleaved after 10-13 nucleotides
CC by PA. In turn, these short capped RNAs are used as primers by PB1 for
CC transcription of viral mRNAs. During virus replication, PB1 initiates
CC RNA synthesis and copy vRNA into complementary RNA (cRNA) which in turn
CC serves as a template for the production of more vRNAs.
CC {ECO:0000256|ARBA:ARBA00002148}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}. Host
CC cytoplasm {ECO:0000256|ARBA:ARBA00004192}. Host nucleus
CC {ECO:0000256|ARBA:ARBA00004147}. Nucleus
CC {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; CY024438; ABV48309.1; -; Viral_cRNA.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0039523; P:suppression by virus of host mRNA transcription via inhibition of RNA polymerase II activity; IEA:UniProtKB-KW.
DR GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR GO; GO:0019083; P:viral transcription; IEA:UniProtKB-KW.
DR InterPro; IPR007099; RNA-dir_pol_NSvirus.
DR InterPro; IPR001407; RNA_pol_PB1_influenza.
DR Pfam; PF00602; Flu_PB1; 1.
DR PROSITE; PS50525; RDRP_SSRNA_NEG_SEG; 1.
PE 4: Predicted;
KW Eukaryotic host gene expression shutoff by virus
KW {ECO:0000256|ARBA:ARBA00023247};
KW Eukaryotic host transcription shutoff by virus
KW {ECO:0000256|ARBA:ARBA00022731};
KW Host cytoplasm {ECO:0000256|ARBA:ARBA00023200};
KW Host gene expression shutoff by virus {ECO:0000256|ARBA:ARBA00022995};
KW Host nucleus {ECO:0000256|ARBA:ARBA00022562};
KW Host-virus interaction {ECO:0000256|ARBA:ARBA00022581};
KW Inhibition of host RNA polymerase II by virus
KW {ECO:0000256|ARBA:ARBA00023103}; Membrane {ECO:0000256|SAM:Phobius};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW RNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022484};
KW Transferase {ECO:0000256|ARBA:ARBA00022484};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Viral RNA replication {ECO:0000256|ARBA:ARBA00022953};
KW Viral transcription {ECO:0000256|ARBA:ARBA00023314}.
FT TRANSMEM 406..426
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 286..483
FT /note="RdRp catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50525"
FT NON_TER 483
FT /evidence="ECO:0000313|EMBL:ABV48309.1"
SQ SEQUENCE 483 AA; 54951 MW; 4BDAFF6F52C27B01 CRC64;
MDVNPTLLFL KVPVQNAIST TFPYTGDPPY SHGTGTGYTM DTVNRTHQYS EKGKWTTNTE
TKAPQLNPID GPLPDNNEPS GYAQTDCVLE AMASLEESHP GIFENSCLET IEVVQQTRVD
KLTQGRQTYD WTLNRNQPAA TALANTIEVF RSNGLTTNES GRLIDFLKDV MDSMNKEEME
ITTHFQRKRR VRDNMTKKMV TQRTIGKKKQ RLNRRSYLIR ALTLNTMTKD AERGKLKRRA
IATPGMQIRG FVFFVETLAR SICEKLEQSG LPVGGNEKKA KLANVVRKMM TNSQDTELSF
TITGDNTKWN ENQNPRVFLA MITYITRNQP EWFRNVLSIA PIMFSNKMAR LGKGYMFESK
IMKLRTQIPA EMLATIDLKY FNESTRQKIE KIRPLLIEGT ASLSPGMMMG MFNMLSTVLG
VSILNLGQKK YTKTTYWWDG LQSSDDFALI INAPNYEGIQ AGVDRFYRTC KLVGINMSKK
KSY
//
